{"id":4117,"date":"2019-07-03T03:56:50","date_gmt":"2019-07-03T03:56:50","guid":{"rendered":"http:\/\/darwino.ir\/blogs\/?p=4117"},"modified":"2020-03-27T22:18:35","modified_gmt":"2020-03-27T18:48:35","slug":"real-time-pcr-section-2","status":"publish","type":"post","link":"http:\/\/darwino.ir\/blogs\/real-time-pcr-section-2\/","title":{"rendered":"\u062a\u06a9\u0646\u06cc\u06a9 Real-Time PCR &#8211; \u0628\u062e\u0634 \u062f\u0648\u0645 (\u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a)"},"content":{"rendered":"<h2><strong>\u270d <\/strong><strong>\u0633\u06cc\u06a9\u0644 \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u062f\u0631 \u0631\u0648\u0634 \u0633\u0627\u06cc\u0628\u0631\u06af\u0631\u06cc\u0646<\/strong><\/h2>\n<p>RealTime PCR \u0646\u0648\u0639\u06cc PCR \u0645\u06cc \u0628\u0627\u0634\u062f \u06a9\u0647 \u0628\u0647 \u062a\u0648\u0627\u0644\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0634\u062f\u0647 \u0628\u0647 \u0635\u0648\u0631\u062a \u06a9\u0645\u06cc \u0633\u0646\u062c\u06cc\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f. \u0628\u0631\u0627\u06cc \u0627\u06cc\u0646 \u06a9\u0627\u0631\u00a0 \u0627\u0632 \u062f\u0633\u062a\u06af\u0627\u0647 real-time PCR (\u0631\u06cc\u0644 \u062a\u0627\u06cc\u0645 \u067e\u06cc \u0633\u06cc \u0622\u0631) \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f \u06a9\u0647 \u0646\u0648\u0639\u06cc \u062a\u0631\u0645\u0627\u0644 \u0633\u0627\u06cc\u06a9\u0644\u0631 \u0645\u06cc \u0628\u0627\u0634\u062f \u06a9\u0647 \u0627\u0632 \u06cc\u06a9 \u0633\u06cc\u0633\u062a\u0645 \u0627\u067e\u062a\u06cc\u06a9\u06cc \u0628\u0647\u0631\u0647 \u0628\u0631\u062f\u0647 \u0648 \u0645\u06cc\u0632\u0627\u0646 DNA \u0647\u0631 \u0646\u0645\u0648\u0646\u0647 \u062a\u0648\u0633\u0637 \u0622\u0646 \u0645\u062d\u0627\u0633\u0628\u0647 \u0645\u06cc \u06af\u0631\u062f\u062f.<\/p>\n<p>\u0632\u0645\u0627\u0646\u06cc \u06a9\u0647 <a href=\"http:\/\/darwino.ir\/rt-pcr-technique-for-synthesizng-dna\/\"><strong>\u0633\u0646\u062a\u0632 cDNA<\/strong><\/a> \u0627\u0646\u062c\u0627\u0645 \u0634\u062f\u060c \u067e\u0633 \u0627\u0632 \u0622\u0645\u0627\u062f\u0647 \u0633\u0627\u0632\u06cc \u0646\u0645\u0648\u0646\u0647 \u0628\u0647 \u0645\u0646\u0638\u0648\u0631 \u0648\u0631\u0648\u062f \u0628\u0647 \u0633\u06cc\u06a9\u0644 \u0647\u0627\u06cc \u067e\u0644\u06cc\u0645\u0631\u06cc\u0632\u0647 \u0634\u062f\u0646\u060c \u0628\u0647 \u0646\u0645\u0648\u0646\u0647 DNA \u0631\u0646\u06af\u06cc \u062a\u062d\u062a \u0639\u0646\u0648\u0627\u0646 \u0633\u0627\u06cc\u0628\u0631 \u06af\u0631\u06cc\u0646 (SYBR Green) \u06a9\u0647 \u0628\u0647 DNA \u0645\u062a\u0635\u0644 \u0645\u06cc \u06af\u0631\u062f\u062f\u060c \u0627\u0636\u0627\u0641\u0647 \u0645\u06cc \u06af\u0631\u062f\u062f \u0648 \u06cc\u0627 \u0627\u0632 \u067e\u0631\u0648\u0628 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f. \u062f\u0633\u062a\u06af\u0627\u0647 real-time PCR \u0628\u0627 \u062e\u0627\u0635\u06cc\u062a \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u062a\u06cc \u0633\u0627\u06cc\u0628\u0631 \u06af\u0631\u06cc\u0646 (SYBR Green)\u00a0 \u0648 \u06cc\u0627 \u067e\u0631\u0648\u0628 \u0628\u0631\u0627\u06cc \u0647\u0631 \u0646\u0645\u0648\u0646\u0647 \u06cc\u06a9 \u06af\u0631\u0627\u0641 \u062a\u06a9\u062b\u06cc\u0631 \u062a\u0631\u0633\u06cc\u0645 \u0645\u06cc \u06a9\u0646\u062f \u06a9\u0647 \u0647\u0631 \u0646\u0642\u0637\u0647 \u0627\u0632 \u06af\u0631\u0627\u0641 \u062a\u06a9\u062b\u06cc\u0631 \u0646\u0634\u0627\u0646 \u062f\u0647\u0646\u062f\u0647 \u0645\u06cc\u0632\u0627\u0646 \u0627\u062a\u0635\u0627\u0644 \u0633\u0627\u06cc\u0628\u0631 \u06af\u0631\u06cc\u0646 \u0648 \u06cc\u0627 \u067e\u0631\u0648\u0628 \u0628\u0647 \u0631\u0634\u062a\u0647 DNA \u0645\u06cc \u0628\u0627\u0634\u062f \u0648 \u062f\u0631 \u0646\u0647\u0627\u06cc\u062a \u06af\u0631\u0627\u0641 \u06a9\u0647 \u062f\u0631 \u0646\u0645\u0627\u06cc\u0634\u06af\u0631 \u0645\u062a\u0635\u0644 \u0634\u062f\u0647 \u0628\u0647 real-time PCR\u00a0 \u0646\u0634\u0627\u0646 \u062f\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f \u060c \u0628\u0647 \u062d\u062f \u0622\u0633\u062a\u0627\u0646\u0647 \u062e\u0648\u062f \u0645\u06cc \u0631\u0633\u062f \u0648 \u0627\u06cc\u0646 \u0645\u06cc\u0632\u0627\u0646 \u0646\u0634\u0627\u0646 \u062f\u0647\u0646\u062f\u0647 \u0645\u06cc\u0632\u0627\u0646 DNA \u0628\u06cc\u0627\u0646 \u0634\u062f\u0647 \u062f\u0631 \u0647\u0631 \u0646\u0645\u0648\u0646\u0647 \u0645\u06cc \u0628\u0627\u0634\u062f.<\/p>\n<p>\u062a\u06a9\u062b\u06cc\u0631 \u062a\u0648\u0627\u0644\u06cc DNA \u0647\u062f\u0641 \u0628\u0647 \u0635\u0648\u0631\u062a \u0646\u0645\u0627\u06cc\u06cc \u0645\u06cc \u0628\u0627\u0634\u062f \u0628\u0647 \u0627\u06cc\u0646 \u0635\u0648\u0631\u062a \u06a9\u0647 \u06cc\u06a9 \u0627\u0644\u06af\u0648 \u0628\u0647 \u062f\u0648\u060c \u062f\u0648 \u0627\u0644\u06af\u0648 \u0628\u0647 \u0686\u0647\u0627\u0631 \u0648 \u0686\u0647\u0627\u0631 \u0627\u0644\u06af\u0648 \u0628\u0647 \u0647\u0634\u062a \u0627\u0644\u06af\u0648 \u0648 &#8230;. \u062a\u0628\u062f\u06cc\u0644 \u0645\u06cc\u200c\u0634\u0648\u062f \u0648 \u0627\u06cc\u0646 \u0631\u0648\u0646\u062f \u0627\u062f\u0627\u0645\u0647 \u067e\u06cc\u062f\u0627 \u0645\u06cc\u200c\u06a9\u0646\u062f. \u062f\u0631 \u0627\u0628\u062a\u062f\u0627\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0627\u06cc\u0646 \u0645\u0648\u0636\u0648\u0639 \u062f\u0631\u0633\u062a \u0628\u0647 \u0646\u0638\u0631 \u0645\u06cc\u200c\u0631\u0633\u062f. \u0627\u0645\u0627\u060c \u062a\u06a9\u062b\u06cc\u0631 \u0646\u0645\u0627\u06cc\u06cc \u062a\u0627 \u0627\u0628\u062f \u0627\u062f\u0627\u0645\u0647 \u0646\u0645\u06cc \u06cc\u0627\u0628\u062f \u0648 \u0628\u0647 \u0637\u0648\u0631 \u0645\u0639\u0645\u0648\u0644 \u062a\u0627 \u0686\u0631\u062e\u0647 \u06f3\u06f5 \u0633\u0631\u0639\u062a \u0648\u0627\u06a9\u0646\u0634 \u06a9\u0627\u0647\u0634 \u067e\u06cc\u062f\u0627 \u0645\u06cc \u06a9\u0646\u062f. \u0632\u06cc\u0631\u0627\u060c \u0645\u0642\u062f\u0627\u0631 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627 \u0648 dNTP\u0647\u0627 \u06a9\u0645 \u0645\u06cc \u0634\u0648\u062f \u0648 \u0628\u0627\u0632\u062f\u0647 DNA \u067e\u0644\u06cc\u0645\u0631\u0627\u0632 \u06a9\u0627\u0633\u062a\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a\u061b \u062f\u0646\u0627\u062a\u0648\u0631\u0627\u0633\u06cc\u0648\u0646 DNA \u0647\u0627 \u0628\u0647 \u0637\u0648\u0631 \u06a9\u0627\u0645\u0644 \u0627\u0646\u062c\u0627\u0645 \u0646\u0645\u06cc\u200c \u0634\u0648\u062f\u061b \u0648 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u0648\u0627\u06a9\u0646\u0634 \u062a\u0648\u0633\u0637 \u062e\u0627\u0635\u06cc\u062a \u0646\u0648\u06a9\u0644\u0626\u0627\u0632\u06cc \u067e\u0644\u06cc\u0645\u0631\u0627\u0632 \u0627\u0632 \u0628\u06cc\u0646 \u0645\u06cc\u200c\u0631\u0648\u0646\u062f\u060c \u062f\u0631 \u0646\u062a\u06cc\u062c\u0647 \u0648\u0627\u06a9\u0646\u0634 \u0648\u0627\u0631\u062f \u06cc\u06a9 \u0641\u0627\u0632 \u062e\u0637\u06cc \u0645\u06cc\u200c\u0634\u0648\u062f. \u062f\u0631 \u0627\u06cc\u0646 \u062d\u0627\u0644\u062a \u0645\u0627\u0646\u0646\u062f \u0641\u0627\u0632 \u0644\u06af\u0627\u0631\u06cc\u062a\u0645\u06cc \u062a\u0648\u0627\u0644\u06cc\u200c\u0647\u0627\u06cc \u0627\u0644\u06af\u0648 \u0628\u0647 \u0637\u0648\u0631 \u06a9\u0627\u0645\u0644 \u062f\u0648 \u0628\u0631\u0627\u0628\u0631 \u0646\u0645\u06cc\u200c\u0634\u0648\u0646\u062f \u0648 \u0628\u0627\u0632\u062f\u0647 \u062f\u06cc\u06af\u0631 \u06f1\u06f0\u06f0% \u0646\u06cc\u0633\u062a. \u062a\u0627 \u0627\u06cc\u0646\u06a9\u0647 \u062f\u0631\u0646\u0647\u0627\u06cc\u062a \u062a\u0627 \u0686\u0631\u062e\u0647 \u06f4\u06f0\u0627\u0645\u060c \u0648\u0627\u06a9\u0646\u0634 \u0648\u0627\u0631\u062f \u0645\u0631\u062d\u0644\u0647 \u06a9\u0641\u0647 \u0645\u06cc\u200c\u0634\u0648\u062f \u06a9\u0647 \u062a\u0648\u0642\u0641 \u062a\u06a9\u062b\u06cc\u0631 \u0627\u062a\u0641\u0627\u0642 \u0645\u06cc\u200c\u0627\u0641\u062a\u062f.