{"id":22045,"date":"2020-04-08T09:26:41","date_gmt":"2020-04-08T05:56:41","guid":{"rendered":"http:\/\/darwino.ir\/blogs\/?p=22045"},"modified":"2020-12-14T09:55:53","modified_gmt":"2020-12-14T06:25:53","slug":"sequencing-by-oligonucleotide-ligation-and-detection","status":"publish","type":"post","link":"http:\/\/darwino.ir\/blogs\/sequencing-by-oligonucleotide-ligation-and-detection\/","title":{"rendered":"\u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0628\u0647 \u0631\u0648\u0634 SOLiD (Sequencing by Oligonucleotide Ligation and Detection)"},"content":{"rendered":"

\u270d<\/strong>\u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc SOLiD \u06cc\u06a9\u06cc \u0627\u0632 \u0631\u0648\u0634 \u0647\u0627\u06cc\/ \u062a\u06a9\u0646\u06cc\u06a9 \u0647\u0627\u06cc \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0646\u0633\u0644 \u062f\u0648\u0645 (NGS)\u00a0<\/strong><\/h2>\n

 <\/p>\n

\u062a\u06a9\u0646\u0648\u0644\u0648\u0698\u06cc SOLiD \u062f\u0631 \u0633\u0627\u0644 \u06f2\u06f0\u06f0\u06f6 \u0627\u0631\u0627\u0626\u0647 \u06af\u0631\u062f\u06cc\u062f \u0648 \u062f\u0631 \u0627\u0648\u0627\u062e\u0631 \u0633\u0627\u0644 \u06f2\u06f0\u06f0\u06f7 \u062a\u0648\u0633\u0637 \u0634\u0631\u06a9\u062a ABI (Applied Bioscience)<\/strong> <\/a>\u062e\u0631\u06cc\u062f\u0627\u0631\u06cc \u0634\u062f \u0648 \u0628\u0647 \u0635\u0648\u0631\u062a \u062a\u062c\u0627\u0631\u06cc \u0639\u0631\u0636\u0647 \u0634\u062f. \u062f\u0631 \u0631\u0648\u0634 SOLiD \u0647\u0645\u0627\u0646\u0646\u062f \u0631\u0648\u0634 \u067e\u0627\u06cc\u0631\u0648\u0633\u06a9\u0626\u0648\u0646\u0633\u06cc\u0646\u06af (Pyrosequencing) \u062f\u0631 \u0627\u0628\u062a\u062f\u0627 \u06a9\u062a\u0627\u0628\u062e\u0627\u0646\u0647 DNA \u062a\u0647\u06cc\u0647 \u0634\u062f\u0647 \u0648 \u0633\u067e\u0633 \u062a\u06a9\u062b\u06cc\u0631 \u0642\u0637\u0639\u0627\u062a DNA \u0628\u0631 \u0631\u0648\u06cc \u062f\u0627\u0646\u0647 \u0647\u0627\u06cc \u067e\u0648\u0634\u06cc\u062f\u0647 \u0627\u0632 \u0622\u063a\u0627\u0632\u06af\u0631 \u06a9\u0647 \u0645\u0647\u0631\u0647 \u0647\u0627\u06cc \u067e\u0627\u0631\u0627\u0645\u063a\u0646\u0627\u0637\u06cc\u0633\u06cc \u06f1\u03bc\u039c<\/strong> \u0647\u0633\u062a\u0646\u062f \u0628\u0627 \u0631\u0648\u0634 emPCR<\/strong> (emulsion PCR)<\/strong> \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u06af\u06cc\u0631\u062f. \u0633\u067e\u0633 \u0647\u0631 \u06cc\u06a9 \u0627\u0632 \u0627\u06cc\u0646 \u0645\u0647\u0631\u0647 \u0647\u0627 \u0631\u0648\u06cc \u0633\u0637\u062d \u0634\u06cc\u0634\u0647 \u0627\u06cc (SOLiD flow well) \u0628\u0647 \u0637\u0648\u0631 \u062a\u0635\u0627\u062f\u0641\u06cc \u062a\u0648\u0632\u06cc\u0639 \u0634\u062f\u0647 \u0648 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0628\u0647 \u0648\u0633\u06cc\u0644\u0647 \u0622\u0646\u0632\u06cc\u0645 \u0644\u06cc\u06af\u0627\u0632 \u0628\u0647 \u062c\u0627\u06cc \u067e\u0644\u06cc\u0645\u0631\u0627\u0632 (Sequencing by Ligation) \u0635\u0648\u0631\u062a \u0645\u06cc \u06af\u06cc\u0631\u062f. \u0628\u0631\u0627\u06cc \u0627\u06cc\u0646 \u06a9\u0627\u0631 \u0627\u0628\u062a\u062f\u0627 \u0622\u063a\u0627\u0632\u06af\u0631 \u0639\u0645\u0648\u0645\u06cc \u0628\u0647 \u06cc\u06a9\u06cc \u0627\u0632 \u0633\u0627\u0632\u06af\u0627\u0631 \u062f\u0647\u0646\u062f\u0647 \u0647\u0627 \u062c\u0641\u062a \u0634\u062f\u0647 \u0648 \u067e\u0633 \u0627\u0632 \u0622\u0646 \u06a9\u0627\u0648\u0634\u06af\u0631\u0647\u0627\u06cc \u0641\u0644\u0648\u0631\u0633\u0646\u062a \u06f8 \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f\u06cc \u0628\u0647 \u0645\u0646\u0638\u0648\u0631 \u062c\u0641\u062a \u0634\u062f\u0646 \u0628\u0627 \u0642\u0637\u0639\u0627\u062a \u062d\u0627\u0635\u0644 \u0627\u0632 \u062a\u06a9\u062b\u06cc\u0631 \u062f\u0631 \u0645\u062c\u0627\u0648\u0631\u062a \u0622\u063a\u0627\u0632\u06af\u0631 \u0639\u0645\u0648\u0645\u06cc \u0628\u0627 \u06cc\u06a9\u062f\u06cc\u06af\u0631 \u0631\u0642\u0627\u0628\u062a \u0645\u06cc \u06a9\u0646\u0646\u062f.<\/p>\n

