{"id":21745,"date":"2020-04-01T12:00:03","date_gmt":"2020-04-01T08:30:03","guid":{"rendered":"http:\/\/darwino.ir\/blogs\/?p=21745"},"modified":"2020-05-21T18:23:10","modified_gmt":"2020-05-21T14:53:10","slug":"illumina-sequencing-technology","status":"publish","type":"post","link":"http:\/\/darwino.ir\/blogs\/illumina-sequencing-technology\/","title":{"rendered":"\u062a\u06a9\u0646\u0648\u0644\u0648\u0698\u06cc\/\u0631\u0648\u0634 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0627\u06cc\u0644\u0648\u0645\u06cc\u0646\u0627 (Illumina)"},"content":{"rendered":"

\u270d <\/strong>\u0641\u0646\u0627\u0648\u0631\u06cc <\/strong>Illumina<\/strong> \u0627\u0632 \u0631\u0648\u0634 \u0647\u0627\u06cc \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0646\u0633\u0644 \u062f\u0648\u0645 <\/strong>\u00a0<\/strong>(NGS)\u00a0<\/strong><\/h2>\n

\u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc DNA \u0627\u0632 \u0645\u0647\u0645 \u062a\u0631\u06cc\u0646 \u062f\u0633\u062a\u0627\u0648\u0631\u062f\u0647\u0627\u06cc \u0628\u0634\u0631 \u0628\u0647 \u062d\u0633\u0627\u0628 \u0645\u06cc \u0622\u06cc\u062f\u060c \u06a9\u0647 \u0627\u0645\u0631\u0648\u0632\u0647 \u0631\u0648\u0634 \u0647\u0627\u06cc \u0645\u062e\u062a\u0644\u0641\u06cc \u0628\u0647 \u0645\u0646\u0638\u0648\u0631 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f\u0647\u0627 \u0628\u0647 \u06a9\u0627\u0631 \u0645\u06cc \u0631\u0648\u062f. \u062a\u0627\u06a9\u0646\u0648\u0646 \u0686\u0646\u062f\u06cc\u0646 \u0646\u0633\u0644 \u0627\u0632 \u0641\u0646\u0627\u0648\u0631\u06cc \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0628\u0647 \u0648\u062c\u0648\u062f \u0622\u0645\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 \u0628\u0631 \u0627\u0633\u0627\u0633 \u0645\u0627\u0647\u06cc\u062a \u0639\u0645\u0644\u06a9\u0631\u062f \u0648 \u0627\u0637\u0644\u0627\u0639\u0627\u062a \u062e\u0631\u0648\u062c\u06cc \u0637\u0628\u0642\u0647 \u0628\u0646\u062f\u06cc \u0645\u06cc \u0634\u0648\u0646\u062f. <\/strong>\u0628\u0639\u062f \u0627\u0632 \u0631\u0648\u0634 \u0633\u0646\u06af\u0631(sanger)\u00a0 \u06a9\u0647 \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u0646\u0633\u0644 \u0627\u0648\u0644 \u0641\u0646\u0627\u0648\u0631\u06cc \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0634\u0646\u0627\u062e\u062a\u0647 \u0645\u06cc \u0634\u0648\u062f\u060c \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0646\u0633\u0644 \u062f\u0648\u0645 \u062a\u062d\u062a \u0639\u0646\u0648\u0627\u0646(NGS) \u0647\u0645 \u0686\u0648\u0646 Pyrosequencing\u060c Ion Torrent \u060cSOLiD\u00a0 \u060cIllumina \u0628\u0627 \u0633\u0647 \u067e\u06cc\u0634\u0631\u0641\u062a \u0639\u0645\u062f\u0647 \u0645\u0637\u0631\u062d \u0634\u062f \u060c\u06a9\u0647 \u0627\u0632 \u067e\u06cc\u0634\u0631\u0641\u062a \u0647\u0627\u06cc \u0622\u0646 \u0645\u06cc \u062a\u0648\u0627\u0646 \u0645\u0648\u0627\u0631\u062f \u0630\u06cc\u0644 \u0631\u0627 \u0628\u06cc\u0627\u0646 \u0646\u0645\u0648\u062f:<\/strong><\/p>\n

