{"id":2928,"date":"2019-06-19T19:33:55","date_gmt":"2019-06-19T19:33:55","guid":{"rendered":"http:\/\/darwino.ir\/blogs\/?p=2928"},"modified":"2021-02-01T12:16:38","modified_gmt":"2021-02-01T08:46:38","slug":"rt-pcr-technique-for-synthesizng-dna","status":"publish","type":"post","link":"https:\/\/darwino.ir\/blogs\/rt-pcr-technique-for-synthesizng-dna\/","title":{"rendered":"\u062a\u06a9\u0646\u06cc\u06a9 RT_PCR \u062c\u0647\u062a \u0633\u0646\u062a\u0632 cDNA"},"content":{"rendered":"<h2><strong>\u270d <\/strong><strong>\u00a0<\/strong><strong>RT-PCR \u0686\u0647 \u062a\u06a9\u0646\u06cc\u06a9\u06cc \u0627\u0633\u062a \u0648 \u062f\u0631 \u0686\u0647 \u0645\u0648\u0627\u0631\u062f\u06cc \u06a9\u0627\u0631\u0628\u0631\u062f \u062f\u0627\u0631\u062f\u061f<\/strong><\/h2>\n<h3><strong>\u0627\u06cc\u0646 \u062a\u06a9\u0646\u06cc\u06a9\u00a0 \u0646\u0645\u0648\u0646\u0647 \u06cc \u0627\u0644\u06af\u0648\u06cc \u0634\u0631\u0648\u0639 \u0648\u0627\u06a9\u0646\u0634 \u062a\u06a9\u062b\u06cc\u0631 \u0627\u0633\u06cc\u062f\u0647\u0627\u06cc \u0646\u0648\u06a9\u0644\u0626\u06cc\u06a9 \u0628\u0647 \u062c\u0627\u06cc<\/strong><strong> DNA <\/strong><strong>\u0645\u0648\u0644\u06a9\u0648\u0644<\/strong><strong> RNA \u00a0<\/strong><strong>\u0645\u06cc \u0628\u0627\u0634\u062f \u06a9\u0647 \u0628\u0647 \u0622\u0646<\/strong><strong> RT-PCR (Reverse transcription PCR) <\/strong><strong>\u06af\u0641\u062a\u0647 \u0645\u06cc \u0634\u0648\u062f<\/strong><strong>.<\/strong><\/h3>\n<p>\u062f\u0631 \u0628\u0633\u06cc\u0627\u0631\u06cc \u0645\u0648\u0627\u0642\u0639 \u06a9\u0647 \u0645\u0645\u06a9\u0646 \u0627\u0633\u062a \u0646\u0645\u0648\u0646\u0647 \u0627\u06cc \u06a9\u0647 \u0645\u06cc \u062e\u0648\u0627\u0647\u06cc\u0645 \u062a\u06a9\u062b\u06cc\u0631 \u06a9\u0646\u06cc\u0645 DNA \u00a0\u0646\u0628\u0627\u0634\u062f \u0645\u062b\u0644\u0627 \u0628\u0631\u0627\u06cc \u0628\u0631\u0631\u0633\u06cc \u0639\u0641\u0648\u0646\u062a \u0628\u0627 \u0648\u06cc\u0631\u0648\u0633 \u0647\u0627\u06cc RNA \u00a0\u062f\u0627\u0631. \u0633\u0627\u062e\u062a \u06a9\u062a\u0627\u0628\u062e\u0627\u0646\u0647 \u0698\u0646\u06cc cDNA \u0628\u0647 \u0645\u0646\u0638\u0648\u0631 \u062d\u0630\u0641 \u0646\u0648\u0627\u062d\u06cc \u063a\u06cc\u0631 \u06a9\u0646\u0646\u062f\u0647 \u00a0\u0648 \u0639\u0646\u0627\u0635\u0631 \u062a\u0646\u0638\u06cc\u0645\u06cc \u0645\u0648\u062c\u0648\u062f \u062f\u0631 DNA \u0698\u0646\u0648\u0645\u06cc \u00a0\u0648 \u06a9\u0644\u0648\u0646 \u06a9\u0631\u062f\u0646 \u0648 \u0628\u06cc\u0627\u0646 \u0698\u0646 \u0647\u0627\u06cc \u06cc\u0648\u06a9\u0627\u0631\u06cc\u0648\u062a\u06cc \u062f\u0631 \u067e\u0631\u0648\u06a9\u0627\u0631\u06cc\u0648\u062a \u0647\u0627 \u0648 \u062f\u0631 \u0646\u0647\u0627\u06cc\u062a \u0645\u0647\u0645 \u062a\u0631\u06cc\u0646 \u06a9\u0627\u0631\u0628\u0631\u062f \u0622\u0646 \u0627\u06cc\u0646 \u0627\u0633\u062a \u06a9\u0647 \u0628\u0631\u0627\u06cc \u0628\u0631\u0631\u0633\u06cc \u0645\u06cc\u0632\u0627\u0646 \u0628\u06cc\u0627\u0646 \u0698\u0646 \u0647\u0627 \u0628\u0647 \u062a\u0639\u06cc\u06cc\u0646 \u0645\u0642\u062f\u0627\u0631 mRNA \u0646\u06cc\u0627\u0632 \u062f\u0627\u0631\u06cc\u0645 \u06a9\u0647 \u062f\u0631 \u0648\u0627\u0642\u0639 \u0645\u06cc \u062a\u0648\u0627\u0646 \u06af\u0641\u062a \u0627\u0648\u0644\u06cc\u0646 \u06af\u0627\u0645 \u0628\u0631\u0627\u06cc \u0627\u0646\u062c\u0627\u0645 RealTime PCR \u0633\u0646\u062a\u0632 cDNA(<strong>Complementry DNA<\/strong>) \u062a\u0648\u0633\u0637 RT-PCR \u00a0\u0645\u06cc \u0628\u0627\u0634\u062f \u0628\u0647 \u062f\u0644\u06cc\u0644 \u0627\u06cc\u0646\u06a9\u0647 \u0622\u0646\u0632\u06cc\u0645 DNA \u067e\u0644\u06cc\u0645\u0631\u0627\u0632\u06cc \u06a9\u0647 \u0628\u0631\u0627\u06cc \u0633\u0646\u062a\u0632 \u0631\u0634\u062a\u0647 \u0647\u0627\u06cc \u062c\u062f\u06cc\u062f \u062f\u0631 \u0627\u0646\u0648\u0627\u0639 <a href=\"http:\/\/darwino.ir\/pcr-technique\/\"><strong>PCR<\/strong> <\/a>\u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f \u0642\u0627\u062f\u0631 \u0628\u0647 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 RNA \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u0627\u0644\u06af\u0648 \u0646\u06cc\u0633\u062a. \u0627\u0633\u0627\u0633 \u0633\u0646\u062a\u0632 cDNA \u0628\u0631 \u0645\u0628\u0646\u0627\u06cc \u0622\u0646\u0632\u06cc\u0645 \u0646\u0633\u062e\u0647 \u0628\u0631\u062f\u0627\u0631 \u0645\u0639\u06a9\u0648\u0633 (Reverse transcriptase) \u0645\u06cc \u0628\u0627\u0634\u062f \u06a9\u0647 \u0642\u0627\u0628\u0644\u06cc\u062a \u0633\u0646\u062a\u0632 DNA \u062a\u06a9 \u0631\u0634\u062a\u0647 \u0627\u06cc \u0627\u0632 \u0631\u0648\u06cc \u062a\u0648\u0627\u0644\u06cc RNA \u062a\u06a9 \u0631\u0634\u062a\u0647 \u0627\u06cc \u0631\u0627 \u062f\u0627\u0631\u062f \u0648 \u0627\u0648\u0644\u06cc\u0646 \u0628\u0627\u0631 \u062f\u0631 \u0627\u0648\u0627\u06cc\u0644 \u062f\u0647\u0647 \u06f1\u06f9\u06f7\u06f0 \u0627\u0632 \u0631\u062a\u0631\u0648\u0648\u06cc\u0631\u0648\u0633 \u0647\u0627 \u062e\u0627\u0631\u062c \u0634\u062f \u06a9\u0647 \u062f\u0631 \u0648\u0627\u0642\u0639 \u06cc\u06a9 DNA \u067e\u0644\u06cc\u0645\u0631\u0627\u0632 \u0648\u0627\u0628\u0633\u062a\u0647 \u0628\u0647 RNA \u00a0\u0647\u0633\u062a \u06a9\u0647 \u0686\u0646\u062f\u06cc\u0646 \u0646\u0648\u0639 \u0627\u0632 \u0627\u06cc\u0646 \u0622\u0646\u0632\u06cc\u0645 \u0648\u062c\u0648\u062f \u062f\u0627\u0631\u062f:AMV \u060c MMLV \u060cHIV-1 \u00a0\u0648 \u0622\u0646\u0632\u06cc\u0645 \u0646\u0633\u062e\u0647 \u0628\u0631\u062f\u0627\u0631 \u0645\u0639\u06a9\u0648\u0633 \u062a\u0644\u0648\u0645\u0631\u0627\u0632\u06cc.