{"id":3551,"date":"2019-06-26T02:27:18","date_gmt":"2019-06-26T02:27:18","guid":{"rendered":"http:\/\/darwino.ir\/blogs\/?p=3551"},"modified":"2020-03-28T11:09:23","modified_gmt":"2020-03-28T07:39:23","slug":"realtime-pcr","status":"publish","type":"post","link":"http:\/\/darwino.ir\/blogs\/realtime-pcr\/","title":{"rendered":"\u062a\u06a9\u0646\u06cc\u06a9\/\u0631\u0648\u0634 RealTime PCR &#8211; \u0628\u062e\u0634 \u0627\u0648\u0644 (\u0627\u0646\u0648\u0627\u0639 \u0631\u0648\u0634\u0647\u0627\u06cc \u0622\u0632\u0645\u0627\u06cc\u0634)"},"content":{"rendered":"<h2><strong>\u270d <\/strong><strong>\u00a0<\/strong><strong>Realtime PCR <\/strong><strong>\u0686\u06cc\u0633\u062a \u0648 \u0686\u0647 \u062a\u0641\u0627\u0648\u062a \u0647\u0627\u06cc\u06cc \u0628\u0627 <\/strong><strong>PCR <\/strong><strong>\u0639\u0627\u062f\u06cc \u062f\u0627\u0631\u062f\u061f<\/strong><\/h2>\n<p><strong>\u062a\u06a9\u0646\u06cc\u06a9 RealTime PCR\u00a0 \u06a9\u0647 \u0628\u0647 \u0622\u0646 Quantitative PCR \u06cc\u0627 PCR\u06a9\u0645\u06cc \u0646\u06cc\u0632\u00a0 \u0645\u06cc \u06af\u0648\u06cc\u0646\u062f \u0628\u0647 \u0637\u0648\u0631 \u0639\u0645\u062f\u0647 \u062f\u0631 \u0628\u0631\u0631\u0633\u06cc \u0647\u0627\u06cc \u06a9\u0645\u06cc \u0628\u06cc\u0627\u0646 \u0698\u0646 \u0628\u0647 \u06a9\u0627\u0631 \u0645\u06cc \u0631\u0648\u062f \u0648\u0644\u06cc \u062f\u0631 \u062a\u0639\u06cc\u06cc\u0646 \u0645\u06cc\u0632\u0627\u0646 \u0648\u06cc\u0631\u0648\u0633 \u0647\u0627\u06cc \u06cc\u06a9 \u0646\u0645\u0648\u0646\u0647 \u0647\u0645 \u062a\u06a9\u0646\u06cc\u06a9\u06cc \u0642\u0648\u06cc \u0648 \u062d\u0633\u0627\u0633 \u0628\u0647 \u0634\u0645\u0627\u0631 \u0645\u06cc \u0622\u06cc\u062f\u060c \u0627\u0644\u0628\u062a\u0647 \u0627\u06cc\u0646 \u062a\u06a9\u0646\u06cc\u06a9 \u06a9\u0627\u0631\u0628\u0631\u062f\u0647\u0627\u06cc \u0641\u0631\u0627\u0648\u0627\u0646\u06cc \u062f\u06cc\u06af\u0631\u06cc \u0647\u0645 \u0686\u0648\u0646 \u0628\u0631\u0631\u0633\u06cc \u0645\u06cc\u0632\u0627\u0646 \u062a\u0623\u062b\u06cc\u0631 \u062f\u0627\u0631\u0648 \u062f\u0631\u0645\u0627\u0646\u06cc\u060c \u0633\u0646\u062c\u0634 \u0622\u0633\u06cc\u0628 \u0647\u0627\u06cc DNA\u060c \u062a\u0634\u062e\u06cc\u0635 \u0639\u0648\u0627\u0645\u0644 \u0628\u06cc\u0645\u0627\u0631\u06cc \u0632\u0627\u060c \u062a\u0639\u06cc\u06cc\u0646 \u0698\u0646\u0648\u062a\u06cc\u067e \u0627\u0641\u0631\u0627\u062f\u060c \u0633\u0646\u062c\u0634 \u062a\u0641\u06a9\u06cc\u06a9 \u0634\u062f\u0646 \u0622\u0644\u0644\u06cc \u0648 &#8230; \u062f\u0627\u0631\u062f.