\u06f1) \u0628\u0647 \u0645\u0646\u0638\u0648\u0631 \u0622\u0645\u0627\u062f\u0647 \u0633\u0627\u0632\u06cc \u0642\u0637\u0639\u0627\u062a \u062c\u0647\u062a \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0628\u0647 \u062c\u0627\u06cc \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632\u0633\u06cc\u0633\u062a\u0645 \u06a9\u0644\u0648\u0646\u06cc\u0646\u06af \u0628\u0627\u06a9\u062a\u0631\u06cc\u0627\u06cc\u06cc \u0627\u0632 \u0633\u06cc\u0633\u062a\u0645 \u0647\u0627\u06cc \u062e\u0627\u0631\u062c \u0633\u0644\u0648\u0644\u06cc \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f.<\/p>\n

\u06f2) \u0628\u0647 \u062c\u0627\u06cc\u060c \u0635\u062f\u0647\u0627 \u0648\u0627\u06a9\u0646\u0634\u060c \u0647\u0632\u0627\u0631\u0627\u0646 \u062a\u0627 \u0645\u06cc\u0644\u06cc\u0648\u0646 \u0647\u0627 \u0648\u0627\u06a9\u0646\u0634 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0628\u0647 \u0635\u0648\u0631\u062a \u0647\u0645 \u0632\u0645\u0627\u0646 \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u067e\u0630\u06cc\u0631\u062f.<\/p>\n

\u06f3) \u0646\u062a\u0627\u06cc\u062c \u062e\u0631\u0648\u062c\u06cc \u062d\u0627\u0635\u0644 \u0627\u0632 \u0648\u0627\u06a9\u0646\u0634 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0628\u0647 \u0635\u0648\u0631\u062a \u0645\u0633\u062a\u0642\u06cc\u0645 \u0648 \u0628\u062f\u0648\u0646 \u0646\u06cc\u0627\u0632 \u0628\u0647 \u0627\u0644\u06a9\u062a\u0631\u0648\u0641\u0648\u0631\u0632 \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc \u0645\u06cc \u0634\u0648\u062f.<\/p>\n

\u0627\u0644\u0628\u062a\u0647 \u0631\u0648\u0634 \u0647\u0627\u06cc \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0646\u0633\u0644 \u062f\u0648\u0645 (NGS) \u00a0\u0639\u0644\u06cc\u0631\u063a\u0645 \u067e\u06cc\u0634\u0631\u0641\u062a \u0647\u0627\u06cc \u0628\u0633\u06cc\u0627\u0631\u060c \u0645\u062d\u062f\u0648\u062f\u06cc\u062a \u0647\u0627\u06cc \u0642\u0627\u0628\u0644 \u062a\u0648\u062c\u0647\u06cc \u0646\u06cc\u0632 \u062f\u0627\u0631\u0646\u062f\u060c \u06a9\u0647 \u0627\u0632 \u0645\u0647\u0645 \u062a\u0631\u06cc\u0646 \u0622\u0646 \u0647\u0627 \u06a9\u0648\u062a\u0627\u0647 \u0628\u0648\u062f\u0646 \u0637\u0648\u0644 \u062a\u0648\u0627\u0644\u06cc \u062e\u0648\u0627\u0646\u062f\u0647 \u0634\u062f\u0647 \u0648 \u0627\u0646\u062d\u0631\u0627\u0641 \u0646\u0627\u0634\u06cc \u0627\u0632 \u0641\u0631\u0622\u06cc\u0646\u062f \u062a\u06a9\u062b\u06cc\u0631 \u0627\u0633\u062a \u06a9\u0647 \u0645\u0646\u062c\u0631 \u0628\u0647 \u0645\u062d\u062f\u0648\u062f \u0634\u062f\u0646 \u0627\u0645\u06a9\u0627\u0646 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u06a9\u0644 \u0698\u0646\u0648\u0645 \u0645\u06cc \u0634\u0648\u062f. \u06a9\u0647\u00a0 \u0627\u06cc\u0646 \u0645\u062d\u062f\u0648\u062f\u06cc\u062a \u0647\u0627 \u062a\u0648\u0633\u0637 \u0631\u0648\u0634 \u0647\u0627\u06cc \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0646\u0633\u0644 \u0633\u0648\u0645 \u00a0(TGS<\/strong>)\u00a0 \u0628\u0627 \u0646\u06af\u0627\u0647\u06cc \u0645\u062a\u0641\u0627\u0648\u062a \u0645\u0631\u062a\u0641\u0639 \u0645\u06cc \u06af\u0631\u062f\u062f.<\/p>\n