<\/p>\n<h3><strong>\u0627\u0632 \u062f\u06cc\u06af\u0631 \u0645\u0648\u0627\u062f \u0645\u0647\u0645 \u0628\u0631\u0627\u06cc \u0633\u0646\u062a\u0632 cDNA\u00a0\u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627 \u0647\u0633\u062a\u0646\u062f \u06a9\u0647 \u0628\u0631\u0627\u06cc \u0627\u06cc\u0646 \u06a9\u0627\u0631 \u06f3 \u0646\u0648\u0639 \u067e\u0631\u0627\u06cc\u0645\u0631 \u0648\u062c\u0648\u062f \u062f\u0627\u0631\u062f:<\/strong><\/h3>\n<p><strong>\u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc <\/strong><strong>Random Hexamer<\/strong><strong>:<\/strong> \u062a\u0648\u0627\u0644\u06cc \u0647\u0627\u06cc \u06a9\u0648\u0686\u06a9 \u06f6 \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f\u06cc \u06a9\u0647 \u0628\u0647 \u0635\u0648\u0631\u062a \u062a\u0635\u0627\u062f\u0641\u06cc \u0628\u0647 \u0647\u0631 \u062c\u0627\u06cc\u06cc \u0627\u0632 RNA \u0627\u0645\u06a9\u0627\u0646 \u0627\u062a\u0635\u0627\u0644 \u062f\u0627\u0631\u0646\u062f. \u0627\u06cc\u0646 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627 \u0632\u0645\u0627\u0646\u06cc \u06a9\u0647 \u0628\u062e\u0648\u0627\u0647\u06cc\u0645 \u0628\u06cc\u0627\u0646 \u062a\u0645\u0627\u0645 \u0698\u0646 \u0647\u0627 \u0631\u0627 \u062a\u062d\u062a \u0647\u0631 \u0634\u0631\u0627\u06cc\u0637\u06cc \u0628\u0627 \u0647\u0631 \u0698\u0646 \u06a9\u0646\u062a\u0631\u0644\u06cc \u062d\u062a\u06cc rRNA 18s \u00a0\u0628\u0631\u0631\u0633\u06cc \u06a9\u0646\u06cc\u0645 \u06a9\u0627\u0631\u0628\u0631\u062f \u062f\u0627\u0631\u062f.<\/p>\n<p><strong>\u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc <\/strong><strong>OligodT<\/strong><strong>:<\/strong> \u0627\u06cc\u0646 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627 \u0628\u0647 \u0627\u0646\u062a\u0647\u0627\u06cc mRNA \u00a0\u062f\u0627\u0631\u0627\u06cc \u062a\u0648\u0627\u0644\u06cc polyA \u00a0\u0645\u062a\u0635\u0644 \u0645\u06cc \u0634\u0648\u0646\u062f.<\/p>\n<p><strong>\u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc \u0627\u062e\u062a\u0635\u0627\u0635\u06cc:<\/strong> \u0627\u06cc\u0646 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627 \u0628\u0631\u0627\u06cc \u0646\u0642\u0627\u0637 \u00a0\u0634\u0646\u0627\u062e\u062a\u0647 \u0634\u062f\u0647 \u0627\u06cc \u0627\u0632 mRNA \u0647\u0627 \u0628\u0627 \u062a\u0648\u0627\u0644\u06cc \u0645\u0634\u062e\u0635 \u0637\u0631\u0627\u062d\u06cc \u0645\u06cc \u0634\u0648\u0646\u062f \u0648 \u062f\u0631 \u0648\u0627\u0642\u0639 \u0628\u0627\u0639\u062b \u0628\u0647\u06cc\u0646\u0647 \u0633\u0627\u0632\u06cc \u062a\u0648\u0627\u0644\u06cc \u0645\u0648\u0631\u062f \u0646\u0638\u0631 \u0645\u06cc \u06af\u0631\u062f\u0646\u062f \u0648 \u062f\u0631 \u06a9\u0644\u0648\u0646\u06cc\u0646\u06af \u06a9\u0627\u0631\u0628\u0631\u062f \u062f\u0627\u0631\u0646\u062f. \u0645\u0648\u0627\u062f \u062f\u06cc\u06af\u0631\u06cc \u06a9\u0647 \u062f\u0631 \u0633\u0646\u062a\u0632 cDNA \u0646\u0642\u0634 \u062f\u0627\u0631\u0646\u062f \u0634\u0627\u0645\u0644 \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f\u0647\u0627( dNTP\u0647\u0627)\u060c \u0628\u0627\u0641\u0631 \u0622\u0646\u0632\u06cc\u0645 RT\u060c \u0622\u0646\u0632\u06cc\u0645 \u0631\u06cc\u0628\u0648\u0646\u0648\u06a9\u0644\u0626\u0627\u0632 \u0648RNase H \u00a0\u06a9\u0647 \u0648\u0638\u06cc\u0641\u0647 \u06cc \u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u062f\u0648 \u0631\u0634\u062a\u0647 \u06cc RNA \u00a0\u0648 cDNA \u00a0\u0633\u0646\u062a\u0632 \u0634\u062f\u0647 \u0627\u0632 \u0647\u0645 \u0631\u0627 \u062f\u0627\u0631\u062f\u060c \u0645\u06cc \u0628\u0627\u0634\u062f . \u06a9\u0647 \u0645\u0639\u0645\u0648\u0644\u0627 \u0647\u0645\u0647 \u0627\u06cc\u0646 \u0645\u0648\u0627\u062f \u0628\u0647 \u0635\u0648\u0631\u062a \u0645\u062e\u0644\u0648\u0637\u06cc \u062f\u0631 \u06a9\u06cc\u062a \u0647\u0627\u06cc \u0633\u0646\u062a\u0632 cDNA \u062f\u0631 \u0648\u06cc\u0627\u0644\u06cc \u062a\u062d\u062a \u0639\u0646\u0648\u0627\u0646 Master Mix \u0645\u0648\u062c\u0648\u062f \u0645\u06cc \u0628\u0627\u0634\u062f \u0648 \u0646\u06cc\u0627\u0632\u06cc \u0628\u0647 \u0627\u0641\u0632\u0648\u062f\u0646 \u0622\u0646 \u0647\u0627 \u0628\u0647 \u0635\u0648\u0631\u062a \u062c\u062f\u0627\u06af\u0627\u0646\u0647 \u0646\u06cc\u0633\u062a.<\/p>\n<p>\u0628\u0631\u062e\u06cc \u06a9\u06cc\u062a \u0647\u0627 \u0628\u0647 \u0635\u0648\u0631\u062a <strong>One step<\/strong> \u0648 \u0628\u0631\u062e\u06cc <strong>Two step<\/strong> \u0645\u06cc \u00a0\u0628\u0627\u0634\u0646\u062f. \u062f\u0631 \u062d\u0627\u0644\u062a \u00a0Two step \u0627\u0628\u062a\u062f\u0627 \u0633\u0646\u062a\u0632 cDNA \u00a0\u062f\u0631 \u06cc\u06a9 \u0648\u06cc\u0627\u0644 \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u0634\u0648\u062f \u0648 \u0633\u067e\u0633 PCR \u0645\u0639\u0645\u0648\u0644\u06cc \u062f\u0631 \u062c\u0647\u062a <a href=\"http:\/\/darwino.ir\/realtime-pcr\/\"><strong>\u0628\u0631\u0631\u0633\u06cc \u0628\u06cc\u0627\u0646 \u0698\u0646<\/strong><\/a> cDNA \u0633\u0646\u062a\u0632\u0634\u062f\u0647 \u062f\u0631 \u0648\u06cc\u0627\u0644\u06cc \u062f\u06cc\u06af\u0631 \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u0634\u0648\u062f. \u0627\u06cc\u0646 \u06a9\u06cc\u062a \u0647\u0627 \u062f\u0642\u062a \u0628\u06cc\u0634\u062a\u0631\u06cc \u062f\u0627\u0631\u0646\u062f\u00a0 \u0648 Trouble shooting \u00a0\u0647\u0631 \u0645\u0631\u062d\u0644\u0647 \u0622\u0633\u0627\u0646 \u062a\u0631 \u0645\u06cc \u0628\u0627\u0634\u062f.