\u06a9\u0627\u0648\u0634\u06af\u0631\u0647\u0627\u06cc \u0627\u0644\u06cc\u06af\u0648 \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f\u06cc \u062f\u0627\u0631\u0627\u06cc \u062c\u0627\u06cc\u06af\u0627\u0647 \u0627\u062a\u0635\u0627\u0644 \u062f\u0631 \u0627\u0648\u0644\u06cc\u0646 \u0628\u0627\u0632\u060c \u062c\u0627\u06cc\u06af\u0627\u0647 \u0628\u0631\u0634 \u062f\u0631 \u067e\u0646\u062c\u0645\u06cc\u0646 \u0628\u0627\u0632 \u0648 \u06f4 \u0631\u0646\u06af \u0641\u0644\u0648\u0631\u0633\u0646\u062a \u0645\u062e\u062a\u0644\u0641 \u0645\u062a\u0635\u0644 \u0628\u0647 \u0622\u062e\u0631\u06cc\u0646 \u0628\u0627\u0632 \u0645\u06cc \u0628\u0627\u0634\u0646\u062f. \u0627\u0632 \u0622\u0646\u062c\u0627 \u06a9\u0647 \u06f2 \u0628\u0627\u0632 \u0627\u0628\u062a\u062f\u0627\u06cc\u06cc \u062a\u0639\u06cc\u06cc\u0646 \u06a9\u0646\u0646\u062f\u0647 \u0646\u0648\u0639 \u0631\u0646\u06af \u0641\u0644\u0648\u0631\u0633\u0646\u062a \u0645\u062a\u0635\u0644 \u0628\u0647 \u06a9\u0627\u0648\u0634\u06af\u0631 \u0628\u0648\u062f\u0647 \u0648 \u0628\u0647 \u0637\u0648\u0631 \u0647\u0645 \u0632\u0645\u0627\u0646 \u06f2 \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f \u0631\u0627 \u0628\u0631\u0631\u0633\u06cc \u0645\u06cc \u06a9\u0646\u0646\u062f \u0628\u0647 \u06a9\u0627\u0648\u0634\u06af\u0631\u0647\u0627 di-base Probes\u00a0 \u06af\u0641\u062a\u0647 \u0645\u06cc \u0634\u0648\u062f. \u0628\u0627 \u062c\u0641\u062a \u0634\u062f\u0646 \u06a9\u0627\u0648\u0634\u06af\u0631\u0647\u0627\u06cc \u0627\u0644\u06cc\u06af\u0648 \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f\u06cc \u0628\u0647 \u0631\u0634\u062a\u0647 \u0627\u0644\u06af\u0648 \u0633\u06cc\u06af\u0646\u0627\u0644 \u0641\u0644\u0648\u0631\u0633\u0646\u062a \u062b\u0628\u062a \u0645\u06cc \u0634\u0648\u062f \u0648 \u0628\u0647 \u0648\u0633\u06cc\u0644\u0647 \u06cc \u0628\u0631\u0634 \u0634\u06cc\u0645\u06cc\u0627\u06cc\u06cc \u0628\u06cc\u0646 \u067e\u0646\u062c\u0645\u06cc\u0646 \u0648 \u0634\u0634\u0645\u06cc\u0646 \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f \u06a9\u0627\u0648\u0634\u06af\u0631 \u0633\u06cc\u06af\u0646\u0627\u0644 \u062d\u0630\u0641 \u0645\u06cc \u0634\u0648\u062f. \u067e\u0633 \u0627\u0632 \u0628\u0631\u0634\u060c \u06a9\u0627\u0648\u0634\u06af\u0631 \u0627\u06a9\u062a\u0627\u0645\u0631 \u062f\u06cc\u06af\u0631\u06cc \u0645\u062a\u0635\u0644 \u0634\u062f\u0647 \u0648 \u0686\u0631\u062e\u0647 \u0627\u062a\u0635\u0627\u0644 \u062c\u062f\u06cc\u062f\u06cc \u0634\u06a9\u0644 \u0645\u06cc \u06af\u06cc\u0631\u062f.<\/p>\n