 <\/p>\n

\"\"<\/p>\n

 <\/p>\n

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\u067e\u0633 \u0627\u0632 \u0627\u0648\u0644\u06cc\u0646 \u0631\u0648\u0634 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0646\u0633\u0644 \u062f\u0648\u0645 \u062a\u062d\u062a \u0639\u0646\u0648\u0627\u0646 Pyrosequencing \u06a9\u0647 \u062f\u0631 \u0633\u0627\u0644 \u06f2\u06f0\u06f0\u06f5 \u0645\u0639\u0631\u0641\u06cc \u06af\u0631\u062f\u06cc\u062f\u060c\u0634\u0631\u06a9\u062a Solexa<\/strong> \u062f\u0631 \u0633\u0627\u0644 \u06f2\u06f0\u06f0\u06f6 \u062f\u0633\u062a\u06af\u0627\u0647 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u062e\u0648\u062f \u062a\u062d\u062a \u0639\u0646\u0648\u0627\u0646 Genome Analyzer II<\/strong> \u0631\u0627 \u0645\u0639\u0631\u0641\u06cc \u06a9\u0631\u062f \u06a9\u0647 \u0633\u0627\u0644 \u0628\u0639\u062f \u0627\u0645\u062a\u06cc\u0627\u0632 \u0622\u0646 \u062a\u0648\u0633\u0637 \u0634\u0631\u06a9\u062a Illumina \u062e\u0631\u06cc\u062f\u0627\u0631\u06cc \u0634\u062f. \u062f\u0631 \u0627\u06cc\u0646 \u0631\u0648\u0634 \u06a9\u0647 \u0646\u0648\u0639\u06cc \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0628\u0647 \u0648\u0633\u06cc\u0644\u0647 \u0633\u0646\u062a\u0632 \u0645\u06cc \u0628\u0627\u0634\u062f\u060c \u0627\u0628\u062a\u062f\u0627 \u0622\u063a\u0627\u0632\u06af\u0631\u0647\u0627\u06cc \u0627\u0644\u06cc\u06af\u0648 \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f\u06cc \u0628\u0647 \u0635\u0648\u0631\u062a \u0645\u062a\u0631\u0627\u06a9\u0645 \u0627\u0632 \u0637\u0631\u06cc\u0642 \u067e\u06cc\u0648\u0646\u062f\u0647\u0627\u06cc \u06a9\u0648\u0627\u0644\u0627\u0646 \u0627\u0632 \u0627\u0646\u062a\u0647\u0627\u06cc ‘\u06f5 \u0628\u0647 \u0642\u0637\u0639\u0627\u062a DNA \u0627\u0644\u06af\u0648 \u0645\u062a\u0635\u0644 \u0645\u06cc \u0634\u0648\u0646\u062f.<\/p>\n

