{"id":4342,"date":"2019-07-06T04:08:11","date_gmt":"2019-07-06T04:08:11","guid":{"rendered":"http:\/\/darwino.ir\/blogs\/?p=4342"},"modified":"2020-05-01T14:11:40","modified_gmt":"2020-05-01T10:41:40","slug":"isolation-and-culture-of-mouse-embryo-fibroblasts","status":"publish","type":"post","link":"http:\/\/darwino.ir\/blogs\/isolation-and-culture-of-mouse-embryo-fibroblasts\/","title":{"rendered":"\u062a\u06a9\u0646\u06cc\u06a9 \u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u0648 \u06a9\u0634\u062a \u0641\u06cc\u0628\u0631\u0648\u0628\u0644\u0627\u0633\u062a \u062c\u0646\u06cc\u0646\u06cc \u0645\u0648\u0634"},"content":{"rendered":"<h2><strong>\u270d\u00a0<\/strong><strong>\u06a9\u0627\u0631\u0628\u0631\u062f \u062a\u06a9\u0646\u06cc\u06a9 \u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u0648 \u06a9\u0634\u062a \u0641\u06cc\u0628\u0631\u0648\u0628\u0644\u0627\u0633\u062a \u062c\u0646\u06cc\u0646\u06cc \u0645\u0648\u0634 \u062f\u0631 \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647 \u0647\u0627\u06cc \u0633\u0644\u0648\u0644\u06cc<\/strong><\/h2>\n<p>\u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u0648 \u06a9\u0634\u062a \u0641\u06cc\u0628\u0631\u0648\u0628\u0644\u0627\u0633\u062a \u062c\u0646\u06cc\u0646\u06cc \u0645\u0648\u0634\u06cc \u06cc\u06a9\u06cc \u0627\u0632 \u067e\u0627\u06cc\u0647 \u0627\u06cc \u062a\u0631\u06cc\u0646 \u0648 \u0627\u0633\u0627\u0633\u06cc \u062a\u0631\u06cc\u0646 \u0628\u062e\u0634 \u0647\u0627\u06cc \u0622\u0632\u0645\u0627\u06cc\u0634\u0627\u062a \u06a9\u0634\u062a \u0633\u0644\u0648\u0644\u06cc \u0627\u0633\u062a. \u0622\u0645\u0627\u062f\u0647 \u0633\u0627\u0632\u06cc \u0641\u06cc\u0628\u0631\u0648\u0628\u0644\u0627\u0633\u062a \u062c\u0646\u06cc\u0646\u06cc \u0645\u0648\u0634\u06cc \u0645\u0646\u0627\u0633\u0628 \u0628\u0631\u0627\u06cc \u06a9\u0634\u062a \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0628\u0646\u06cc\u0627\u062f\u06cc \u062c\u0646\u06cc\u0646 \u0627\u0646\u0633\u0627\u0646\u06cc (Human \u00a0Embryonic Stem Cell) \u0648 \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0628\u0646\u06cc\u0627\u062f\u06cc \u067e\u0631\u062a\u0648\u0627\u0646 \u0627\u0644\u0642\u0627\u06cc\u06cc (Induced Pluripotent Stem Cell) \u06a9\u06cc\u0641\u06cc\u062a \u0641\u06cc\u0628\u0631\u0648\u0628\u0644\u0627\u0633\u062a \u0647\u0627\u06cc \u062c\u0646\u06cc\u0646\u06cc \u0645\u0648\u0634\u06cc (MEF) \u0647\u0627 \u062a\u0648\u0633\u0637 \u0633\u0648\u06cc\u0647 \u0645\u0648\u0634\u06cc \u0645\u0627\u0646\u0646\u062f CF-1 \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc \u0634\u062f\u0647 \u0627\u0633\u062a. MEF \u0647\u0627 \u0648 \u0645\u0648\u0627\u062f \u0645\u063a\u0630\u06cc \u06a9\u0647 \u0627\u0632 \u0622\u0646\u0647\u0627 \u062a\u0631\u0634\u062d \u0634\u062f\u0647 \u0648 \u0628\u0647 \u0639\u0644\u0627\u0648\u0647 \u0645\u062d\u06cc\u0637 \u0631\u0648\u06cc\u06cc \u06a9\u0647 \u0627\u0632 \u0631\u0648\u06cc \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0641\u06cc\u0628\u0631\u0648\u0628\u0644\u0627\u0633\u062a \u062c\u0645\u0639 \u0622\u0648\u0631\u06cc \u0645\u06cc \u0634\u0648\u062f\u060c \u0628\u0627\u06cc\u062f \u062d\u0627\u0648\u06cc \u063a\u0644\u0638\u062a \u0647\u0627\u06cc \u0645\u0637\u0644\u0648\u0628 Activin A\u060c Gremlin \u0648 Tgf\u03b2\u06f1 \u0645\u0648\u0631\u062f \u0646\u06cc\u0627\u0632 \u0628\u0631\u0627\u06cc \u0645\u0633\u06cc\u0631\u0647\u0627\u06cc Activin \/ Nodal \u0648 FGF \u00a0\u0628\u0627\u0634\u0646\u062f \u062a\u0627 \u0628\u062a\u0648\u0627\u0646\u0646\u062f \u062e\u0648\u062f \u0646\u0648\u0632\u0627\u06cc\u06cc (self-renewal) \u0648 \u067e\u0631\u062a\u0648\u0627\u0646\u06cc \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0628\u0646\u06cc\u0627\u062f\u06cc \u0631\u0627 \u062d\u0641\u0638 \u06a9\u0646\u0646\u062f.<\/p>\n<p>\u0628\u0647 \u0637\u0648\u0631 \u06a9\u0644\u06cc\u060c <strong>\u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0628\u0646\u06cc\u0627\u062f\u06cc \u062c\u0646\u06cc\u0646\u06cc \u0627\u0646\u0633\u0627\u0646\u06cc<\/strong><strong> (hESCs)<\/strong> \u0648 <strong>\u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0628\u0646\u06cc\u0627\u062f\u06cc \u067e\u0631\u062a\u0648\u0627\u0646 \u0627\u0644\u0642\u0627\u06cc\u06cc \u0627\u0646\u0633\u0627\u0646\u06cc (<\/strong><strong>hiPSCs)<\/strong>\u00a0\u0645\u06cc \u062a\u0648\u0627\u0646\u0646\u062f \u062a\u062d\u062a \u0634\u0631\u0627\u06cc\u0637 \u0645\u062a\u063a\u06cc\u0631\u06cc \u06a9\u0634\u062a \u062f\u0627\u062f\u0647 \u0634\u0648\u0646\u062f. \u0628\u0627 \u0627\u06cc\u0646 \u062d\u0627\u0644\u060c \u0627\u06cc\u062c\u0627\u062f \u06cc\u06a9 \u0633\u06cc\u0633\u062a\u0645 \u0645\u0648\u062b\u0631 \u0628\u0631\u0627\u06cc \u06a9\u0634\u062a \u0627\u06cc\u0646 \u0633\u0644\u0648\u0644 \u0647\u0627 \u0622\u0633\u0627\u0646 \u0646\u06cc\u0633\u062a. \u0627\u0632 \u0622\u0646\u062c\u0627 \u06a9\u0647 \u0634\u0631\u0627\u06cc\u0637 \u06a9\u0634\u062a \u0633\u0644\u0648\u0644 \u0645\u06cc \u062a\u0648\u0627\u0646\u062f \u0628\u0631 \u0628\u06cc\u0627\u0646 \u0698\u0646 \u062a\u0627\u062b\u06cc\u0631 \u06af\u0630\u0627\u0631 \u0628\u0627\u0634\u062f\u060c \u062f\u0631\u0633\u0644\u0648\u0644 \u0647\u0627\u06cc hESC \u0647\u0627 \u0648 hiPSC \u0647\u0627 \u060c \u0628\u0647\u06cc\u0646\u0647 \u0633\u0627\u0632\u06cc \u0648 \u0627\u0633\u062a\u0627\u0646\u062f\u0627\u0631\u062f \u0633\u0627\u0632\u06cc \u0631\u0648\u0634 \u06a9\u0634\u062a \u0628\u0633\u06cc\u0627\u0631 \u0645\u0647\u0645 \u0627\u0633\u062a.