<\/p>\n<p>\u062f\u0631 \u062d\u0627\u0644\u062a <strong>One step<\/strong> \u0647\u0645\u0647 \u06cc \u0645\u0631\u0627\u062d\u0644 \u0633\u0646\u062a\u0632 cDNA \u062a\u0627 \u0628\u06cc\u0627\u0646 \u0698\u0646 \u062f\u0631 \u06cc\u06a9 \u0648\u06cc\u0627\u0644 \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u06af\u06cc\u0631\u062f \u0648 \u0628\u0631\u0627\u06cc \u0627\u0641\u0631\u0627\u062f\u06cc \u06a9\u0647 \u062a\u0633\u0644\u0637 \u0632\u06cc\u0627\u062f\u06cc \u062f\u0627\u0631\u0646\u062f \u0645\u0646\u0627\u0633\u0628 \u062a\u0631\u0627\u0633\u062a. \u0628\u0631\u0627\u06cc \u0627\u0646\u062c\u0627\u0645 \u0627\u06cc\u0646 \u062a\u06a9\u0646\u06cc\u06a9 \u0627\u0628\u062a\u062f\u0627 <a href=\"http:\/\/darwino.ir\/rna-extraction-with-triazole\/\"><strong>\u0627\u0633\u062a\u062e\u0631\u0627\u062c RNA<\/strong> <\/a>\u0645\u0648\u062c\u0648\u062f \u062f\u0631 \u0646\u0645\u0648\u0646\u0647 \u0645\u0648\u0631\u062f \u0646\u0638\u0631 \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u0634\u0648\u062f. RNA \u0627\u0633\u062a\u062e\u0631\u0627\u062c \u0634\u062f\u0647 \u062c\u0647\u062a \u0633\u0646\u062a\u0632 cDNA \u0628\u0627\u06cc\u062f \u06a9\u06cc\u0641\u06cc\u062a \u0648 \u063a\u0644\u0638\u062a \u0645\u0646\u0627\u0633\u0628\u06cc \u062f\u0627\u0634\u062a\u0647 \u0628\u0627\u0634\u062f \u0686\u0648\u0646 \u0686\u06cc\u0632\u06cc \u0645\u0639\u0627\u062f\u0644 \u06f1\u03bcg \u06cc\u0627 \u0647\u0645\u0627\u0646 \u06f1\u06f0\u06f0\u06f0ng \u0627\u0632 RNA \u062f\u0631 \u0647\u0631 \u0628\u0627\u0631 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u062c\u0647\u062a \u0633\u0646\u062a\u0632 \u0645\u0648\u0631\u062f \u0646\u06cc\u0627\u0632 \u0627\u0633\u062a. \u0633\u067e\u0633 RNA \u00a0\u0645\u0648\u0631\u062f \u0646\u0638\u0631 \u0631\u0627 \u0628\u0627 \u0645\u0648\u0627\u062f\u06cc \u06a9\u0647 \u062f\u0631 \u0628\u0627\u0644\u0627 \u06af\u0641\u062a\u0647 \u0634\u062f \u0628\u0631 \u0631\u0648\u06cc \u06cc\u062e \u0645\u062e\u0644\u0648\u0637 \u06a9\u0631\u062f\u06cc\u0645 \u0648\u06cc\u0627\u0644 \u0631\u0627 \u0628\u0647 \u062f\u0633\u062a\u06af\u0627\u0647 \u00a0\u062a\u0631\u0645\u0648\u0633\u0627\u06cc\u06a9\u0644\u0631 \u0645\u0646\u062a\u0642\u0644 \u0645\u06cc \u06a9\u0646\u06cc\u0645 \u062a\u0627 \u062f\u0645\u0627\u0647\u0627\u06cc \u0644\u0627\u0632\u0645 \u0631\u0627 \u0628\u0627 \u0628\u0631\u0646\u0627\u0645\u0647 \u0632\u0645\u0627\u0646\u06cc \u06a9\u0647 \u0628\u0633\u062a\u0647 \u0628\u0647 \u06a9\u06cc\u062a \u0645\u062a\u0641\u0627\u0648\u062a \u0627\u0633\u062a \u0627\u0639\u0645\u0627\u0644 \u06a9\u0646\u06cc\u0645.