\u0645\u0631\u062d\u0644\u0647 \u062a\u06a9\u062b\u06cc\u0631 \u062f\u0631 \u0627\u06cc\u0646 \u0631\u0648\u0634 \u0628\u0647 \u0635\u0648\u0631\u062a bridge_PCR<\/strong> \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u0634\u0648\u062f \u06a9\u0647 \u062f\u0631 \u0627\u06cc\u0646 \u0645\u0631\u062d\u0644\u0647 \u0627\u0632 \u0647\u0631 \u0642\u0637\u0639\u0647 \u062d\u062f\u0648\u062f \u06cc\u06a9 \u0645\u06cc\u0644\u06cc\u0648\u0646 \u0646\u0633\u062e\u0647 \u062f\u0648 \u0631\u0634\u062a\u0647 \u0627\u06cc \u062d\u0627\u0635\u0644 \u0645\u06cc \u0634\u0648\u062f \u06a9\u0647 \u062f\u0631 \u0646\u0648\u0627\u062d\u06cc \u06a9\u0648\u0686\u06a9 \u0628\u0647 \u0635\u0648\u0631\u062a \u062e\u0648\u0634\u0647 \u0647\u0627\u06cc \u0645\u062a\u0645\u0631\u06a9\u0632 \u0642\u0631\u0627\u0631 \u0645\u06cc \u06af\u06cc\u0631\u0646\u062f . \u062a\u06a9\u062b\u06cc\u0631 \u0634\u062f\u062a \u0633\u06cc\u06af\u0646\u0627\u0644 \u0645\u0648\u0631\u062f \u0646\u06cc\u0627\u0632 \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u062f\u0631 \u0645\u0631\u062d\u0644\u0647 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0631\u0627 \u0636\u0645\u0627\u0646\u062a \u0645\u06cc \u06a9\u0646\u062f. \u067e\u0633 \u0627\u0632 \u0627\u06cc\u0646 \u0645\u0631\u062d\u0644\u0647\u060c \u062e\u0648\u0634\u0647 \u0647\u0627\u06cc \u0648\u0627\u0633\u0631\u0634\u062a \u0634\u062f\u0647 \u0648 \u0628\u0647 \u0635\u0648\u0631\u062a \u062a\u06a9 \u0631\u0634\u062a\u0647 \u0627\u06cc \u062f\u0631 \u0645\u06cc \u0622\u06cc\u0646\u062f \u06a9\u0647 \u062f\u0631 \u0645\u0631\u062d\u0644\u0647 \u0628\u0639\u062f \u0628\u0647 \u06a9\u0627\u0631 \u0645\u06cc \u0631\u0648\u0646\u062f. \u0628\u0647 \u0632\u0628\u0627\u0646 \u0633\u0627\u062f\u0647 \u062a\u0631 \u0645\u06cc \u062a\u0648\u0627\u0646 \u06af\u0641\u062a DNA \u062a\u0627\u0632\u0647 \u0633\u0646\u062a\u0632 \u0634\u062f\u0647 \u0628\u0647 \u0645\u0646\u0638\u0648\u0631 \u0627\u062a\u0635\u0627\u0644 \u0628\u0647 \u0622\u063a\u0627\u0632\u06af\u0631 \u0645\u062c\u0627\u0648\u0631 \u062e\u0645 \u0634\u062f\u0647 \u0648 \u0628\u0647 \u0635\u0648\u0631\u062a \u067e\u0644 \u062f\u0631 \u0645\u06cc \u0622\u06cc\u062f \u0648 \u0645\u062d\u0635\u0648\u0644 \u062a\u06a9\u062b\u06cc\u0631 \u0647\u0631 \u0642\u0637\u0639\u0647 \u0628\u0647 \u0634\u06a9\u0644 \u062e\u0648\u0634\u0647 \u0627\u06cc \u0642\u0631\u0627\u0631 \u0645\u06cc \u06af\u06cc\u0631\u062f.<\/p>\n