\u0628\u0627 \u0627\u06cc\u0646 \u0631\u0648\u0646\u062f \u0627\u0632 \u06af\u0631\u0648\u0647 \u067e\u0646\u062c \u062a\u0627\u06cc\u06cc \u06f2 \u0628\u0627\u0632 \u0627\u0628\u062a\u062f\u0627\u06cc\u06cc \u0622\u0646 \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc \u0645\u06cc \u0634\u0648\u062f \u0648 \u0633\u0631\u0627\u0646\u062c\u0627\u0645 \u062a\u0645\u0627\u0645 \u0642\u0637\u0639\u0647 \u0628\u0647 \u0635\u0648\u0631\u062a \u062f\u0648 \u0631\u0634\u062a\u0647 \u0627\u06cc \u062f\u0631 \u0645\u06cc \u0622\u06cc\u062f. \u0633\u067e\u0633 \u0631\u0634\u062a\u0647 \u062a\u0627\u0632\u0647 \u0633\u0646\u062a\u0632 \u0634\u062f\u0647 \u0648 \u0622\u063a\u0627\u0632\u06af\u0631 \u0639\u0645\u0648\u0645\u06cc \u0627\u0632 \u0631\u0634\u062a\u0647 DNA \u0627\u0648\u0644\u06cc\u0647 \u062c\u062f\u0627 \u0645\u06cc \u06af\u0631\u062f\u062f \u0648 \u0622\u063a\u0627\u0632\u06af\u0631 \u0639\u0645\u0648\u0645\u06cc \u062c\u062f\u06cc\u062f\u06cc \u06a9\u0647 \u0628\u0647 \u0627\u0646\u062f\u0627\u0632\u0647 \u06cc\u06a9 \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f \u0627\u0632 \u0622\u063a\u0627\u0632\u06af\u0631 \u0642\u0628\u0644\u06cc \u06a9\u0648\u062a\u0627\u0647 \u062a\u0631 \u0627\u0633\u062a (n_1) \u0627\u0636\u0627\u0641\u0647 \u0645\u06cc \u06af\u0631\u062f\u062f. \u0628\u0647 \u0645\u0646\u0638\u0648\u0631 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u062a\u0645\u0627\u0645\u06cc \u0628\u0627\u0632\u0647\u0627 \u0627\u06cc\u0646 \u0641\u0631\u0627\u06cc\u0646\u062f \u067e\u0646\u062c \u0628\u0627\u0631 \u0648 \u0628\u0627 \u067e\u0646\u062c \u0622\u063a\u0627\u0632\u06af\u0631 \u0639\u0645\u0648\u0645\u06cc \u0628\u0627 \u0627\u0646\u062f\u0627\u0632\u0647 n \u062a\u0627 n_4 \u062a\u06a9\u0631\u0627\u0631 \u0645\u06cc \u0634\u0648\u062f.<\/p>\n