<\/p>\n<p>\u06a9\u0634\u062a \u0644\u0627\u06cc\u0646 \u0647\u0627\u06cc \u0633\u0644\u0648\u0644\u06cc hESC \u0627\u0628\u062a\u062f\u0627 \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 MEF \u0647\u0627 \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 <strong>\u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u063a\u0630\u0627 \u062f\u0647\u0646\u062f\u0647 (Feeder) <\/strong>\u0648 <strong>\u0633\u0631\u0645 \u062c\u0646\u06cc\u0646 \u06af\u0627\u0648\u06cc<\/strong> <strong>(FBS)<\/strong> \u06a9\u0647 \u062d\u0627\u0648\u06cc \u0645\u062d\u06cc\u0637\u06cc \u0645\u0646\u0627\u0633\u0628 \u0628\u0631\u0627\u06cc \u06a9\u0634\u062a \u062a\u0648\u0635\u06cc\u0641 \u0634\u062f\u0647 \u0627\u0633\u062a\u060c \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u06af\u06cc\u0631\u062f. \u0633\u067e\u0633\u060c FBS \u0628\u0627 \u0633\u0631\u0645 (KSR) \u0648 FGF2 \u062c\u0627\u06cc\u06af\u0632\u06cc\u0646 \u0634\u062f\u0647\u060c \u06a9\u0647 \u0628\u0627\u0639\u062b \u0627\u0641\u0632\u0627\u06cc\u0634 \u062a\u06a9\u062b\u06cc\u0631 hESCs \u0645\u06cc \u0634\u0648\u062f. \u062f\u0631 \u0646\u0647\u0627\u06cc\u062a\u060c \u0633\u06cc\u0633\u062a\u0645 \u0647\u0627\u06cc \u06a9\u0634\u062a \u0628\u062f\u0648\u0646(Feeder) \u06a9\u0647 \u0633\u0644\u0648\u0644 \u0647\u0627 \u0645\u06cc \u062a\u0648\u0627\u0646\u0646\u062f \u0628\u0631 \u0631\u0648\u06cc \u067e\u0644\u06cc\u062a \u06a9\u0634\u062a \u067e\u0648\u0634\u06cc\u062f\u0647 \u0634\u062f\u0647 \u0627\u0632 <strong>\u0645\u0627\u062a\u0631\u06cc\u0698\u0644<\/strong> \u0642\u0631\u0627\u0631 \u0628\u06af\u06cc\u0631\u0646\u062f \u0648 \u0645\u062d\u06cc\u0637 \u00a0\u06a9\u0627\u0646\u062f\u06cc\u0634\u0646 \u0634\u062f\u0647 \u0645\u062d\u062a\u0648\u06cc \u0633\u0631\u0645 (KSR) \u0645\u062d\u06cc\u0637 \u00a0\u06a9\u0627\u0646\u062f\u06cc\u0634\u0646 \u0634\u062f\u0647 \u0628\u0647 \u0648\u0627\u0633\u0637\u0647 (MEF) \u0627\u0646\u062a\u0642\u0627\u0644 \u062f\u0627\u062f\u0647 \u0634\u0648\u0646\u062f. \u067e\u0633 \u0627\u0632 \u0622\u0646\u060c \u0634\u0631\u0627\u06cc\u0637 \u06a9\u0634\u062a hESCs \u0628\u0647 \u0633\u0645\u062a \u0645\u062d\u06cc\u0637 \u0647\u0627\u06cc \u0628\u062f\u0648\u0646 Feeder \u0648 \u0645\u062d\u06cc\u0637 \u0647\u0627\u06cc \u06a9\u0627\u0646\u062f\u06cc\u0634\u0646 \u0634\u062f\u0647 \u0634\u06cc\u0645\u06cc\u0627\u06cc\u06cc \u0628\u0627 \u063a\u0644\u0638\u062a \u0645\u0634\u062e\u0635 \u067e\u06cc\u0634 \u0628\u0631\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f. \u0646\u06a9\u062a\u0647 \u0645\u0647\u0645 \u0627\u06cc\u0646 \u0627\u0633\u062a \u06a9\u0647 \u0634\u0648\u0627\u0647\u062f\u06cc \u0645\u0628\u0646\u06cc \u0628\u0631 \u062a\u0641\u0627\u0648\u062a \u062f\u0631 \u0641\u0627\u06a9\u062a\u0648\u0631 \u0647\u0627\u06cc \u0631\u0634\u062f \u062a\u0648\u0644\u06cc\u062f \u0634\u062f\u0647 \u062f\u0631 \u0633\u0644\u0648\u0644\u0647\u0627\u06cc feeder \u0627\u0646\u0633\u0627\u0646\u06cc \u0648 \u0645\u0648\u0634\u06cc \u0648\u062c\u0648\u062f \u062f\u0627\u0631\u062f. \u062a\u062c\u0632\u06cc\u0647 \u0648 \u062a\u062d\u0644\u06cc\u0644 \u0627\u0632 transcriptomes \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc Feeder \u0645\u0648\u0634\u06cc \u0648 \u0627\u0646\u0633\u0627\u0646\u06cc \u0646\u0634\u0627\u0646 \u062f\u0627\u062f \u06a9\u0647 \u062a\u0641\u0627\u0648\u062a \u0647\u0627\u06cc \u0642\u0627\u0628\u0644 \u062a\u0648\u062c\u0647\u06cc \u0628\u06cc\u0646 \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u062d\u0645\u0627\u06cc\u062a\u06cc \u0648 \u063a\u06cc\u0631 \u062d\u0627\u0645\u06cc \u0648\u062c\u0648\u062f \u062f\u0627\u0631\u062f. Exogenous FGF2 \u0628\u0631\u0627\u06cc \u062d\u0641\u0638 \u062e\u0648\u062f \u0646\u0648\u0632\u0627\u06cc\u06cc hESCs \u0648 hiPSCs \u0628\u0633\u06cc\u0627\u0631 \u0645\u0647\u0645 \u0627\u0633\u062a \u0648 \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u0639\u0627\u0645\u0644 \u06a9\u0644\u06cc\u062f\u06cc \u062a\u0646\u0638\u06cc\u0645 \u0628\u06cc\u0627\u0646 Tgf\u03b2\u06f1\u060c Activin A \u0648 Gremlin \u0622\u0646\u062a\u0627\u06af\u0648\u0646\u06cc\u0633\u062a(BMP) \u062f\u0631 \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0641\u06cc\u062f\u0631 \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc \u0634\u062f\u0647 \u0627\u0633\u062a. \u0646\u0634\u0627\u0646 \u062f\u0627\u062f\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 <strong>Activin A<\/strong> \u0628\u06cc\u0627\u0646 OCT4<strong>\u060c <\/strong><strong>\u00a0<\/strong>SOX2 \u0648 NANOG\u00a0\u0631\u0627 \u062f\u0631 hESCs15-16 \u0627\u0644\u0642\u0627 \u0645\u06cc \u06a9\u0646\u062f.<\/p>\n<p>\u0628\u0631\u0627\u06cc \u06a9\u0634\u062a \u0637\u0648\u0644\u0627\u0646\u06cc \u0645\u062f\u062a\u060chESC \u0647\u0627 \u0648 hiPSC \u0647\u0627 \u0645\u06cc \u062a\u0648\u0627\u0646\u0646\u062f \u0628\u0631 \u0631\u0648\u06cc MEF \u0647\u0627\u06cc\u06cc \u06a9\u0647 \u062a\u06a9\u062b\u06cc\u0631\u0634\u0627\u0646 \u0645\u062a\u0648\u0642\u0641 \u0634\u062f\u0647 \u06cc\u0627 \u062a\u062d\u062a \u0634\u0631\u0627\u06cc\u0637 \u0628\u062f\u0648\u0646 \u0641\u06cc\u062f\u0631(Feeder-free conditions)\u062f\u0631 MEF-CM(MEF-Conditioned Medium) \u0628\u0631 \u0631\u0648\u06cc \u067e\u0644\u06cc\u062a \u0647\u0627\u06cc \u067e\u0648\u0634\u06cc\u062f\u0647 \u0634\u062f\u0647 \u0628\u0627 Matrigel \u0631\u0634\u062f \u06a9\u0646\u0646\u062f \u062a\u0627 \u062d\u0627\u0644\u062a \u0628\u062f\u0648\u0646 \u062a\u0645\u0627\u06cc\u0632 \u0622\u0646\u0647\u0627 \u062d\u0641\u0638 \u0634\u0648\u062f \u0648 \u0628\u0647 \u0627\u0635\u0637\u0644\u0627\u062d Stemness \u0622\u0646\u0647\u0627 \u062a\u0627 \u062d\u062f \u0632\u06cc\u0627\u062f\u06cc \u0646\u06af\u0647 \u062f\u0627\u0634\u062a\u0647 \u0634\u0648\u062f. \u0645\u0648\u0641\u0642\u06cc\u062a \u0647\u0631 \u062f\u0648 \u0634\u0631\u0627\u06cc\u0637 \u06a9\u0634\u062a \u0628\u0647 \u0637\u0648\u0631 \u06a9\u0627\u0645\u0644 \u0628\u0633\u062a\u06af\u06cc \u0628\u0647 \u06a9\u06cc\u0641\u06cc\u062a \u0633\u0644\u0648\u0644\u0647\u0627\u06cc \u0641\u06cc\u062f\u0631 \u062f\u0627\u0631\u062f\u060c \u0632\u06cc\u0631\u0627 \u0622\u0646\u0647\u0627 \u0628\u0647 \u0637\u0648\u0631 \u0645\u0633\u062a\u0642\u06cc\u0645 \u0628\u0631 \u0631\u0634\u062f hESC \u0647\u0627 \u062a\u0627\u062b\u06cc\u0631 \u0645\u06cc \u06af\u0630\u0627\u0631\u0646\u062f.