<\/p>\n<h3><strong>\u0645\u0631\u0627\u062d\u0644 \u0645\u0634\u062a\u0631\u06a9 \u062f\u0631 \u06a9\u06cc\u062a \u0647\u0627\u06cc \u0645\u062e\u062a\u0644\u0641:<\/strong><\/h3>\n<p>\u0627\u0641\u0632\u0627\u06cc\u0634 \u062f\u0645\u0627 \u062a\u0627 \u06f6\u06f5 \u062f\u0631\u062c\u0647 \u0628\u0647 \u0645\u062f\u062a \u06f5 \u062f\u0642\u06cc\u0642\u0647\u00a0 \u0628\u0631\u0627\u06cc \u0628\u0627\u0632 \u0634\u062f\u0646 \u067e\u06cc\u0686 \u0648 \u062a\u0627\u0628 \u0647\u0627 \u0648 \u0633\u0627\u062e\u062a\u0627\u0631 \u0647\u0627\u06cc \u062b\u0627\u0646\u0648\u06cc\u0647 RNA \u062f\u0645\u0627\u06cc \u062d\u062f\u0648\u062f\u06f4\u06f0 (\u06f4\u06f2-\u06f3\u06f7) \u062f\u0631\u062c\u0647 \u0628\u0647 \u0645\u062f\u062a \u06cc\u06a9 \u0633\u0627\u0639\u062a \u0628\u0647 \u0645\u0646\u0638\u0648\u0631 \u0641\u0639\u0627\u0644 \u0634\u062f\u0646 \u0622\u0646\u0632\u06cc\u0645 RT \u00a0\u06a9\u0647 \u0627\u06cc\u0646 \u062f\u0645\u0627 \u0628\u0633\u062a\u0647 \u0628\u0647 \u0646\u0648\u0639 \u0622\u0646\u0632\u06cc\u0645 RT \u0645\u06cc \u062a\u0648\u0627\u0646\u062f \u062a\u063a\u06cc\u06cc\u0631 \u06a9\u0646\u062f. \u062f\u0645\u0627\u06cc \u06f8\u06f0-\u06f7\u06f0 \u062f\u0631 \u062d\u062f\u0648\u062f \u06f5 \u062f\u0642\u06cc\u0642\u0647 \u0628\u0631\u0627\u06cc \u063a\u06cc\u0631 \u0641\u0639\u0627\u0644 \u06a9\u0631\u062f\u0646 \u0622\u0646\u0632\u06cc\u0645 RT cDNA \u0633\u0646\u062a\u0632 \u0634\u062f\u0647 \u0631\u0627 \u0645\u06cc \u062a\u0648\u0627\u0646 \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f2\u06f0- \u062f\u0631\u062c\u0647 \u0628\u0647 \u0645\u062f\u062a \u0637\u0648\u0644\u0627\u0646\u06cc \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u06a9\u0631\u062f \u0648 \u062f\u06cc\u06af\u0631 \u0627\u0633\u062a\u0631\u0633 \u062a\u062e\u0631\u06cc\u0628 \u0622\u0646 \u0628\u0627 \u0622\u0646\u0632\u06cc\u0645 RNase \u06a9\u0647 \u062f\u0631 \u0645\u062d\u06cc\u0637 \u0641\u0631\u0627\u0648\u0627\u0646 \u0627\u0633\u062a \u0631\u0627 \u0646\u06cc\u0632 \u0646\u062f\u0627\u0634\u062a.<\/p>\n<p>\u0628\u0631\u0627\u06cc \u0628\u0631\u0631\u0633\u06cc \u06a9\u06cc\u0641\u06cc\u062a cDNA \u0628\u0627\u06cc\u062f \u0628\u0631\u0627\u06cc \u06cc\u06a9 \u0698\u0646 <strong>House keeping<\/strong>\u00a0 \u06a9\u0647 \u0645\u06cc\u0632\u0627\u0646 \u0628\u06cc\u0627\u0646 \u0622\u0646 \u062f\u0631 \u0647\u0645\u0647 \u0633\u0644\u0648\u0644 \u0647\u0627 \u062a\u062d\u062a \u0647\u0631 \u0634\u0631\u0627\u06cc\u0637\u06cc \u06cc\u06a9\u0633\u0627\u0646 \u0627\u0633\u062a \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u0645\u0628\u0646\u0627 \u0648 \u0631\u0641\u0631\u0646\u0633\u00a0 \u06cc\u06a9 PCR \u0645\u0639\u0645\u0648\u0644\u06cc \u0628\u06af\u0630\u0627\u0631\u06cc\u0645 \u0648 \u0646\u062a\u06cc\u062c\u0647 \u0631\u0627 \u0631\u0648\u06cc <a href=\"http:\/\/darwino.ir\/agarose_gel_electrophoresis\/\"><strong>\u0698\u0644 \u0622\u06af\u0627\u0631\u0632<\/strong><\/a> \u0645\u0634\u0627\u0647\u062f\u0647 \u06a9\u0646\u06cc\u0645 \u0686\u0646\u0627\u0646\u0686\u0647 \u0628\u0627\u0646\u062f SHARP \u0648 \u062f\u0631 \u062c\u0627\u06cc\u06af\u0627\u0647 \u062f\u0631\u0633\u062a\u06cc \u0627\u0632 \u0644\u062d\u0627\u0638 \u0637\u0648\u0644 \u0628\u0648\u062f \u0646\u062a\u06cc\u062c\u0647 \u0645\u06cc \u06af\u06cc\u0631\u06cc\u0645 \u06a9\u06cc\u0641\u06cc\u062a cDNA \u0645\u0646\u0627\u0633\u0628 \u0627\u0633\u062a \u0648 \u0628\u0627 \u062e\u06cc\u0627\u0644 \u0631\u0627\u062d\u062a \u0645\u06cc \u062a\u0648\u0627\u0646\u06cc\u0645 \u0628\u0647 \u0633\u0631\u0627\u063a \u0627\u0646\u062c\u0627\u0645 \u0645\u0631\u0627\u062d\u0644 \u0628\u0639\u062f\u06cc RealTime PCR \u062c\u0647\u062a \u0628\u0631\u0631\u0633\u06cc \u0628\u06cc\u0627\u0646 \u0698\u0646 \u0647\u0627\u00a0 \u0631\u0641\u062a.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u270d \u00a0RT-PCR \u0686\u0647 \u062a\u06a9\u0646\u06cc\u06a9\u06cc \u0627\u0633\u062a \u0648 \u062f\u0631 \u0686\u0647 \u0645\u0648\u0627\u0631\u062f\u06cc \u06a9\u0627\u0631\u0628\u0631\u062f [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":2896,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[30],"tags":[3284,1586,1584,1587,564,779,882,1589,1585,1590,531,1588],"blog-category":[3070,3071],"darwinoplus-category":[],"class_list":{"0":"post-2928","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","6":"hentry","7":"category-blog","8":"tag-complementry_dna","9":"tag-_rt_pcr","10":"tag-_rt_pcr___cdna","11":"tag-_transcription_pcr","12":"tag-___","15":"tag-___rt_pcr___cdna","16":"tag-_housekeeping","17":"tag-_pdf_-rt_pcr___cdna","18":"tag-__real_time_pcr","19":"tag-____rt_pcr___cdna","20":"blog-category-genetic","21":"blog-category-molecular"},"_links":{"self":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/2928","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/comments?post=2928"}],"version-history":[{"count":21,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/2928\/revisions"}],"predecessor-version":[{"id":23182,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/2928\/revisions\/23182"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media\/2896"}],"wp:attachment":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media?parent=2928"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/categories?post=2928"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/tags?post=2928"},{"taxonomy":"blog-category","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/blog-category?post=2928"},{"taxonomy":"darwinoplus-category","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/darwinoplus-category?post=2928"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}