{"id":13450,"date":"2019-07-31T03:07:58","date_gmt":"2019-07-31T03:07:58","guid":{"rendered":"http:\/\/darwino.ir\/blogs\/?p=13450"},"modified":"2020-03-28T12:10:23","modified_gmt":"2020-03-28T08:40:23","slug":"real-time-pcr-technique-checking-controls-and-sample","status":"publish","type":"post","link":"https:\/\/darwino.ir\/blogs\/real-time-pcr-technique-checking-controls-and-sample\/","title":{"rendered":"\u062a\u06a9\u06cc\u0646\u06a9 RealTime PCR -\u0628\u062e\u0634 \u0634\u0634\u0645(\u0627\u0634\u06a9\u0627\u0644\u0627\u062a \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631-\u06f2)"},"content":{"rendered":"<h2><strong>\u270d <\/strong><strong>\u0628\u0631\u0631\u0633\u06cc \u06a9\u0646\u062a\u0631\u0644 \u0647\u0627\u060c \u0646\u0645\u0648\u0646\u0647 \u0627\u0635\u0644\u06cc \u0648 \u0622\u0646\u0627\u0644\u06cc\u0632 \u0645\u0646\u062d\u0646\u06cc \u0630\u0648\u0628 \u062c\u0647\u062a \u0631\u0641\u0639 \u0627\u0634\u06a9\u0627\u0644\u0627\u062a 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\u0633\u06cc\u06a9\u0644 \u0628\u06cc\u0646 \u0646\u0645\u0648\u0646\u0647 \u06cc \u0627\u0635\u0644\u06cc \u0648 \u0646\u0645\u0648\u0646\u0647 \u06cc \u06a9\u0646\u062a\u0631\u0644 \u0628\u062f\u0648\u0646 \u0627\u0644\u06af\u0648 \u0645\u0634\u0627\u0647\u062f\u0647 \u0634\u0648\u062f \u0648 \u0627\u06af\u0631 \u0627\u06cc\u0646 \u0627\u062e\u062a\u0644\u0627\u0641 \u0645\u0634\u0627\u0647\u062f\u0647 \u0646\u0634\u062f\u060c \u0646\u0634\u0627\u0646 \u0627\u0632 \u0622\u0644\u0648\u062f\u06af\u06cc \u0645\u06cc \u0628\u0627\u0634\u062f.<\/p>\n<p><strong>\u0622\u0646\u0627\u0644\u06cc\u0632 \u0645\u0646\u062d\u0646\u06cc \u0630\u0648\u0628:<\/strong> \u062f\u0631 \u0631\u0648\u0634 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u0631\u0646\u06af \u0647\u0627\u06cc \u0622\u0632\u0627\u062f\u060c \u0628\u0631\u0627\u06cc \u0628\u0631\u0631\u0633\u06cc \u0639\u0645\u0644\u06a9\u0631\u062f \u067e\u0631\u0627\u06cc\u0645\u0631 \u0647\u0627\u06cc \u0637\u0631\u0627\u062d\u06cc \u0634\u062f\u0647 \u0648 \u0627\u0637\u0645\u06cc\u0646\u0627\u0646 \u0627\u0632 \u0627\u06cc\u0646 \u06a9\u0647 \u067e\u0631\u0627\u06cc\u0645\u0631 \u0641\u0642\u0637 \u0628\u0647 \u0647\u062f\u0641 \u0645\u0648\u0631\u062f \u0646\u0638\u0631 \u0645\u062a\u0635\u0644 \u0634\u062f\u0647 \u0648 \u062a\u06a9\u062b\u06cc\u0631 \u0628\u0647 \u062f\u0631\u0633\u062a\u06cc \u0648 \u0628\u0627 \u0627\u062e\u062a\u0635\u0627\u0635\u06cc\u062a \u06a9\u0627\u0641\u06cc \u0627\u0646\u062c\u0627\u0645 \u0634\u062f\u0647 \u0627\u0633\u062a\u060c \u0645\u06cc \u062a\u0648\u0627\u0646\u06cc\u062f \u0628\u0627 \u0628\u0631\u0631\u0633\u06cc \u0622\u0646\u0627\u0644\u06cc\u0632 \u0630\u0648\u0628 \u0646\u062a\u0627\u06cc\u062c \u0631\u0627 \u062a\u0641\u0633\u06cc\u0631 \u06a9\u0646\u06cc\u062f\u060c \u062f\u0631 \u0648\u0627\u0642\u0639 \u0648\u062c\u0648\u062f \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0641\u0642\u0637 \u0645\u06cc \u062a\u0648\u0627\u0646\u062f \u0646\u0634\u0627\u0646 \u0627\u0632 \u06cc\u06a9 \u062a\u06a9\u062b\u06cc\u0631 \u0628\u0627\u0634\u062f \u0648 \u0648\u0627\u0642\u0639\u06cc \u0628\u0648\u062f\u0646 \u06cc\u0627 \u0646\u0628\u0648\u062f\u0646 \u0627\u062e\u062a\u0635\u0627\u0635\u06cc\u062a \u0622\u0646 \u0628\u0627\u06cc\u062f \u062a\u0623\u06cc\u06cc\u062f \u0634\u0648\u062f\u060c \u06a9\u0647 \u0645\u06cc \u062a\u0648\u0627\u0646 \u0627\u0632 \u0645\u0646\u062d\u0646\u06cc \u0622\u0646\u0627\u0644\u06cc\u0632 \u0630\u0648\u0628 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u06a9\u0631\u062f. \u0647\u0645\u0627\u0646 \u0637\u0648\u0631 \u06a9\u0647 \u06af\u0641\u062a\u0647 \u0634\u062f\u060c \u0627\u0641\u062a \u0641\u0644\u0648\u0631\u0633\u0646\u0633 \u0628\u0631 \u0631\u0648\u06cc \u0645\u0646\u062d\u0646\u06cc \u0646\u0645\u0648\u062f\u0627\u0631 \u0645\u0646\u062d\u0646\u06cc \u0630\u0648\u0628 \u0628\u0647 \u0635\u0648\u0631\u062a \u0642\u0644\u0647 \u062f\u06cc\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f \u06a9\u0647 \u062f\u0631 \u0648\u0627\u0642\u0639 \u0628\u0631\u0627\u06cc \u0645\u0634\u0627\u0647\u062f\u0647 \u06cc \u0631\u0627\u062d\u062a \u062a\u0631 \u0646\u0645\u0648\u062f\u0627\u0631 \u0627\u06cc\u0646 \u0686\u0646\u06cc\u0646 \u0646\u0645\u0627\u06cc\u0634 \u062f\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f .<\/p>\n<p>\u0628\u0631\u0627\u06cc \u062f\u0631\u06a9 \u0628\u0647\u062a\u0631 \u06cc\u06a9 \u0645\u062b\u0627\u0644 \u0628\u0631\u0627\u06cc\u062a\u0627\u0646 \u062e\u0648\u0627\u0647\u06cc\u0645 \u06af\u0641\u062a\u060c \u0627\u06af\u0631 \u0627\u0646\u062f\u0627\u0632\u0647 \u06cc \u0642\u0637\u0639\u0647 \u06f1\u06f3\u06f0 \u062c\u0641\u062a \u0628\u0627\u0632 \u0648 \u062f\u0645\u0627\u06cc Tm \u0645\u062d\u0635\u0648\u0644 PCR \u0645\u0627 \u06f8\u06f0 \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc \u06af\u0631\u0627\u062f \u0628\u0627\u0634\u062f \u0627\u06cc\u0646 \u0627\u0646\u062a\u0638\u0627\u0631 \u0631\u0627 \u062f\u0627\u0631\u06cc\u0645 \u06a9\u0647 \u062f\u0631 \u0645\u0646\u062d\u0646\u06cc \u0622\u0646\u0627\u0644\u06cc\u0632 \u0630\u0648\u0628 \u0641\u0642\u0637 \u06cc\u06a9 \u067e\u06cc\u06a9 \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u062f\u0631 \u0645\u062d\u0644 \u062f\u0645\u0627\u06cc Tm \u06a9\u0647 \u06f8\u06f0 \u062f\u0631\u062c\u0647 \u0627\u0633\u062a \u062f\u0627\u0634\u062a\u0647 \u0628\u0627\u0634\u06cc\u0645. \u0627\u06af\u0631 \u062f\u0648 \u067e\u06cc\u06a9 \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u062f\u0631 \u0645\u0646\u062d\u0646\u06cc \u0622\u0646\u0627\u0644\u06cc\u0632 \u0630\u0648\u0628 \u0645\u0634\u0627\u0647\u062f\u0647 \u0634\u062f\u060c \u0646\u0634\u0627\u0646 \u0627\u0632 \u062a\u06a9\u062b\u06cc\u0631 \u062f\u0648 \u0642\u0637\u0639\u0647 \u06cc \u062a\u0648\u0644\u06cc\u062f\u06cc \u0645\u06cc \u0628\u0627\u0634\u062f \u0648 \u067e\u0631\u0627\u06cc\u0645\u0631 \u0637\u0631\u0627\u062d\u06cc \u0634\u062f\u0647 \u0628\u0647 \u0635\u0648\u0631\u062a \u063a\u06cc\u0631 \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u0628\u0647 \u06cc\u06a9 \u062a\u0648\u0627\u0644\u06cc \u062f\u06cc\u06af\u0631 \u0645\u062a\u0635\u0644 \u0634\u062f\u0647 \u0627\u0633\u062a. \u0627\u062d\u062a\u0645\u0627\u0644 \u0632\u06cc\u0627\u062f \u0627\u06af\u0631 \u067e\u06cc\u06a9 \u062f\u0648\u0645 \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f7\u06f5 \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc \u06af\u0631\u0627\u062f \u0645\u0634\u0627\u0647\u062f\u0647 \u0634\u062f\u060c \u0646\u0634\u0627\u0646 \u0627\u0632 \u062a\u0634\u06a9\u06cc\u0644 \u062f\u0627\u06cc\u0645\u0631 \u067e\u0631\u0627\u06cc\u0645\u0631 \u0648 \u063a\u06cc\u0631 \u0628\u0647\u06cc\u0646\u0647 \u0628\u0648\u062f\u0646 \u0648\u0627\u06a9\u0646\u0634 \u062f\u0627\u0631\u062f. \u062a\u0648\u062c\u0647 \u062f\u0627\u0634\u062a\u0647 \u0628\u0627\u0634\u06cc\u062f \u06a9\u0647 \u062f\u0631 \u0647\u0646\u06af\u0627\u0645 \u0637\u0631\u0627\u062d\u06cc \u067e\u0631\u0627\u06cc\u0645\u0631\u060c \u0646\u0631\u0645 \u0627\u0641\u0632\u0627\u0631 \u0628\u0647 \u0634\u0645\u0627 \u062f\u0645\u0627\u06cc \u0630\u0648\u0628 \u0642\u0637\u0639\u0647 \u06cc \u062a\u0648\u0644\u06cc\u062f\u06cc \u0631\u0627 \u062e\u0648\u0627\u0647\u062f \u062f\u0627\u062f \u0648 \u0634\u0645\u0627 \u0645\u06cc \u062a\u0648\u0627\u0646\u06cc\u062f \u0628\u0631 \u0627\u0633\u0627\u0633 \u0622\u0646 \u0645\u0646\u062d\u0646\u06cc \u0622\u0646\u0627\u0644\u06cc\u0632 \u0630\u0648\u0628 \u062e\u0648\u062f \u0631\u0627 \u0628\u0631\u0631\u0633\u06cc \u0648 \u0622\u0646\u0627\u0644\u06cc\u0632 \u06a9\u0646\u06cc\u062f.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/>\u00a0<a href=\"http:\/\/darwino.ir\/realtime-pcr\/\">\u062a\u06a9\u0646\u06cc\u06a9\/\u0631\u0648\u0634 RealTime PCR \u2013 \u0628\u062e\u0634 \u0627\u0648\u0644 (\u0627\u0646\u0648\u0627\u0639 \u0631\u0648\u0634\u0647\u0627\u06cc \u0622\u0632\u0645\u0627\u06cc\u0634)<\/a><\/strong><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><a href=\"http:\/\/darwino.ir\/real-time-pcr-section-2\/\"><strong>\u062a\u06a9\u0646\u06cc\u06a9 Real-Time PCR \u2013 \u0628\u062e\u0634 \u062f\u0648\u0645 (\u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a)<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><a href=\"http:\/\/darwino.ir\/realtime-pcr-technique-part-3\/\"><strong>\u062a\u06a9\u0646\u06cc\u06a9 RealTime PCR \u2013 \u0628\u062e\u0634 \u0633\u0648\u0645(\u0645\u0631\u0627\u062d\u0644 \u0627\u0646\u062c\u0627\u0645 \u0648\u0627\u06a9\u0646\u0634)<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><a href=\"http:\/\/darwino.ir\/real-time-pcr-part-iv\/\"><strong>\u062a\u06a9\u0646\u06cc\u06a9 Real time PCR \u2013 \u0628\u062e\u0634 \u0686\u0647\u0627\u0631\u0645(\u0686\u0631\u062e\u0647 \u0627\u0646\u062c\u0627\u0645 \u0648\u0627\u06a9\u0646\u0634)<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><a href=\"http:\/\/darwino.ir\/realtime-pcr-reproduction-curves\/\">\u062a\u06a9\u06cc\u0646\u06a9 RealTime PCR &#8211; \u0628\u062e\u0634 \u067e\u0646\u062c\u0645(\u0627\u0634\u06a9\u0627\u0644\u0627\u062a \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 -\u06f1)<\/a><\/strong><a href=\"http:\/\/darwino.ir\/realtime-pcr\/\"><strong><br \/>\n<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><strong>\u062a\u06a9\u06cc\u0646\u06a9 RealTime PCR -\u0628\u062e\u0634 \u0634\u0634\u0645(\u0627\u0634\u06a9\u0627\u0644\u0627\u062a \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631-\u06f2)<\/strong><\/h4>\n","protected":false},"excerpt":{"rendered":"<p>\u270d \u0628\u0631\u0631\u0633\u06cc \u06a9\u0646\u062a\u0631\u0644 \u0647\u0627\u060c \u0646\u0645\u0648\u0646\u0647 \u0627\u0635\u0644\u06cc \u0648 \u0622\u0646\u0627\u0644\u06cc\u0632 \u0645\u0646\u062d\u0646\u06cc \u0630\u0648\u0628 [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":13452,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[30],"tags":[1374,807,779,1373,808,769,1372,809],"blog-category":[3070,3071],"darwinoplus-category":[],"class_list":["post-13450","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog","tag-_pdf________","tag-_real_time_pcr","tag-___","tag-_______","tag-____pcr","tag-____pcr_-_","tag-______-_","tag-__pcr","blog-category-genetic","blog-category-molecular"],"_links":{"self":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/13450","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/comments?post=13450"}],"version-history":[{"count":14,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/13450\/revisions"}],"predecessor-version":[{"id":21485,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/13450\/revisions\/21485"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media\/13452"}],"wp:attachment":[{"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media?parent=13450"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/categories?post=13450"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/tags?post=13450"},{"taxonomy":"blog-category","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/blog-category?post=13450"},{"taxonomy":"darwinoplus-category","embeddable":true,"href":"https:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/darwinoplus-category?post=13450"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}