<\/p>\n<p>\u062a\u0635\u0648\u06cc\u0631 \u0628\u0627\u0644\u0627\u06cc \u0635\u0641\u062d\u0647 \u0645\u0648\u0631\u0641\u0648\u0644\u0648\u0698\u06cc \u062a\u06cc\u067e\u06cc\u06a9 \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc hESCs \u0648 hiPSCs \u06a9\u0634\u062a \u062f\u0627\u062f\u0647 \u0634\u062f\u0647 \u0631\u0627 \u0646\u0634\u0627\u0646 \u0645\u06cc \u062f\u0647\u062f.<\/p>\n<p>&nbsp;<\/p>\n<h3><strong>\u067e\u0631\u0648\u062a\u06a9\u0644\/ \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0631\u0648\u0634 \u0628\u0647\u06cc\u0646\u0647 \u062c\u0647\u062a \u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u0648 \u06a9\u0634\u062a \u0641\u06cc\u0628\u0631\u0648\u0628\u0644\u0627\u0633\u062a \u0647\u0627\u06cc \u062c\u0646\u06cc\u0646\u06cc \u0645\u0648<\/strong><strong>\u0634\u06cc<\/strong><\/h3>\n<ul>\n<li>\u0627\u0628\u062a\u062f\u0627 \u06cc\u06a9 \u0645\u0648\u0634 \u0628\u0627\u0631\u062f\u0627\u0631 \u0631\u0627 \u0628\u0627 \u0631\u0648\u0634 \u0647\u0627\u06cc\u06cc \u06a9\u0647 \u062f\u0631 \u0647\u0631 \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647 \u0627\u0633\u062a\u0627\u0646\u062f\u0627\u0631\u062f \u0634\u062f\u0647 \u0645\u06cc \u06a9\u0634\u06cc\u0645.<\/li>\n<li>\u0627\u0646\u0633\u062f\u0627\u062f \u062f\u0647\u0627\u0646\u0647 \u06cc \u0631\u062d\u0645\u06cc \u0648 \u0628\u0627 \u0627\u062a\u0627\u0646\u0648\u0644 \u06f7\u06f0% \u0648 \u0628\u0627 PBS+ \u0634\u0633\u062a\u0634\u0648 \u062f\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f.<\/li>\n<li>\u062a\u0645\u0627\u0645\u06cc \u0645\u0631\u0627\u062d\u0644 \u0628\u0639\u062f\u06cc \u062f\u0631 \u0632\u06cc\u0631 \u0647\u0648\u062f \u0648 \u062a\u062d\u062a \u0634\u0631\u0627\u06cc\u0637 \u0622\u0633\u067e\u062a\u06cc\u06a9 \u0648 \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u062a\u062c\u0647\u06cc\u0632\u0627\u062a \u0627\u0633\u062a\u0631\u06cc\u0644 \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u06af\u06cc\u0631\u062f.<\/li>\n<li>\u0634\u0627\u062e \u0647\u0627 \u06cc \u0631\u062d\u0645\u06cc \u0628\u0647 \u062f\u0627\u062e\u0644 \u06cc\u06a9 \u067e\u0644\u06cc\u062a \u06a9\u0634\u062a \u0627\u0646\u062a\u0642\u0627\u0644 \u062f\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f \u0648 \u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u0647\u0631 \u062c\u0646\u06cc\u0646 \u0627\u0632 \u062c\u0641\u062a \u062e\u0648\u062f \u0648 \u06a9\u06cc\u0633\u0647 \u062c\u0646\u06cc\u0646\u06cc \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u06af\u06cc\u0631\u062f.