<\/p>\n<h3><strong>\u0627\u06af\u0631 \u0645\u0646\u062d\u0646\u06cc \u0645\u06cc\u0632\u0627\u0646 \u062a\u06a9\u062b\u06cc\u0631 \u0645\u062d\u0635\u0648\u0644 \u062f\u0631 Real time PCR \u0631\u0627 \u0628\u0631 \u062d\u0633\u0628 \u0633\u06cc\u06a9\u0644 \u0647\u0627 \u062a\u0631\u0633\u06cc\u0645 \u06a9\u0646\u06cc\u0645\u00a0 \u06cc\u06a9 \u0645\u0646\u062d\u0646\u06cc\u00a0 \u06a9\u0647 \u0634\u0627\u0645\u0644 \u06f4 \u0641\u0627\u0632 \u0645\u06cc \u0628\u0627\u0634\u062f \u0627\u06cc\u062c\u0627\u062f \u0645\u06cc \u0634\u0648\u062f \u06a9\u0647 \u0639\u0628\u0627\u0631\u062a \u0627\u0633\u062a \u0627\u0632:<\/strong><\/h3>\n<p><strong>\u0641\u0627\u0632 \u0627\u0648\u0644\/ \u0641\u0627\u0632 <\/strong><strong>Baseline region<\/strong><strong> \/ <\/strong><strong>Linear ground phase<\/strong><strong> :<\/strong> \u062f\u0631 \u0627\u06cc\u0646 \u0641\u0627\u0632 \u0645\u06cc\u0632\u0627\u0646 \u062a\u06a9\u062b\u06cc\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u0628\u0647 \u0645\u06cc\u0632\u0627\u0646\u06cc \u0646\u06cc\u0633\u062a \u06a9\u0647 \u0628\u0627\u0639\u062b \u0627\u0641\u0632\u0627\u06cc\u0634 \u0646\u0648\u0631 \u0641\u0644\u0648\u0631\u0633\u0646\u062a \u0634\u0648\u062f. \u0646\u0648\u0631 \u0641\u0644\u0648\u0631\u0633\u0646\u062a \u0645\u0634\u0627\u0647\u062f\u0647 \u0634\u062f\u0647 \u062f\u0631 \u0627\u06cc\u0646 \u0641\u0627\u0632 \u0627\u0632 cDNA\u060c \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627 \u0648 \u0631\u0646\u06af \u0647\u0627\u06cc \u0641\u0644\u0648\u0631\u0633\u0646\u062a \u0622\u0632\u0627\u062f \u062f\u0631 \u0645\u062d\u06cc\u0637 \u0648\u0627\u06a9\u0646\u0634 \u0645\u0646\u062a\u0634\u0631 \u0645\u06cc \u0634\u0648\u062f \u0628\u0647 \u0647\u0645\u06cc\u0646 \u062f\u0644\u06cc\u0644 \u062f\u0631 \u0627\u06cc\u0646 \u0641\u0627\u0632 \u0645\u06cc\u0632\u0627\u0646 \u0646\u0648\u0631 \u0633\u0627\u0637\u0639 \u0634\u062f\u0647 \u0642\u0627\u0628\u0644 \u0631\u062f\u06cc\u0627\u0628\u06cc \u0646\u06cc\u0633\u062a \u0648 \u0628\u0647 \u0635\u0648\u0631\u062a \u062e\u0637\u06cc \u0645\u06cc \u0628\u0627\u0634\u062f .<\/p>\n<p><strong>\u0641\u0627\u0632 \u062f\u0648\u0645 \/ <\/strong><strong>Exponential phase<\/strong><strong> : <\/strong>\u062f\u0631 \u0627\u06cc\u0646 \u0641\u0627\u0632 \u0645\u06cc\u0632\u0627\u0646 \u0645\u062d\u0635\u0648\u0644 \u062f\u0648 \u0631\u0634\u062a\u0647 \u0627\u06cc \u062f\u0631 \u0647\u0631 \u0686\u0631\u062e\u0647 \u062f\u0648 \u0628\u0631\u0627\u0628\u0631 \u0645\u06cc \u0634\u0648\u062f \u0628\u0646\u0627\u0628\u0631\u0627\u06cc\u0646 \u0645\u06cc\u0632\u0627\u0646 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u0628\u0647 \u0635\u0648\u0631\u062a \u0646\u0645\u0627\u06cc\u06cc \u0627\u0641\u0632\u0627\u06cc\u0634 \u067e\u06cc\u062f\u0627 \u0645\u06cc \u06a9\u0646\u062f.<\/p>\n<p><strong>\u0641\u0627\u0632 \u0633\u0648\u0645 \/ Log linear phase:<\/strong> \u06a9\u0647 \u062a\u0631\u06a9\u06cc\u0628\u0627\u062a \u0648\u0627\u06a9\u0646\u0634 \u0648 \u06a9\u0627\u0631\u0627\u06cc\u06cc \u0622\u0646 \u0647\u0627 \u0631\u0627 \u0628\u0647 \u0627\u062a\u0645\u0627\u0645 \u0627\u0633\u062a.