\u062f\u0631 \u0631\u0648\u0634 SOLiD \u0647\u0631 \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f \u0645\u0648\u062c\u0648\u062f \u062f\u0631 \u0647\u0631 \u06cc\u06a9 \u0627\u0632 \u0642\u0637\u0639\u0627\u062a \u06f2 \u0645\u0631\u062a\u0628\u0647\u060c \u062f\u0631 \u06f2 \u0648\u0627\u06a9\u0646\u0634 \u0627\u062a\u0635\u0627\u0644 \u0645\u0633\u062a\u0642\u0644 \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc \u0645\u06cc \u0634\u0648\u062f \u0648 \u062f\u0631 \u0646\u062a\u06cc\u062c\u0647 \u0645\u0647\u0645 \u062a\u0631\u06cc\u0646 \u0645\u0632\u06cc\u062a \u0627\u06cc\u0646 \u0631\u0648\u0634 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0645\u06cc\u0632\u0627\u0646 \u0635\u062d\u062a %\u06f9\u06f9\/\u06f9\u06f9 \u0622\u0646 \u0645\u06cc \u0628\u0627\u0634\u062f\u060c \u0627\u0632 \u0627\u06cc\u0646 \u0631\u0648\u060c \u062f\u0642\u062a \u062a\u0648\u0627\u0644\u06cc \u06cc\u0627\u0628\u06cc \u062f\u0631 \u0627\u06cc\u0646 \u0631\u0648\u0634 \u062f\u0631 \u0645\u0642\u0627\u06cc\u0633\u0647 \u0628\u0627 \u0631\u0648\u0634 \u0647\u0627\u06cc \u062f\u06cc\u06af\u0631 \u062f\u0631 \u0633\u0637\u062d \u0628\u0627\u0644\u0627\u06cc\u06cc \u0627\u0633\u062a. \u0648\u0644\u06cc \u0645\u0647\u0645 \u062a\u0631\u06cc\u0646 \u0645\u062d\u062f\u0648\u062f\u06cc\u062a \u0627\u06cc\u0646 \u0631\u0648\u0634 \u06a9\u0648\u062a\u0627\u0647 \u0628\u0648\u062f\u0646 \u0637\u0648\u0644 \u062a\u0648\u0627\u0644\u06cc \u062e\u0648\u0627\u0646\u062f\u0647 \u0634\u062f\u0647 \u0645\u06cc \u0628\u0627\u0634\u062f \u06a9\u0647 \u06f3\u06f5 \u062a\u0627 \u06f7\u06f5 \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f \u0627\u0633\u062a.<\/p>\n

