{"id":537,"date":"2019-06-10T15:27:27","date_gmt":"2019-06-10T15:27:27","guid":{"rendered":"http:\/\/niksimoh.ir\/%d8%aa%da%a9%d9%86%db%8c%da%a9-pcr\/"},"modified":"2021-07-12T12:56:45","modified_gmt":"2021-07-12T09:26:45","slug":"pcr-technique","status":"publish","type":"post","link":"https:\/\/darwino.ir\/blogs\/pcr-technique\/","title":{"rendered":"\u062a\u06a9\u0646\u06cc\u06a9 \u0648\u0627\u06a9\u0646\u0634 \u0632\u0646\u062c\u06cc\u0631\u0647 \u0627\u06cc \u067e\u0644\u06cc\u0645\u0631\u0627\u0632 (PCR)"},"content":{"rendered":"<h2><strong>\u270d<\/strong><strong>PCR <\/strong><strong>\u0686\u06cc\u0633\u062a \u0648 \u0686\u0647 \u06a9\u0627\u0631\u0628\u0631\u062f\u0647\u0627\u06cc\u06cc \u062f\u0627\u0631\u062f\u061f<\/strong><\/h2>\n<p><strong>PCR\u060c \u0631\u0648\u0634\u06cc \u063a\u06cc\u0631 \u0627\u0632 \u06a9\u0644\u0648\u0646 \u0633\u0627\u0632\u06cc \u0627\u0633\u062a \u06a9\u0647 \u0645\u0642\u0627\u062f\u06cc\u0631 \u0646\u0627\u0645\u062d\u062f\u0648\u062f\u06cc \u0627\u0632 \u062a\u0648\u0627\u0644\u06cc \u0645\u0648\u0631\u062f\u0646\u0638\u0631 \u0631\u0627 \u062a\u0648\u0644\u06cc\u062f \u0645\u06cc \u06a9\u0646\u062f<\/strong><\/p>\n<p>\u0627\u06cc\u0646 \u062a\u06a9\u0646\u06cc\u06a9 \u0628\u0631\u0627\u06cc \u0628\u0631\u0631\u0633\u06cc \u062d\u0636\u0648\u0631 \u06cc\u0627 \u0639\u062f\u0645 \u062d\u0636\u0648\u0631 \u06cc\u06a9 \u0698\u0646 \u062e\u0627\u0635 \u062f\u0631 \u0645\u062e\u0644\u0648\u0637\u06cc \u0627\u0632 DNA \u0647\u0627\u060c \u062a\u0634\u062e\u06cc\u0635 \u0647\u0627\u06cc \u0642\u0628\u0644 \u0627\u0632 \u062a\u0648\u0644\u062f (\u062a\u0639\u06cc\u06cc\u0646 \u062c\u0646\u0633\u06cc\u062a\u060c \u0628\u06cc\u0645\u0627\u0631\u06cc \u0647\u0627) \u0648&#8230; \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f. \u062a\u06a9\u0646\u06cc\u06a9 PCR \u0647\u0645\u0627\u0646 \u0647\u0645\u0627\u0646\u0646\u062f\u0633\u0627\u0632\u06cc \u062f\u0631 \u0634\u0631\u0627\u06cc\u0637 in vitro \u0627\u0633\u062a \u062f\u0631 \u0648\u0627\u0642\u0639 \u06cc\u0639\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0622\u0646\u0632\u06cc\u0645\u06cc \u06cc\u06a9 \u0642\u0637\u0639\u0647 \u0627\u0632 DNA \u06a9\u0647 \u0628\u06cc\u0646 \u062f\u0648 \u0627\u0644\u06cc\u06af\u0648\u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f (\u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627) \u0642\u0631\u0627\u0631 \u06af\u0631\u0641\u062a\u0647 \u0628\u0646\u0627\u0628\u0631\u0627\u06cc\u0646 \u0628\u0627\u06cc\u062f \u0628\u0627 \u0628\u0647 \u06a9\u0627\u0631\u06af\u06cc\u0631\u06cc \u0639\u0644\u0645 \u0628\u06cc\u0648\u0627\u0646\u0641\u0648\u0631\u0645\u0627\u062a\u06cc\u06a9 \u062a\u0648\u0627\u0644\u06cc \u0627\u0637\u0631\u0627\u0641 \u0642\u0637\u0639\u0647 \u0645\u0648\u0631\u062f \u0646\u0638\u0631\u0645\u0627\u0646 \u0631\u0627 \u067e\u06cc\u062f\u0627 \u06a9\u0646\u06cc\u0645 \u0648 \u0628\u0627 \u0637\u0631\u0627\u062d\u06cc \u067e\u0631\u0627\u06cc\u0645\u0631 \u0628\u0631\u0627\u06cc \u0622\u0646\u0647\u0627 \u0648 \u0627\u0639\u0645\u0627\u0644 \u0633\u06cc\u06a9\u0644 \u0647\u0627\u06cc \u0645\u06a9\u0631\u0631 \u062d\u0631\u0627\u0631\u062a\u06cc \u0642\u0637\u0639\u0647 \u0631\u0627 \u062a\u06a9\u062b\u06cc\u0631 \u06a9\u0646\u06cc\u0645.<\/p>\n<p>\u0628\u0639\u062f \u0627\u0632 \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA \u06a9\u0647 \u062f\u0631 \u0645\u0637\u0627\u0644\u0628 \u0642\u0628\u0644 \u06af\u0641\u062a\u0647 \u0634\u062f\u060c \u06cc\u06a9 \u0633\u0631\u06cc \u0645\u0648\u0627\u062f \u0648 \u0648\u0633\u0627\u06cc\u0644 \u0628\u0631\u0627\u06cc PCR \u0644\u0627\u0632\u0645 \u062f\u0627\u0631\u06cc\u0645 \u0645\u062b\u0644: \u0628\u0627\u0641\u0631\u060c \u0622\u0646\u0632\u06cc\u0645 \u060cMgCl2 \u060cdNTP\u060c \u067e\u0631\u0627\u06cc\u0645\u0631\u060c\u0622\u0646\u0632\u06cc\u0645 \u00a0DNA \u067e\u0644\u06cc\u0645\u0631\u0627\u0632 Taq \u06cc\u0627 Pfu\u00a0 \u0645\u0642\u0627\u0648\u0645 \u0628\u0647 \u062d\u0631\u0627\u0631\u062a \u0648 .. \u0648 DNA \u0627\u0644\u06af\u0648 \u06a9\u0647 \u0647\u0645\u0647 \u06cc \u0627\u06cc\u0646 \u0645\u0648\u0627\u062f \u0648 \u062f\u0631 \u062a\u06cc\u0648\u0628 \u0645\u062e\u0635\u0648\u0635 \u0631\u06cc\u062e\u062a\u0647 \u0648 \u062f\u0631 \u062f\u0633\u062a\u06af\u0627\u0647 \u062a\u0631\u06a9\u0648\u0633\u0627\u06cc\u06a9\u0644\u0631 \u06a9\u0647 \u0633\u06cc\u06a9\u0644 \u0647\u0627\u06cc \u0645\u06a9\u0631\u0631 \u062d\u0631\u0627\u0631\u062a\u06cc \u0627\u0639\u0645\u0627\u0644 \u0645\u06cc \u06a9\u0646\u062f \u0642\u0631\u0627\u0631 \u0645\u06cc \u062f\u0647\u06cc\u0645.<\/p>\n<h3><strong>\u0645\u0631\u0627\u062d\u0644 \u06cc\u06a9 \u0633\u06cc\u06a9\u0644 <\/strong><strong>PCR<\/strong><strong>:<\/strong><\/h3>\n<p>\u0628\u0647 \u0647\u0631 \u0633\u06cc\u06a9\u0644 \u0627\u0632 PCR \u06cc\u06a9 Stage \u06af\u0641\u062a\u0647 \u0645\u06cc \u0634\u0648\u062f \u06a9\u0647 \u0647\u0631 Stage \u0634\u0627\u0645\u0644 \u0686\u0646\u062f\u06cc\u0646 Step \u0645\u06cc \u0628\u0627\u0634\u062f.<\/p>\n<p><strong>\u0645\u0631\u062d\u0644\u0647\u00a0 \u0648\u0627\u0633\u0631\u0634\u062a (<\/strong><strong>Denaturation Step)<\/strong><strong>:<\/strong> \u0645\u0631\u062d\u0644\u0647 \u0628\u0627\u0632 \u0634\u062f\u0646 \u062f\u0648 \u0631\u0634\u062a\u0647 DNA \u060c \u06a9\u0647 \u062f\u0631 \u0627\u06cc\u0646 \u0645\u0631\u062d\u0644\u0647 \u062f\u0645\u0627\u06cc \u062f\u0633\u062a\u06af\u0627\u0647 \u0631\u0627 \u0645\u0639\u0645\u0648\u0644\u0627 \u062a\u0627 \u062f\u0645\u0627\u06cc \u06f9\u06f4 \u062a\u0627 \u06f9\u06f8 \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u06af\u0631\u0627\u062f \u0628\u0627\u0644\u0627 \u0628\u0631\u062f\u0647 \u06a9\u0647 \u0627\u06cc\u0646 \u062f\u0645\u0627 \u0628\u0627\u0639\u062b \u06af\u0633\u0633\u062a\u0646 \u067e\u06cc\u0648\u0646\u062f \u0647\u0627\u06cc \u0647\u06cc\u062f\u0631\u0648\u0698\u0646\u06cc \u062f\u0648 \u0631\u0634\u062a\u0647 DNA\u00a0 \u0648 \u0646\u0647\u0627\u06cc\u062a\u0627 \u062f\u0646\u0627\u062a\u0648\u0631\u0647 \u0634\u062f\u0646 \u0645\u0648\u0644\u06a9\u0648\u0644 \u0645\u06cc \u0634\u0648\u062f. \u062d\u0631\u0627\u0631\u062a \u062f\u0631 \u0627\u06cc\u0646 \u0645\u0631\u062d\u0644\u0647 \u0628\u0647 \u0645\u06cc\u0632\u0627\u0646 \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f\u0647\u0627 \u062f\u0631 DNA \u0628\u0633\u062a\u06af\u06cc \u062f\u0627\u0631\u062f.<\/p>\n<p><strong>\u0645\u0631\u062d\u0644\u0647 \u0627\u062a\u0635\u0627\u0644 <\/strong><strong>(Annealing Step)<\/strong><strong>:<\/strong> \u062f\u0631 \u0627\u06cc\u0646 \u0645\u0631\u062d\u0644\u0647 \u062d\u0631\u0627\u0631\u062a \u062a\u0627 \u062f\u0645\u0627\u06cc \u06f5\u06f5-\u06f6\u06f5 \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u06af\u0631\u0627\u062f \u067e\u0627\u06cc\u06cc\u0646 \u0645\u06cc \u0622\u06cc\u062f \u060c \u062f\u0631 \u0627\u06cc\u0646 \u0645\u0631\u062d\u0644\u0647 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc \u0645\u0648\u062c\u0648\u062f \u062f\u0631 \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634 \u062f\u0631 \u0645\u062d\u0644 \u0647\u0627\u06cc \u0648\u06cc\u0698\u0647 \u0627\u06cc \u0627\u0632 \u0645\u0648\u0644\u06a9\u0648\u0644 DNA \u0627\u062a\u0635\u0627\u0644 \u067e\u06cc\u062f\u0627 \u0645\u06cc \u06a9\u0646\u0646\u062f. \u0627\u0644\u0628\u062a\u0647 \u062f\u0631 \u0627\u06cc\u0646 \u062f\u0645\u0627 \u0627\u0645\u06a9\u0627\u0646 \u062f\u0648 \u0631\u0634\u062a\u0647 \u0627\u06cc \u0634\u062f\u0646 \u0645\u062c\u062f\u062f DNA \u0648\u062c\u0648\u062f \u062f\u0627\u0631\u062f \u0648\u0644\u06cc \u0639\u0645\u062f\u062a\u0627 \u0627\u06cc\u0646 \u0627\u062a\u0641\u0627\u0642 \u0631\u062e \u0646\u0645\u06cc \u062f\u0647\u062f.<\/p>\n<p><strong>\u0645\u0631\u062d\u0644\u0647 \u067e\u0644\u064a\u0645\u0631\u064a\u0632\u0627\u0633\u064a\u0648\u0646 \u06cc\u0627 \u0637\u0648\u06cc\u0644 \u0634\u062f\u0646 (<\/strong><strong>Extension\/Elongation Step<\/strong><strong>): <\/strong>\u062f\u0631 \u0645\u0631\u062d\u0644\u0647 \u0633\u0648\u0645 \u062f\u0648\u0628\u0627\u0631\u0647 \u062f\u0645\u0627\u06cc \u062f\u0633\u062a\u06af\u0627\u0647 \u062a\u0627 \u06f7\u06f2 \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u06af\u0631\u0627\u062f \u0627\u0641\u0632\u0627\u06cc\u0634 \u067e\u06cc\u062f\u0627 \u0645\u06cc \u06a9\u0646\u062f \u06a9\u0647 \u0627\u06cc\u0646 \u062f\u0645\u0627 \u0645\u0646\u0627\u0633\u0628 \u062a\u0631\u06cc\u0646 \u062d\u0631\u0627\u0631\u062a \u0628\u0631\u0627\u06cc \u0639\u0645\u0644\u06a9\u0631\u062f \u0635\u062d\u06cc\u062d \u0622\u0646\u0632\u06cc\u0645 DNA \u067e\u0644\u06cc\u0645\u0631\u0627\u0632 Taq \u0645\u0648\u062c\u0648\u062f \u062f\u0631 \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634 \u0627\u0633\u062a. \u062f\u0631 \u0627\u06cc\u0646\u062c\u0627 \u0648\u0638\u06cc\u0641\u0647 \u0622\u0646\u0632\u06cc\u0645 \u067e\u0644\u06cc\u0645\u0631\u0627\u0632 \u0627\u06cc\u0646 \u0627\u0633\u062a \u06a9\u0647 \u0628\u0647 \u0647\u0631 \u067e\u0631\u0627\u06cc\u0645\u0631 \u0628\u0686\u0633\u0628\u062f \u0648 \u0627\u0632 DNA \u0627\u0644\u06af\u0648 \u0631\u0634\u062a\u0647 \u062c\u062f\u06cc\u062f\u06cc \u0628\u0633\u0627\u0632\u062f.<\/p>\n<p>\u0645\u0631\u0627\u062d\u0644 \u0628\u0639\u062f \u062f\u0631 \u0648\u0627\u06a9\u0646\u0634 \u0632\u0646\u062c\u06cc\u0631\u0647 \u0627\u06cc \u067e\u0644\u06cc\u0645\u0631\u0627\u0632 \u062f\u0631 \u0648\u0627\u0642\u0639 \u062a\u06a9\u0631\u0627\u0631 \u062a\u0645\u0627\u0645 \u0627\u06cc\u0646 \u0645\u0631\u0627\u062d\u0644 \u0627\u0632 \u0627\u0628\u062a\u062f\u0627 \u062a\u0627 \u0627\u0646\u062a\u0647\u0627 \u0627\u0633\u062a \u0628\u0647 \u0637\u0648\u0631\u06cc \u06a9\u0647 \u0628\u0627 \u062a\u06a9\u0631\u0627\u0631 \u0628\u0647 \u0645\u06cc\u0632\u0627\u0646 \u06f2\u06f5 \u062a\u0627 \u06f4\u06f0 \u0686\u0631\u062e\u0647\u060c \u0645\u06cc\u0632\u0627\u0646 DNA \u06a9\u067e\u06cc \u0634\u062f\u0647 \u0628\u0647 \u0628\u06cc\u0634 \u0627\u0632 \u06f1\u06f3\u06f0 \u0645\u06cc\u0644\u06cc\u0648\u0646 \u0645\u0648\u0644\u06a9\u0648\u0644 DNA \u062f\u0648 \u0631\u0634\u062a\u0647 \u0627\u06cc\u06cc \u0645\u06cc \u0631\u0633\u062f.<\/p>\n<p><strong>\u0645\u0631\u062d\u0644\u0647 \u0637\u0648\u06cc\u0644 \u0634\u062f\u0646 \u0646\u0647\u0627\u06cc\u06cc (<\/strong><strong>Final Elongation<\/strong><strong>):<\/strong> \u0627\u06cc\u0646 \u0645\u0631\u062d\u0644\u0647\u060c \u067e\u0633 \u0627\u0632 \u0622\u062e\u0631\u06cc\u0646 \u0633\u06cc\u06a9\u0644 \u00a0PCR \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f7\u06f2 \u062f\u0631\u062c\u0647 \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u0634\u0648\u062f\u060c \u062a\u0627 \u0627\u0637\u0645\u06cc\u0646\u0627\u0646 \u062d\u0627\u0635\u0644 \u0634\u0648\u062f \u06a9\u0647 \u0647\u0645\u0647 \u062a\u06a9 \u0631\u0634\u062a\u0647 \u0647\u0627\u06cc DNA \u0647\u0645\u0627\u0646\u0646\u062f \u0633\u0627\u0632\u06cc \u0634\u062f\u0647 \u0627\u0646\u062f.