<\/p>\n<p><strong>\u0641\u0627\u0632 \u0686\u0647\u0627\u0631\u0645 \/ <\/strong><strong>plateau phase<\/strong><strong> : <\/strong>\u062f\u0631 \u0627\u06cc\u0646 \u0641\u0627\u0632\u060c \u062a\u0631\u06a9\u06cc\u0628\u0627\u062a \u0648\u0627\u06a9\u0646\u0634 \u0627\u0632 \u0628\u06cc\u0646 \u0645\u06cc \u0631\u0648\u0646\u062f \u0648 \u062f\u06cc\u06af\u0631 \u0645\u062d\u0635\u0648\u0644\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0646\u0645\u06cc \u0634\u0648\u062f \u0628\u0647 \u0647\u0645\u06cc\u0646 \u062e\u0627\u0637\u0631 \u062f\u0631 \u0645\u06cc\u0632\u0627\u0646 \u0641\u0644\u0648\u0631\u0633\u0646\u062a \u0647\u06cc\u0686 \u0627\u0641\u0632\u0627\u06cc\u0634\u06cc \u062f\u06cc\u062f\u0647 \u0646\u0645\u06cc \u0634\u0648\u062f \u0648 \u0628\u0647 \u0627\u0635\u0637\u0644\u0627\u062d \u0631\u06cc\u0644 \u062a\u0627\u06cc\u0645 \u062f\u0631 \u0627\u06cc\u0646 \u0645\u0631\u062d\u0644\u0647 \u0627\u0634\u0628\u0627\u0639 \u0634\u062f\u0647 \u0627\u0633\u062a.<\/p>\n<p>\u0645\u0639\u0645\u0648\u0644\u0627 \u0628\u0647 \u0635\u0648\u0631\u062a \u062a\u0648\u0627\u0641\u0642\u06cc \u0633\u06cc\u06a9\u0644\u06cc \u06a9\u0647 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062f\u0631 \u0622\u0646 \u0648\u0627\u0631\u062f \u0641\u0627\u0632 exponential\u00a0 \u0645\u06cc \u0634\u0648\u0646\u062f \u0648\u00a0 \u0633\u06cc\u06af\u0646\u0627\u0644 \u0648\u0627\u0642\u0639\u06cc \u0627\u0632 noise\u00a0 \u067e\u0633\u200c\u0632\u0645\u06cc\u0646\u0647 \u0642\u0627\u0628\u0644 \u062a\u0634\u062e\u06cc\u0635 \u0627\u0633\u062a \u0631\u0627 \u0633\u06cc\u06a9\u0644 \u0645\u0631\u062c\u0639 \u0648 \u062f\u0631 \u0627\u0635\u0637\u0644\u0627\u062d \u00a0cycle threshold \u0648 \u06cc\u0627 CT\u00a0\u0646\u0627\u0645\u06cc\u062f\u0647 \u0645\u06cc\u200c\u0634\u0648\u062f.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/>\u00a0<a href=\"http:\/\/darwino.ir\/realtime-pcr\/\">\u062a\u06a9\u0646\u06cc\u06a9\/\u0631\u0648\u0634 RealTime PCR \u2013 \u0628\u062e\u0634 \u0627\u0648\u0644 (\u0627\u0646\u0648\u0627\u0639 \u0631\u0648\u0634\u0647\u0627\u06cc \u0622\u0632\u0645\u0627\u06cc\u0634)<\/a><\/strong><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/>\u062a\u06a9\u0646\u06cc\u06a9 Real-Time PCR &#8211; \u0628\u062e\u0634 \u062f\u0648\u0645 (\u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a)<\/strong><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><a href=\"http:\/\/darwino.ir\/realtime-pcr-technique-part-3\/\"><strong>\u062a\u06a9\u0646\u06cc\u06a9 RealTime PCR \u2013 \u0628\u062e\u0634 \u0633\u0648\u0645(\u0645\u0631\u0627\u062d\u0644 \u0627\u0646\u062c\u0627\u0645 \u0648\u0627\u06a9\u0646\u0634)<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><a href=\"http:\/\/darwino.ir\/real-time-pcr-part-iv\/\"><strong>\u062a\u06a9\u0646\u06cc\u06a9 Real time PCR \u2013 \u0628\u062e\u0634 \u0686\u0647\u0627\u0631\u0645(\u0686\u0631\u062e\u0647 \u0627\u0646\u062c\u0627\u0645 \u0648\u0627\u06a9\u0646\u0634)<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><a href=\"http:\/\/darwino.ir\/realtime-pcr-reproduction-curves\/\">\u062a\u06a9\u06cc\u0646\u06a9 RealTime PCR &#8211; \u0628\u062e\u0634 \u067e\u0646\u062c\u0645(\u0627\u0634\u06a9\u0627\u0644\u0627\u062a \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 -\u06f1)<\/a><\/strong><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><a href=\"http:\/\/darwino.ir\/real-time-pcr-technique-checking-controls-and-sample\/\">\u062a\u06a9\u06cc\u0646\u06a9 RealTime PCR -\u0628\u062e\u0634 \u0634\u0634\u0645(\u0627\u0634\u06a9\u0627\u0644\u0627\u062a \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631-\u06f2)<\/a><\/strong><\/h4>\n","protected":false},"excerpt":{"rendered":"<p>\u270d \u0633\u06cc\u06a9\u0644 \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u062f\u0631 \u0631\u0648\u0634 \u0633\u0627\u06cc\u0628\u0631\u06af\u0631\u06cc\u0646 RealTime PCR \u0646\u0648\u0639\u06cc [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":18917,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[30],"tags":[635,532,636,564,3283,779,1443,1445,808,1490],"blog-category":[3070,3071],"darwinoplus-category":[],"class_list":{"0":"post-4117","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","6":"hentry","7":"category-blog","8":"tag-sybr_green","9":"tag-_real_time_pcr","10":"tag-___","14":"tag-___real_time_pcr","15":"tag-_pdf__real_time_pcr","16":"tag-____pcr","17":"tag-____real_time_pcr","18":"blog-category-genetic","19":"blog-category-molecular"},"_links":{"self":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/4117","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/comments?post=4117"}],"version-history":[{"count":13,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/4117\/revisions"}],"predecessor-version":[{"id":21481,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/4117\/revisions\/21481"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media\/18917"}],"wp:attachment":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media?parent=4117"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/categories?post=4117"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/tags?post=4117"},{"taxonomy":"blog-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/blog-category?post=4117"},{"taxonomy":"darwinoplus-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/darwinoplus-category?post=4117"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}