<\/li>\n<li>\u0627\u0646\u062f\u0627\u0645 \u0633\u0631 \u0648 \u0627\u0646\u062f\u0627\u0645 \u0647\u0627\u06cc \u0642\u0631\u0645\u0632 \u0631\u0627 \u0628\u0634\u06a9\u0646\u06cc\u062f \u0648 \u062f\u0631 PBS \u0628\u0634\u0648\u06cc\u06cc\u062f \u0648 \u0647\u0645\u0647 \u062c\u0646\u06cc\u0646 \u0647\u0627 \u0631\u0627 \u062f\u0631 \u06cc\u06a9 \u067e\u0644\u06cc\u062a \u06a9\u0634\u062a \u062a\u0645\u06cc\u0632 \u0642\u0631\u0627\u0631 \u062f\u0647\u06cc\u062f. \u062f\u0631 \u0646\u0647\u0627\u06cc\u062a \u0628\u0627\u0641\u062a \u0647\u0627 \u0631\u0627 \u0628\u0627 \u06cc\u06a9 \u062a\u06cc\u063a \u0627\u0633\u062a\u0631\u06cc\u0644 \u062e\u0631\u062f \u06a9\u0646\u06cc\u062f \u062a\u0627 \u0627\u0645\u06a9\u0627\u0646 \u067e\u06cc\u067e\u062a \u06a9\u0631\u062f\u0646 \u0641\u0631\u0627\u0647\u0645 \u0634\u0648\u062f.<\/li>\n<li>\u06f1 \u0645\u06cc\u0644\u06cc \u0644\u06cc\u062a\u0631 \u0627\u0632 \u062a\u0631\u06cc\u067e\u0633\u06cc\u0646 \u06f0.\u06f0\u06f5% \u062f\u0631 \u0647\u0631 \u062c\u0646\u06cc\u0646 \u0627\u0636\u0627\u0641\u0647 \u0645\u06cc \u06a9\u0646\u06cc\u0645.<\/li>\n<li>\u0628\u0627\u0641\u062a \u0631\u0627 \u0628\u0647 \u06cc\u06a9 \u0641\u0627\u0644\u06a9\u0648\u0646 \u06f5\u06f0 \u0627\u0646\u062a\u0642\u0627\u0644 \u062f\u0647\u06cc\u062f \u0648 \u0628\u0647 \u0645\u062f\u062a \u06f1\u06f5 \u062f\u0642\u06cc\u0642\u0647 \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f3\u06f7 \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u06af\u0631\u0627\u062f \u0627\u0646\u06a9\u0648\u0628\u0647 \u06a9\u0646\u06cc\u062f\u060c \u067e\u0633 \u0627\u0632 \u0647\u0631 \u06f5 \u062f\u0642\u06cc\u0642\u0647 \u0627\u0646\u06a9\u0648\u0628\u0627\u0633\u06cc\u0648\u0646\u060c \u0633\u0644\u0648\u0644 \u0647\u0627 \u0631\u0627 \u0628\u0647 \u0648\u0633\u06cc\u0644\u0647 \u067e\u06cc\u067e\u062a \u06a9\u0631\u062f\u0646 \u0628\u0627\u0644\u0627 \u0648 \u067e\u0627\u06cc\u06cc\u0646 \u0628\u0628\u0631\u06cc\u062f \u062a\u0627 \u06a9\u0627\u0645\u0644\u0627 \u0627\u0632 \u0647\u0645 \u062c\u062f\u0627 \u0634\u0648\u0646\u062f.<\/li>\n<li>\u0622\u0646\u0632\u06cc\u0645 \u0631\u0627 \u0628\u0627 \u0627\u0636\u0627\u0641\u0647 \u06a9\u0631\u062f\u0646 \u06f1 \u0645\u06cc\u0644\u06cc \u0644\u06cc\u062a\u0631 \u0627\u0632 \u0645\u062d\u06cc\u0637 MEF \u0622\u0645\u0627\u062f\u0647 \u0634\u062f\u0647 \u062e\u0646\u062b\u06cc \u06a9\u0646\u06cc\u062f. \u0645\u062d\u06cc\u0637 \u06a9\u0634\u062a MEF(\u0647\u0645\u0647 \u0645\u0648\u0627\u062f \u0628\u0627 \u0647\u0645 \u062a\u0631\u06a9\u06cc\u0628 \u0634\u062f\u0647 \u0648 \u0633\u067e\u0633 \u0641\u06cc\u0644\u062a\u0631 \u0645\u06cc \u0634\u0648\u0646\u062f\u061b \u06f4\u06f5\u06f0ml DMEM, 50ml FBS10%, 5ml L-glutamin, 5mlPenecillin-Streptomycin, )<\/li>\n<li>\u0633\u067e\u0633 \u0633\u0644\u0648\u0644 \u0647\u0627 \u0628\u0647 \u0622\u0631\u0627\u0645\u06cc \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0634\u062f\u0647 \u0628\u0627 \u0633\u0631\u0639\u062a \u06a9\u0645(\u06f3\u06f0\u06f0g) \u0628\u0647 \u0645\u062f\u062a \u06f5 \u062f\u0642\u06cc\u0642\u0647 \u0648 \u0645\u062d\u06cc\u0637 \u0631\u0648\u06cc\u06cc \u0628\u0647 \u0622\u0631\u0627\u0645\u06cc \u0628\u0631\u062f\u0627\u0634\u062a\u0647 \u0645\u06cc \u0634\u0648\u062f \u0648 \u067e\u0644\u062a \u0633\u0644\u0648\u0644\u06cc \u062f\u0648\u0628\u0627\u0631\u0647 \u062f\u0631\u0645\u062d\u06cc\u0637 MEF \u06af\u0631\u0645 \u062d\u0644 \u0645\u06cc \u0634\u0648\u062f.