\u062f\u0631 \u0622\u063a\u0627\u0632\u060c \u062f\u0631 \u0647\u0631 \u0627\u062c\u0631\u0627\u06cc \u062f\u0633\u062a\u06af\u0627\u0647 \u062a\u0627 \u06f3GB \u062a\u0648\u0627\u0644\u06cc \u062e\u0648\u0627\u0646\u062f\u0647 \u0645\u06cc \u0634\u062f\u060c \u0627\u0645\u0627 \u062f\u0631 \u062f\u0633\u0627\u0645\u0628\u0631 \u06f2\u06f0\u06f0\u06f9\u060c \u0646\u0633\u062e\u0647 \u06cc \u062c\u062f\u06cc\u062f\u06cc \u0627\u0632 \u0627\u06cc\u0646 \u062a\u06a9\u0646\u0648\u0644\u0648\u0698\u06cc \u0628\u0627 \u0646\u0627\u0645 Plus 3TM SOLiD<\/strong> \u0645\u0639\u0631\u0641\u06cc \u06af\u0631\u062f\u06cc\u062f \u06a9\u0647 \u0645\u06cc \u062a\u0648\u0627\u0646\u062f \u062f\u0631 \u0647\u0631 \u0627\u062c\u0631\u0627\u060c \u0628\u06cc\u0634 \u0627\u0632 \u06f6\u06f0GB \u062a\u0648\u0627\u0644\u06cc \u0631\u0627 \u0628\u062e\u0648\u0627\u0646\u062f.<\/p>\n

 <\/p>\n

\"Internet<\/strong>\u00a0\u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0633\u0646\u06af\u0631 \u0628\u0647 \u0631\u0648\u0634 \u0627\u062a\u0648\u0645\u0627\u062a<\/a><\/strong><\/h4>\n

\"Internet<\/strong>\u062a\u06a9\u0646\u0648\u0644\u0648\u0698\u06cc\/\u0631\u0648\u0634 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0627\u06cc\u0644\u0648\u0645\u06cc\u0646\u0627<\/strong> (Illumina)<\/strong><\/a><\/h4>\n

\"Internet\u00a0\u00a0<\/strong>\u0631\u0648\u0634 \u0633\u0646\u06af\u0631 (sanger) \u2013 \u0646\u0633\u0644 \u0627\u0648\u0644 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc DNA<\/strong><\/a><\/h4>\n

\"Internet<\/strong>\u062a\u0648\u0627\u0644\u06cc \u06cc\u0627\u0628\u06cc \u0628\u0647 \u0631\u0648\u0634 \u067e\u06cc\u0631\u0648\u0633\u06a9\u0627\u0646\u0633 (Pyrosequencing)<\/strong>\/\u067e\u0627\u06cc\u0631\u0648\u0633\u06cc\u06a9\u0648\u0626\u0646\u0633\u06cc\u0646\u06af<\/strong><\/a><\/h4>\n

\"Internet\u00a0\u062a\u06a9\u0646\u06cc\u06a9 \u062a\u0648\u0627\u0644\u06cc \u06cc\u0627\u0628\u06cc \u0645\u0627\u06a9\u0633\u0627\u0645 \u06af\u06cc\u0644\u0628\u0631\u062a \u2013 \u0631\u0648\u0634 \u062a\u062c\u0632\u06cc\u0647 \u0634\u06cc\u0645\u06cc\u0627\u06cc\u06cc \u06cc\u0627 \u0634\u06a9\u0633\u062a \u0632\u0646\u062c\u06cc\u0631<\/a><\/strong><\/h4>\n","protected":false},"excerpt":{"rendered":"

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