<\/strong><\/p>\n<p>\u062f\u0631 \u0627\u06cc\u0646 \u0631\u0648\u0634 \u0627\u0632 \u06cc\u06a9 \u0645\u0648\u0644\u06a9\u0648\u0644 \u06af\u0632\u0627\u0631\u0634\u06af\u0631 \u0641\u0644\u0648\u0631\u0648\u0633\u0646\u062a \u0628\u0631\u0627\u06cc \u0645\u0634\u0627\u0647\u062f\u0647 \u067e\u06cc\u0634\u0631\u0641\u062a PCR \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f \u0648 \u0642\u062f\u0631\u062a \u0633\u06cc\u06af\u0646\u0627\u0644 \u0641\u0644\u0648\u0626\u0648\u0631\u0633\u0646\u062a \u062a\u0648\u0644\u06cc\u062f \u0634\u062f\u0647 \u0627\u0631\u062a\u0628\u0627\u0637 \u0645\u0633\u062a\u0642\u06cc\u0645\u06cc \u0628\u0627 \u0645\u0642\u062f\u0627\u0631 \u0645\u0648\u0644\u06a9\u0648\u0644\u200c\u0647\u0627\u06cc \u062a\u06a9\u062b\u06cc\u0631\u0634\u062f\u0647 \u062f\u0627\u0631\u062f. \u062f\u0631 \u0627\u06cc\u0646 \u062a\u06a9\u0646\u06cc\u06a9 \u0645\u06cc\u0632\u0627\u0646 \u0645\u062d\u0635\u0648\u0644\u0627\u062a\u06cc \u06a9\u0647 \u062d\u06cc\u0646 \u06cc\u06a9 \u0622\u0632\u0645\u0627\u06cc\u0634 <a href=\"http:\/\/darwino.ir\/pcr-technique\/\"><strong>PCR<\/strong> <\/a>\u062a\u0648\u0644\u06cc\u062f \u0645\u06cc\u200c\u0634\u0648\u0646\u062f \u0628\u0627 \u0645\u06cc\u0632\u0627\u0646 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062a\u0648\u0644\u06cc\u062f \u0634\u062f\u0647 \u0637\u06cc \u0622\u0632\u0645\u0627\u06cc\u0634\u200c\u0647\u0627\u06cc PCR \u0628\u0627 \u0645\u0642\u062f\u0627\u0631 \u0646\u0648\u06a9\u0644\u0626\u06cc\u06a9 \u200c\u0627\u0633\u06cc\u062f \u0622\u063a\u0627\u0632\u06a9\u0646\u0646\u062f\u0647 \u0645\u0634\u062e\u0635\u060c \u0645\u0642\u0627\u06cc\u0633\u0647 \u0645\u06cc\u200c\u0634\u0648\u062f \u0648 \u0627\u0645\u06a9\u0627\u0646 \u067e\u06cc \u0628\u0631\u062f\u0646 \u0628\u0647 \u0645\u0642\u062f\u0627\u0631 \u0646\u0648\u06a9\u0644\u0626\u06cc\u06a9 \u0627\u0633\u06cc\u062f \u0627\u0648\u0644\u06cc\u0647 \u0645\u0648\u062c\u0648\u062f \u062f\u0631 \u0646\u0645\u0648\u0646\u0647\u060c \u0628\u0631\u0627\u06cc \u0645\u0627 \u0641\u0631\u0627\u0647\u0645 \u0645\u06cc \u0634\u0648\u062f.<\/p>\n<h4><strong>\u0628\u0631 \u0645\u0628\u0646\u0627\u06cc \u0645\u0644\u06a9\u0648\u0644\u06cc \u06a9\u0647 \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f \u0627\u06cc\u0646 \u062a\u06a9\u0646\u06cc\u06a9 \u0628\u0647 \u062f\u0648 \u0631\u0648\u0634 \u062a\u0642\u0633\u06cc\u0645 \u0628\u0646\u062f\u06cc \u0645\u06cc \u0634\u0648\u062f<\/strong><strong>:<\/strong><\/h4>\n<p><strong>\u00a0\u06f1-<\/strong><strong>\u062a\u0634\u062e\u06cc\u0635 \u063a\u06cc\u0631 \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u0631\u0646\u06af \u0647\u0627\u06cc \u0628\u0627\u0646\u062f \u0634\u062f\u0647 \u0628\u0647 <\/strong><strong>DNA<\/strong><\/p>\n<p>\u062f\u0631 \u0627\u06cc\u0646\u062c\u0627 \u0627\u0632 \u0631\u0646\u06af \u0647\u0627\u06cc \u0628\u0627\u0646\u062f \u0634\u062f\u0647 \u0628\u0647 DNA \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u06af\u0632\u0627\u0631\u0634\u06af\u0631 \u0641\u0644\u0648\u0631\u0648\u0633\u0646\u062a \u0628\u0631\u0627\u06cc \u0645\u0634\u0627\u0647\u062f\u0647 \u0648\u0627\u06a9\u0646\u0634 PCR \u0627\u0633\u062a\u0641\u0627\u062f\u0647 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\u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u0622\u0646 \u0645\u0639\u0645\u0648\u0644\u200c\u062a\u0631 \u0627\u0633\u062a. \u0627\u06cc\u0646 \u062a\u06a9\u0646\u06cc\u06a9 \u0628\u0647 Taq Man\u02ca \u06f5 nuclease assay \u0645\u0639\u0631\u0648\u0641 \u0627\u0633\u062a. \u067e\u0631\u0648\u0628 \u0628\u0647 \u06a9\u0627\u0631 \u0631\u0641\u062a\u0647 \u062f\u0631 \u0627\u06cc\u0646 \u0641\u0631\u0627\u06cc\u0646\u062f \u0627\u0632 \u0622\u0646 \u062c\u0647\u062a reporter \u0646\u0627\u0645\u06cc\u062f\u0647 \u0634\u062f\u0647 \u06a9\u0647 \u0646\u0634\u0627\u0646\u06af\u0631 \u0648\u062c\u0648\u062f \u0645\u062d\u0635\u0648\u0644 \u0645\u0648\u0631\u062f\u0646\u0638\u0631 \u0627\u0633\u062a. \u0645\u062d\u0644 \u0627\u062a\u0635\u0627\u0644 \u067e\u0631\u0648\u0628\u060c \u0628\u0647 \u0631\u0634\u062a\u0647 \u0627\u0644\u06af\u0648 \u0648 \u0646\u0632\u062f\u06cc\u06a9 \u0628\u0647 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc \u0627\u0635\u0644\u06cc PCR \u0648 \u067e\u0627\u06cc\u06cc\u0646\u200c\u062a\u0631 \u0627\u0632 \u0622\u0646 \u0627\u0633\u062a. \u0627\u06cc\u0646 \u0645\u062a\u062f \u06a9\u0645\u062a\u0631 \u062f\u0631 \u0645\u0639\u0631\u0636 \u062e\u0637\u0627\u0647\u0627\u06cc\u06cc \u0642\u0631\u0627\u0631 \u062f\u0627\u0631\u062f \u06a9\u0647 \u0627\u0632 \u0627\u062a\u0635\u0627\u0644\u0627\u062a \u0646\u0627\u062f\u0631\u0633\u062a \u0627\u0632 \u062c\u0645\u0644\u0647 \u062f\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc \u067e\u0631\u0627\u06cc\u0645\u0631 \u062d\u0627\u0635\u0644 \u0645\u06cc\u200c\u0634\u0648\u062f. \u062f\u0631 \u0627\u06cc\u0646 \u062d\u0627\u0644\u062a \u0647\u0631 \u0645\u0648\u0644\u06a9\u0648\u0644 \u067e\u0631\u0648\u0628 \u0645\u062a\u0634\u06a9\u0644 \u0627\u0632 \u06cc\u06a9 \u0627\u0644\u06cc\u06af\u0648\u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f \u0648 \u062f\u0648 \u0644\u06cc\u0628\u0644 \u0627\u0633\u062a. \u0631\u0646\u06af \u0641\u0644\u0648\u0626\u0648\u0631\u0648\u0633\u0646\u062a \u0628\u0647 \u0627\u0646\u062a\u0647\u0627\u06cc \u064e\u06f5 \u0631\u0634\u062a\u0647 \u0627\u0644\u06cc\u06af\u0648\u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f\u06cc \u0648 \u062a\u0631\u06a9\u06cc\u0628 quencher \u0628\u0647 \u0627\u0646\u062a\u0647\u0627\u06cc \u062f\u06cc\u06af\u0631 \u0631\u0634\u062a\u0647 \u0645\u062a\u0635\u0644 \u0645\u06cc\u200c\u0634\u0648\u062f. \u0627\u06cc\u0646 \u062a\u0631\u06a9\u06cc\u0628 \u0648\u0638\u06cc\u0641\u0647 \u0645\u0647\u0627\u0631 \u0633\u06cc\u06af\u0646\u0627\u0644 \u0641\u0644\u0648\u0626\u0648\u0631\u0648\u0633\u0646\u062a \u0631\u0627 \u0628\u0631 \u0639\u0647\u062f\u0647 \u062f\u0627\u0631\u062f \u0648 \u0627\u06cc\u0646 \u06a9\u0627\u0631 \u0631\u0627 \u0628\u0627 \u062c\u0630\u0628 \u0627\u0646\u0631\u0698\u06cc \u0627\u0632 \u0631\u0646\u06af \u0641\u0644\u0648\u0626\u0648\u0631\u0648\u0633\u0646\u062a \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc\u200c\u062f\u0647\u062f. \u0627\u06cc\u0646 \u067e\u062f\u06cc\u062f\u0647 <strong>FRET<\/strong> (<strong>Fluorescence Resonance Energy Transfer) <\/strong>\u0646\u0627\u0645 \u062f\u0627\u0631\u062f. \u062f\u0631 \u062d\u0627\u0644\u062a \u0639\u0627\u062f\u06cc \u0633\u06cc\u06af\u0646\u0627\u0644 \u0641\u0644\u0648\u0626\u0648\u0631\u0648\u0633\u0646\u062a\u06cc \u0627\u06cc\u062c\u0627\u062f \u0646\u0645\u06cc\u200c\u0634\u0648\u062f\u061b \u0686\u0648\u0646 \u0627\u0644\u06cc\u06af\u0648\u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f \u0628\u0647 \u06af\u0648\u0646\u0647\u200c\u0627\u06cc \u0637\u0631\u0627\u062d\u06cc \u0634\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 \u062f\u0648 \u0627\u0646\u062a\u0647\u0627\u06cc \u0622\u0646 \u0628\u0627 \u0647\u0645\u062f\u06cc\u06af\u0631 \u0645\u06a9\u0645\u0644 \u0634\u062f\u0647 \u0648 \u0631\u0646\u06af \u0641\u0644\u0648\u0626\u0648\u0631\u0648\u0633\u0646\u062a \u0648 \u062a\u0631\u06a9\u06cc\u0628 quencher \u062f\u0631 \u06a9\u0646\u0627\u0631 \u0647\u0645\u062f\u06cc\u06af\u0631 \u0642\u0631\u0627\u0631 \u0645\u06cc\u200c\u06af\u06cc\u0631\u0646\u062f. \u0647\u06cc\u0628\u0631\u06cc\u062f\u0627\u0633\u06cc\u0648\u0646 \u0628\u06cc\u0646 \u0627\u0644\u06cc\u06af\u0648\u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f \u0648 \u0645\u062d\u0635\u0648\u0644 PCR\u060c \u0627\u062a\u0635\u0627\u0644 \u0628\u06cc\u0646 \u062f\u0648\u0633\u0631 \u0627\u0644\u06cc\u06af\u0648\u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f \u0631\u0627 \u0628\u0631 \u0647\u0645 \u0645\u06cc\u200c\u0632\u0646\u062f \u0648 \u0627\u06cc\u0646 \u06a9\u0627\u0631 \u0628\u0627\u0639\u062b \u0627\u06cc\u062c\u0627\u062f \u0633\u06cc\u06af\u0646\u0627\u0644 \u0641\u0644\u0648\u0626\u0648\u0631\u0648\u0633\u0646\u062a \u0645\u06cc\u200c\u06af\u0631\u062f\u062f. \u062f\u0631 \u062d\u06cc\u0646 \u0627\u0646\u062c\u0627\u0645\u200c \u0634\u062f\u0646 PCR\u060c <strong>\u067e\u0644\u06cc\u0645\u0631\u0627\u0632 Taq<\/strong>\u060c \u0628\u0627 \u0631\u0633\u06cc\u062f\u0646 \u0628\u0647 \u067e\u0631\u0648\u0628 \u0628\u0627 \u062e\u0627\u0635\u06cc\u062a \u0627\u06af\u0632\u0648\u0646\u0648\u06a9\u0644\u0626\u0627\u0632\u06cc \u06f3\u064e \u0628\u0647\u00a0 \u06f5\u064e \u062e\u0648\u062f \u0622\u0646 \u0631\u0627 \u062a\u062c\u0632\u06cc\u0647 \u0645\u06cc\u200c\u06a9\u0646\u062f \u0648 \u0628\u0627 \u0627\u06cc\u0646 \u06a9\u0627\u0631 \u0631\u0646\u06af \u0648 \u062a\u0631\u06a9\u06cc\u0628 quencher \u062f\u0631 \u0645\u062d\u06cc\u0637 \u0622\u0632\u0627\u062f \u0645\u06cc\u200c\u06af\u0631\u062f\u0646\u062f. \u0647\u0631 \u0686\u0647 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062a\u0648\u0644\u06cc\u062f \u0634\u062f\u0647 \u0628\u06cc\u0634\u062a\u0631 \u0628\u0627\u0634\u0646\u062f\u060c \u067e\u0631\u0648\u0628\u200c\u0647\u0627\u06cc \u0628\u06cc\u0634\u062a\u0631\u06cc \u0628\u0647 \u062a\u0648\u0627\u0644\u06cc \u0645\u06a9\u0645\u0644 \u062e\u0648\u062f \u062f\u0631 \u0627\u06cc\u0646 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u0645\u062a\u0635\u0644 \u0645\u06cc\u200c\u0634\u0648\u0646\u062f. \u0647\u0631 \u0686\u0647 \u062a\u0639\u062f\u0627\u062f \u067e\u0631\u0648\u0628 \u0628\u06cc\u0634\u062a\u0631\u06cc \u0645\u062a\u0635\u0644 \u0634\u0648\u062f\u060c \u0633\u06cc\u06af\u0646\u0627\u0644 \u0641\u0644\u0648\u0626\u0648\u0631\u0648\u0633\u0646\u062a \u0628\u06cc\u0634\u062a\u0631\u06cc \u062d\u06cc\u0646 \u0622\u0632\u0627\u062f \u0634\u062f\u0646 \u0631\u0646\u06af \u0627\u0632 quencher \u0627\u06cc\u062c\u0627\u062f \u0645\u06cc\u200c\u06af\u0631\u062f\u062f. \u062f\u0631\u0646\u062a\u06cc\u062c\u0647 \u0627\u0631\u062a\u0628\u0627\u0637\u06cc \u0645\u06cc\u0627\u0646 \u0633\u06cc\u06af\u0646\u0627\u0644\u200c\u0647\u0627\u06cc \u0641\u0644\u0648\u0626\u0648\u0631\u0648\u0633\u0646\u062a \u0627\u06cc\u062c\u0627\u062f \u0634\u062f\u0647 \u062f\u0631 Real-time PCR \u0648 \u0645\u0642\u062f\u0627\u0631 \u062a\u0648\u0627\u0644\u06cc \u0627\u0644\u06af\u0648 \u0648\u062c\u0648\u062f \u062f\u0627\u0631\u062f.<\/p>\n<p>&nbsp;<\/p>\n<h4 class=\"entry-title fusion-post-title\"><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/> \u062a\u06a9\u0646\u06cc\u06a9\/\u0631\u0648\u0634 RealTime PCR \u2013 \u0628\u062e\u0634 \u0627\u0648\u0644 (\u0627\u0646\u0648\u0627\u0639 \u0631\u0648\u0634\u0647\u0627\u06cc \u0622\u0632\u0645\u0627\u06cc\u0634)<\/strong><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><a href=\"http:\/\/darwino.ir\/real-time-pcr-section-2\/\"><strong>\u062a\u06a9\u0646\u06cc\u06a9 Real-Time PCR \u2013 \u0628\u062e\u0634 \u062f\u0648\u0645 (\u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a)<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><a href=\"http:\/\/darwino.ir\/realtime-pcr-technique-part-3\/\"><strong>\u062a\u06a9\u0646\u06cc\u06a9 RealTime PCR \u2013 \u0628\u062e\u0634 \u0633\u0648\u0645(\u0645\u0631\u0627\u062d\u0644 \u0627\u0646\u062c\u0627\u0645 \u0648\u0627\u06a9\u0646\u0634)<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><a href=\"http:\/\/darwino.ir\/real-time-pcr-part-iv\/\"><strong>\u062a\u06a9\u0646\u06cc\u06a9 