<\/li>\n<li>\u0628\u0647 \u0637\u0648\u0631 \u0645\u0639\u0645\u0648\u0644 \u062f\u0631 \u0647\u0631 \u0641\u0644\u0627\u0633\u06a9 T150 \u0645\u062d\u062a\u0648\u06cc \u06f3 \u062a\u0627\u06f4 \u062c\u0646\u06cc\u0646 \u0645\u06cc \u0628\u0627\u0634\u062f \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u0631\u0648\u0634 Preplate \u06a9\u0631\u062f\u0646 (\u0628\u0627\u0639\u062b \u0634\u062f\u0647 \u0641\u06cc\u0628\u0631\u0648\u0628\u0644\u0627\u0633\u062a \u0647\u0627 \u06a9\u0647 \u062a\u0648\u0627\u0646\u0627\u06cc\u06cc \u0627\u062a\u0635\u0627\u0644 \u0628\u0647 \u067e\u0644\u06cc\u062a \u0647\u0627\u06cc \u067e\u0648\u0634\u06cc\u062f\u0647 \u0634\u062f\u0647 \u0628\u0627 \u0698\u0644\u0627\u062a\u06cc\u0646 \u0631\u0627 \u062f\u0627\u0631\u0646\u062f) \u0628\u0647 \u062e\u0648\u0628\u06cc \u0628\u0686\u0633\u0628\u0646\u062f.<\/li>\n<li>\u067e\u0633 \u0627\u0632 \u06af\u0630\u0634\u062a \u06f2\u06f4 \u0633\u0627\u0639\u062a \u06a9\u0647 \u062a\u0631\u0627\u06a9\u0645 \u0633\u0644\u0648\u0644\u06cc \u0628\u0647 \u06f8\u06f0-\u06f9\u06f0% \u0631\u0633\u06cc\u062f \u06a9\u0647 \u062f\u0631 \u0627\u06cc\u0646 \u0645\u0631\u062d\u0644\u0647 \u0633\u0644\u0648\u0644 \u0647\u0627 \u067e\u0627\u0633\u0627\u0698\u06f0 \u0645\u062d\u0633\u0648\u0628 \u0645\u06cc \u0634\u0648\u0646\u062f \u0648 \u0628\u0631\u0627\u06cc \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0647\u0627\u06cc \u0628\u0639\u062f\u06cc \u0641\u0631\u06cc\u0632 \u0645\u06cc \u0634\u0648\u0646\u062f.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-4352 aligncenter\" src=\"http:\/\/darwino.ir\/wp-content\/uploads\/2019\/07\/IMG_20190706_082004_448-98x300.jpg\" alt=\"\" width=\"311\" height=\"953\" srcset=\"http:\/\/darwino.ir\/blogs\/wp-content\/uploads\/2019\/07\/IMG_20190706_082004_448-98x300.jpg 98w, http:\/\/darwino.ir\/blogs\/wp-content\/uploads\/2019\/07\/IMG_20190706_082004_448.jpg 196w\" sizes=\"auto, (max-width: 311px) 100vw, 311px\" \/><\/p>\n<p style=\"text-align: center;\">\u00a0 <strong>\u0645\u0631\u0627\u062d\u0644 \u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u0641\u06cc\u0628\u0631\u0648\u0628\u0644\u0627\u0633\u062a (MEF) <\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u0648 \u0627\u0633\u062a\u062e\u0631\u0627\u062c feeder \u0647\u0627 \u0628\u0631\u0627\u06cc \u06a9\u0634\u062a \u0633\u0644\u0648\u0644 \u0647\u0627 \u0648 \u0641\u0631\u0627\u0647\u0645 \u0622\u0648\u0631\u06cc \u0645\u062d\u06cc\u0637 \u062a\u063a\u0630\u06cc\u0647 \u0627\u06cc \u0645\u0646\u0627\u0633\u0628 \u0628\u0631\u0627\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0622\u0646\u0647\u0627 \u0628\u0633\u06cc\u0627\u0631 \u0645\u0647\u0645 \u0627\u0633\u062a \u0639\u0644\u0627\u0648\u0647 \u0628\u0631 \u0622\u0646 \u0645\u0631\u0627\u062d\u0644 \u0627\u0646\u062c\u0627\u0645 \u0627\u06cc\u0646 \u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u0627\u0632 \u062d\u06cc\u0648\u0627\u0646 \u0628\u0627\u06cc\u062f \u0628\u0627 \u062f\u0642\u062a \u0628\u0633\u06cc\u0627\u0631\u06cc \u0627\u0646\u062c\u0627\u0645 \u06af\u06cc\u0631\u062f.