{"id":588,"date":"2019-06-10T15:29:56","date_gmt":"2019-06-10T15:29:56","guid":{"rendered":"http:\/\/niksimoh.ir\/%d8%aa%da%a9%d9%86%db%8c%da%a9-touchdown-pcr\/"},"modified":"2020-04-07T11:56:35","modified_gmt":"2020-04-07T08:26:35","slug":"touchdown_pcr__technique","status":"publish","type":"post","link":"https:\/\/darwino.ir\/blogs\/touchdown_pcr__technique\/","title":{"rendered":"\u062a\u06a9\u0646\u06cc\u06a9 Touchdown PCR – \u0631\u0648\u0634 \u06a9\u0627\u0631\/\u067e\u0631\u0648\u062a\u06a9\u0644"},"content":{"rendered":"

\u270d <\/strong>\u06a9\u0627\u0631\u0628\u0631\u062f \u062a\u06a9\u0646\u06cc\u06a9 Touchdown PCR \u062f\u0631 \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc\u00a0<\/strong><\/h2>\n

\"\"<\/p>\n

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\u062f\u0631 \u0648\u0627\u0642\u0639 \u0627\u0633\u0627\u0633 <\/span>Touchdown PCR<\/span> \u062a\u063a\u06cc\u06cc\u0631 \u067e\u0627\u0631\u0627\u0645\u062a\u0631\u0647\u0627\u06cc \u0645\u0631\u0628\u0648\u0637 \u0628\u0647 \u0647\u0631 \u0633\u06cc\u06a9\u0644 \u0627\u0633\u062a \u0648 \u062a\u0641\u0627\u0648\u062a \u0622\u0646 \u0628\u0627 <\/span>PCR<\/span> \u0645\u0639\u0645\u0648\u0644\u06cc \u062f\u0648 \u0641\u0627\u0632 \u062c\u062f\u0627\u06af\u0627\u0646\u0647 \u0686\u0631\u062e\u0647 \u062f\u0645\u0627\u06cc\u06cc \u0627\u0633\u062a\u060c \u0641\u0627\u0632 \u0627\u0648\u0644 \u0641\u0627\u0632 <\/span>Touchdown<\/span> \u0646\u0627\u0645\u06cc\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f \u06a9\u0647 \u0628\u0627 \u062f\u0645\u0627\u06cc \u0627\u062a\u0635\u0627\u0644 \u0628\u0627\u0644\u0627\u062a\u0631 \u0627\u0632 <\/span>Tm<\/span> \u00a0<\/span><\/span>Tm+10\u00b0C)<\/span>) \u0634\u0631\u0648\u0639 \u00a0<\/span>\u0648 \u062f\u0631 \u062d\u06cc\u0646 \u0633\u06cc\u06a9\u0644 \u0647\u0627\u06cc \u0645\u062a\u0648\u0627\u0644\u06cc \u06a9\u0627\u0647\u0634 \u0645\u06cc \u06cc\u0627\u0628\u062f \u062a\u0627 \u0628\u0647 \u062f\u0645\u0627\u06cc \u0627\u062a\u0635\u0627\u0644 \u0645\u0648\u0631\u062f \u0646\u0638\u0631 \u0628\u0631\u0633\u06cc\u0645 \u0633\u067e\u0633 \u0641\u0627\u0632 \u062f\u0648\u0645 \u0647\u0645\u0627\u0646\u00a0 \u062a\u06a9\u0646\u06cc\u06a9 <\/span>PCR<\/span> \u0645\u0639\u0645\u0648\u0644\u06cc \u062f\u0631\u0627\u06cc\u0646 \u062f\u0645\u0627\u0633\u062a \u067e\u0633 \u0645\u06cc \u062a\u0648\u0627\u0646 \u06af\u0641\u062a \u062f\u0631\u0637\u06cc \u06f1\u06f0 \u062a\u0627 \u06f1\u06f5 \u0633\u06cc\u06a9\u0644 \u0627\u0648\u0644\u06cc\u0647 \u00a0<\/span>\u0648\u0627\u06a9\u0646\u0634 \u062f\u0645\u0627\u0647\u0627\u06cc \u0628\u0627\u0644\u0627\u062a\u0631 \u0627\u0632 \u062f\u0645\u0627\u06cc \u0627\u062a\u0635\u0627\u0644 <\/span>annealing<\/span> \u067e\u06cc\u0634 \u0628\u06cc\u0646\u06cc \u0634\u062f\u0647 \u0628\u0647 \u06a9\u0627\u0631 \u0645\u06cc \u0631\u0648\u062f \u0648 \u0628\u0647 \u0645\u0631\u0648\u0631 \u06a9\u0627\u0647\u0634 \u0645\u06cc \u06cc\u0627\u0628\u062f \u062a\u0627 \u0628\u0647 \u062f\u0645\u0627\u06cc <\/span>\u00a0<\/span><\/span>Tm<\/span>\u0645\u0648\u0631\u062f \u0646\u0638\u0631 \u06a9\u0647 \u0647\u0645\u0627\u0646 \u062f\u0645\u0627\u06cc <\/span>Touchdown <\/span>\u00a0<\/span>\u0628\u0647 \u0622\u0646 \u06af\u0641\u062a\u0647 \u0645\u06cc \u0634\u0648\u062f \u0628\u0631\u0633\u06cc\u0645 \u0648 \u0633\u067e\u0633 \u06f2\u06f5-\u06f2\u06f0 \u0633\u06cc\u06a9\u0644 \u0628\u0627 \u062f\u0645\u0627\u06cc \u0627\u062a\u0635\u0627\u0644 \u0645\u0648\u0631\u062f \u0646\u0638\u0631 \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u06af\u06cc\u0631\u062f \u0628\u0627\u06cc\u062f \u062f\u0642\u062a \u06a9\u0631\u062f \u06a9\u0647 \u062a\u0639\u062f\u0627\u062f \u06a9\u0644\u06cc \u0633\u06cc\u06a9\u0644 \u0647\u0627 \u0628\u06cc\u0634\u062a\u0631 \u0627\u0632 \u06f3\u06f5 \u0633\u06cc\u06a9\u0644 \u0646\u0634\u0648\u062f \u0686\u0648\u0646 \u062e\u0648\u062f \u0627\u06cc\u0646 \u0645\u0648\u0636\u0648\u0639 \u0628\u0627\u0639\u062b \u0627\u0641\u0632\u0627\u06cc\u0634 \u062a\u06a9\u062b\u06cc\u0631 \u0628\u0627\u0646\u062f\u0647\u0627\u06cc \u063a\u06cc\u0631 \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u0648 \u062f\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc \u067e\u0631\u0627\u06cc\u0645\u0631 \u0645\u06cc \u0634\u0648\u062f . <\/span>\u062f\u0645\u0627\u0647\u0627\u06cc \u0628\u0627\u0644\u0627\u06cc\u06cc \u06a9\u0647 \u062f\u0631 \u0633\u06cc\u06a9\u0644 \u0647\u0627\u06cc \u0627\u0628\u062a\u062f\u0627\u06cc\u06cc \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc \u06a9\u0646\u06cc\u0645 \u0627\u062d\u062a\u0645\u0627\u0644 \u0627\u062a\u0635\u0627\u0644 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627 \u0628\u0647 \u0646\u0648\u0627\u062d\u06cc \u0627\u0632 \u062a\u0648\u0627\u0644\u06cc \u0627\u0644\u06af\u0648 \u06a9\u0647 \u0628\u06cc\u0634\u062a\u0631\u06cc\u0646 \u0645\u06cc\u0632\u0627\u0646 \u0645\u06a9\u0645\u0644 \u0628\u0648\u062f\u0646 \u0631\u0627 \u062f\u0627\u0631\u0646\u062f \u0627\u0641\u0632\u0627\u06cc\u0634 \u0645\u06cc \u062f\u0647\u062f \u0648 \u062f\u0631 \u0646\u062a\u06cc\u062c\u0647 \u062f\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc \u067e\u0631\u0627\u06cc\u0645\u0631 \u0648 \u0627\u062a\u0635\u0627\u0644\u0627\u062a \u063a\u06cc\u0631\u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u0628\u0647 \u062d\u062f\u0627\u0642\u0644 \u0645\u06cc \u0631\u0633\u062f \u0648\u0644\u06cc \u0645\u0634\u06a9\u0644 \u0627\u06cc\u0646 \u062f\u0645\u0627\u06cc \u0628\u0627\u0644\u0627 \u0627\u0641\u0632\u0627\u06cc\u0634 \u0627\u062d\u062a\u0645\u0627\u0644 \u062c\u062f\u0627 \u0634\u062f\u0646 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627 \u0627\u0632 \u062a\u0648\u0627\u0644\u06cc \u0645\u0648\u0631\u062f \u0646\u0638\u0631 \u0648 \u06a9\u0627\u0647\u0634 \u0645\u062d\u0635\u0648\u0644 \u0627\u0633\u062a \u0648\u0644\u06cc \u06a9\u0627\u0647\u0634 \u062f\u0645\u0627 \u0628\u0647 \u0635\u0648\u0631\u062a \u062a\u062f\u0631\u06cc\u062c\u06cc \u062f\u0631 \u0633\u06cc\u06a9\u0644 \u0647\u0627\u06cc \u0628\u0639\u062f\u06cc \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u062a\u0648\u0644\u06cc\u062f \u0634\u062f\u0647 \u062f\u0631 \u0627\u0628\u062a\u062f\u0627\u06cc \u0648\u0627\u06a9\u0646\u0634 \u0645\u0627\u0646\u0639 \u0627\u0632 \u06a9\u0627\u0647\u0634 \u0645\u062d\u0635\u0648\u0644 \u0645\u06cc \u0634\u0648\u062f \u0648 \u062f\u0631 \u0646\u0647\u0627\u06cc\u062a \u0628\u0627 \u067e\u06cc\u0634\u0631\u0641\u062a \u0648\u0627\u06a9\u0646\u0634 \u0627\u06cc\u0646 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u0628\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u063a\u06cc\u0631 \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u0648 \u062f\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc \u067e\u0631\u0627\u06cc\u0645\u0631 \u063a\u0644\u0628\u0647 \u0645\u06cc \u06a9\u0646\u0646\u062f.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u0627\u0633\u0627\u0633 Touchdown PCR \u062a\u063a\u06cc\u06cc\u0631 \u067e\u0627\u0631\u0627\u0645\u062a\u0631\u0647\u0627\u06cc \u0645\u0631\u0628\u0648\u0637 \u0628\u0647 \u0647\u0631 \u0633\u06cc\u06a9\u0644 \u0647\u0633\u062a \u0648 \u062a\u0641\u0627\u0648\u062a \u0622\u0646 \u0628\u0627 PCR \u0645\u0639\u0645\u0648\u0644\u06cc \u062f\u0648 \u0641\u0627\u0632 \u062c\u062f\u0627\u06af\u0627\u0646\u0647 \u0686\u0631\u062e\u0647 \u062f\u0645\u0627\u06cc\u06cc \u0627\u0633\u062a\u060c \u0641\u0627\u0632 \u0627\u0648\u0644 \u0641\u0627\u0632 Touchdown \u0646\u0627\u0645\u06cc\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f .<\/p>\n","protected":false},"author":6,"featured_media":1236,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[30],"tags":[881,962,963,564,779,1936,1938,1937,531,1935],"blog-category":[3070,3071],"darwinoplus-category":[],"class_list":{"0":"post-588","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","6":"hentry","7":"category-blog","8":"tag-_pcr","9":"tag-_touchdown_pcr","10":"tag-__annealing","11":"tag-___","13":"tag-___touchdown_pcr","15":"tag-_pdf__touchdown_pcr","16":"tag-__real_time_pcr","17":"tag-____touchdown_pcr","18":"blog-category-genetic","19":"blog-category-molecular"},"_links":{"self":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/588","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/comments?post=588"}],"version-history":[{"count":10,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/588\/revisions"}],"predecessor-version":[{"id":22010,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/588\/revisions\/22010"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media\/1236"}],"wp:attachment":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media?parent=588"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/categories?post=588"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/tags?post=588"},{"taxonomy":"blog-category","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/blog-category?post=588"},{"taxonomy":"darwinoplus-category","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/darwinoplus-category?post=588"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}