<\/p>\n<p><strong>\u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u0646\u0647\u0627\u06cc\u06cc <\/strong><strong>(Final hold)<\/strong><strong>:<\/strong> \u062f\u0631 \u0627\u06cc\u0646 \u0645\u0631\u062d\u0644\u0647\u060c \u0645\u062d\u0644\u0648\u0644 \u0646\u0647\u0627\u06cc\u06cc \u0628\u0647 \u0645\u062f\u062a \u06a9\u0648\u062a\u0627\u0647\u06cc \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f4 \u0627\u0644\u06cc \u06f1\u06f5 \u062f\u0631\u062c\u0647 \u0642\u0627\u0628\u0644 \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u0627\u0633\u062a.<\/p>\n<p><strong>\u062f\u0631 \u0646\u0647\u0627\u06cc\u062a \u0645\u06cc \u062a\u0648\u0627\u0646\u06cc\u0645 \u0635\u062d\u062a \u0627\u0646\u062c\u0627\u0645 PCR \u0631\u0627 \u0627\u0632 \u0637\u0631\u06cc\u0642 \u0627\u0644\u06a9\u062a\u0631\u0648\u0641\u0648\u0631\u0632 \u062f\u0631 \u0698\u0644 \u0622\u06af\u0627\u0631\u0632 \u06cc\u0627 \u067e\u0644\u06cc \u0627\u06a9\u0631\u06cc\u0644 \u0627\u0645\u06cc\u062f \u0628\u0631\u0631\u0633\u06cc \u06a9\u0646\u06cc\u0645.<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u0627\u06cc\u0646 \u062a\u06a9\u0646\u06cc\u06a9 \u0628\u0631\u0627\u06cc \u0628\u0631\u0631\u0633\u06cc \u062d\u0636\u0648\u0631 \u06cc\u0627 \u0639\u062f\u0645 \u062d\u0636\u0648\u0631 \u06cc\u06a9 \u0698\u0646 \u062e\u0627\u0635 \u062f\u0631 \u0645\u062e\u0644\u0648\u0637\u06cc \u0627\u0632 DNA \u0647\u0627 \u0648 \u062a\u0634\u062e\u06cc\u0635 \u0647\u0627\u06cc \u067e\u06cc\u0634 \u0627\u0632 \u062a\u0648\u0644\u062f \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc\u0634\u0647<\/p>\n","protected":false},"author":1,"featured_media":1055,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[30],"tags":[1777,881,1266,1772,564,882,1774,1776,1775,943,1268,1267,1527,1773],"blog-category":[],"darwinoplus-category":[],"class_list":{"0":"post-537","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","6":"hentry","7":"category-blog","8":"tag-_dna__taq","9":"tag-_pcr","10":"tag-____","11":"tag-_____pcr","12":"tag-___","14":"tag-______","15":"tag-__pcr","17":"tag-_","19":"tag-_in_vitro","20":"tag-_______"},"_links":{"self":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/537","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/comments?post=537"}],"version-history":[{"count":25,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/537\/revisions"}],"predecessor-version":[{"id":27453,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/537\/revisions\/27453"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media\/1055"}],"wp:attachment":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media?parent=537"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/categories?post=537"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/tags?post=537"},{"taxonomy":"blog-category","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/blog-category?post=537"},{"taxonomy":"darwinoplus-category","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/darwinoplus-category?post=537"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}