<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-4350 aligncenter\" src=\"http:\/\/darwino.ir\/wp-content\/uploads\/2019\/07\/IMG_20190706_053114_801-300x131.jpg\" alt=\"\" width=\"417\" height=\"182\" srcset=\"http:\/\/darwino.ir\/blogs\/wp-content\/uploads\/2019\/07\/IMG_20190706_053114_801-200x87.jpg 200w, http:\/\/darwino.ir\/blogs\/wp-content\/uploads\/2019\/07\/IMG_20190706_053114_801-300x131.jpg 300w, http:\/\/darwino.ir\/blogs\/wp-content\/uploads\/2019\/07\/IMG_20190706_053114_801.jpg 400w\" sizes=\"auto, (max-width: 417px) 100vw, 417px\" \/><\/p>\n<p style=\"text-align: center;\">\u00a0<strong>\u0645\u0648\u0631\u0641\u0648\u0644\u0648\u0698\u06cc \u0645\u0639\u0645\u0648\u0644 mouse embryonic fibroblasts (MEFs)\u00a0<\/strong><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u270d\u00a0\u06a9\u0627\u0631\u0628\u0631\u062f \u062a\u06a9\u0646\u06cc\u06a9 \u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u0648 \u06a9\u0634\u062a \u0641\u06cc\u0628\u0631\u0648\u0628\u0644\u0627\u0633\u062a \u062c\u0646\u06cc\u0646\u06cc \u0645\u0648\u0634 \u062f\u0631 \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647 [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":4354,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[30],"tags":[1508,1509,1504,1499,684,1503,1505,1501,619,1507,1500,1506,1299,1502,617,618],"blog-category":[3077,3078,3086,3073],"darwinoplus-category":[],"class_list":{"0":"post-4342","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","6":"hentry","7":"category-blog","8":"tag-hescs","9":"tag-_mef","10":"tag-______","11":"tag-_____","12":"tag-___","15":"tag-_______","16":"tag-____-__","18":"tag-____","19":"tag-_pdf______","20":"tag-________","22":"tag-__","24":"blog-category-biotechnology","25":"blog-category-histology","26":"blog-category-microbial","27":"blog-category-microbiology"},"_links":{"self":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/4342","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/comments?post=4342"}],"version-history":[{"count":25,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/4342\/revisions"}],"predecessor-version":[{"id":23288,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/4342\/revisions\/23288"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media\/4354"}],"wp:attachment":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media?parent=4342"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/categories?post=4342"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/tags?post=4342"},{"taxonomy":"blog-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/blog-category?post=4342"},{"taxonomy":"darwinoplus-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/darwinoplus-category?post=4342"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}