Real time PCR \u2013 \u0628\u062e\u0634 \u0686\u0647\u0627\u0631\u0645(\u0686\u0631\u062e\u0647 \u0627\u0646\u062c\u0627\u0645 \u0648\u0627\u06a9\u0646\u0634)<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><a href=\"http:\/\/darwino.ir\/realtime-pcr-reproduction-curves\/\">\u062a\u06a9\u06cc\u0646\u06a9 RealTime PCR &#8211; \u0628\u062e\u0634 \u067e\u0646\u062c\u0645(\u0627\u0634\u06a9\u0627\u0644\u0627\u062a \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 -\u06f1)<\/a><\/strong><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><a href=\"http:\/\/darwino.ir\/real-time-pcr-technique-checking-controls-and-sample\/\">\u062a\u06a9\u06cc\u0646\u06a9 RealTime PCR -\u0628\u062e\u0634 \u0634\u0634\u0645(\u0627\u0634\u06a9\u0627\u0644\u0627\u062a \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631-\u06f2)<\/a><\/strong><\/h4>\n","protected":false},"excerpt":{"rendered":"<p>\u270d \u00a0Realtime PCR \u0686\u06cc\u0633\u062a \u0648 \u0686\u0647 \u062a\u0641\u0627\u0648\u062a \u0647\u0627\u06cc\u06cc \u0628\u0627 PCR [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":3552,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[30],"tags":[3281,3280,635,3282,669,532,564,779,1443,1549,1445,1548,1490,1547,668],"blog-category":[3070,3071],"darwinoplus-category":[],"class_list":{"0":"post-3551","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","6":"hentry","7":"category-blog","8":"tag-fluorescence-resonance_energy_transfer","9":"tag-quantitative_pcr","10":"tag-sybr_green","11":"tag-_taq","12":"tag-_real_time_pcr__pcr_","13":"tag-_real_time_pcr","14":"tag-___","16":"tag-___real_time_pcr","17":"tag--__real_time_pcr","18":"tag-_pdf__real_time_pcr","20":"tag-____real_time_pcr","21":"tag--___real_time_pcr","22":"tag-__cdna","23":"blog-category-genetic","24":"blog-category-molecular"},"_links":{"self":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/3551","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/comments?post=3551"}],"version-history":[{"count":21,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/3551\/revisions"}],"predecessor-version":[{"id":21480,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/3551\/revisions\/21480"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media\/3552"}],"wp:attachment":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media?parent=3551"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/categories?post=3551"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/tags?post=3551"},{"taxonomy":"blog-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/blog-category?post=3551"},{"taxonomy":"darwinoplus-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/darwinoplus-category?post=3551"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}