\u062f\u0631 \u0645\u0631\u062d\u0644\u0647 \u0628\u0639\u062f\u06cc \u06a9\u0647 \u0645\u0631\u062d\u0644\u0647 \u06cc \u0627\u0635\u0644\u06cc \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0627\u0633\u062a \u06f4 \u0646\u0648\u0639 dNTP \u06a9\u0647 \u0647\u0631 \u06cc\u06a9 \u062f\u0627\u0631\u0627\u06cc \u06cc\u06a9 \u0646\u0648\u0639 \u0631\u0646\u06af \u0641\u0644\u0648\u0631\u0633\u0646\u062a \u0645\u06cc \u0628\u0627\u0634\u0646\u062f \u062a\u0648\u0633\u0637 DNA \u067e\u0644\u06cc\u0645\u0631\u0627\u0632 \u0628\u0647 \u0631\u0634\u062a\u0647 \u0627\u06cc \u06a9\u0647 \u0628\u0627 \u06a9\u0645\u06a9 \u0622\u0646\u0632\u06cc\u0645(Linearization enzyme) \u062a\u06a9 \u0631\u0634\u062a\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a \u0627\u0641\u0632\u0648\u062f\u0647 \u0645\u06cc \u0634\u0648\u0646\u062f. \u0647\u0631 \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f \u0646\u0634\u0627\u0646 \u062f\u0627\u0631 \u06a9\u0647 \u0627\u0646\u062a\u0647\u0627\u06cc ‘\u06f3 \u0622\u0646 \u0645\u0633\u062f\u0648\u062f \u0627\u0633\u062a \u0648\u0642\u062a\u06cc \u0648\u0627\u0631\u062f \u0645\u06cc \u0634\u0648\u062f\u060c \u0648\u0627\u06a9\u0646\u0634 \u0628\u0631\u0627\u06cc \u0645\u062f\u062a\u06cc \u0645\u062a\u0648\u0642\u0641 \u0645\u06cc \u0634\u0648\u062f\u060c \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f\u0647\u0627\u06cc \u0627\u0636\u0627\u0641\u06cc \u0627\u0632 \u0645\u062d\u06cc\u0637 \u0634\u06a9\u0633\u062a\u0647 \u0634\u062f\u0647 \u0648 \u0628\u0647 \u062f\u0648 \u0633\u0645\u062a \u0642\u0637\u0639\u0627\u062a\u060c \u0633\u0627\u0632\u06af\u0627\u0631\u062f\u0647\u0646\u062f\u0647 \u0627\u0641\u0632\u0648\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f.<\/p>\n

\u0633\u067e\u0633 \u0627\u06cc\u0646 \u0642\u0637\u0639\u0627\u062a \u0628\u0647 \u0622\u063a\u0627\u0632\u06af\u0631\u0647\u0627\u06cc \u0645\u062a\u0635\u0644 \u0628\u0647 \u0633\u0637\u062d \u0627\u0633\u0644\u0627\u06cc\u062f flow_cell \u0645\u062a\u0635\u0644 \u0645\u06cc \u0634\u0648\u0646\u062f \u0648 \u062a\u06a9\u062b\u06cc\u0631 \u0645\u06cc \u06af\u0631\u062f\u0646\u062f \u0648 \u0633\u067e\u0633 flow_cell \u06a9\u0647 \u0633\u0637\u062d\u06cc \u0645\u0633\u062a\u062d\u06a9\u0645 \u0645\u06cc \u0628\u0627\u0634\u062f \u0634\u0633\u062a\u0634\u0648 \u062f\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f \u0648 \u062f\u0631 \u0627\u06cc\u0646 \u0647\u0646\u06af\u0627\u0645 \u062a\u0635\u0648\u06cc\u0631 \u062a\u0648\u0633\u0637 \u062f\u0648\u0631\u0628\u06cc\u0646 \u062b\u0628\u062a \u0645\u06cc \u06af\u0631\u062f\u062f \u0648 \u062f\u0631 \u0646\u0647\u0627\u06cc\u062a \u0645\u0633\u062f\u0648\u062f\u06cc\u062a \u0627\u0646\u062a\u0647\u0627\u06cc ‘\u06f3 \u0628\u0627 \u062d\u0630\u0641 \u0631\u0646\u06af \u0641\u0644\u0648\u0631\u0633\u0646\u062a \u0628\u0631\u0637\u0631\u0641 \u0645\u06cc \u0634\u0648\u062f . \u062f\u0631 \u0648\u0627\u0642\u0639 \u0645\u06cc \u062a\u0648\u0627\u0646 \u06af\u0641\u062a \u0645\u0631\u062d\u0644\u0647 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0631\u0648\u0646\u062f\u06cc \u0645\u062a\u0648\u0627\u0644\u06cc \u0648 \u062a\u06a9\u0631\u0627\u0631 \u0634\u0648\u0646\u062f\u0647 \u0627\u0632 \u0648\u0631\u0648\u062f \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f \u0646\u0634\u0627\u0646 \u062f\u0627\u0631\u060c \u0634\u0633\u062a\u0634\u0648\u060c \u062b\u0628\u062a \u062a\u0635\u0648\u06cc\u0631\u060c \u0628\u0631\u0634 \u0646\u0634\u0627\u0646\u0647 \u0648 \u0627\u06cc\u062c\u0627\u062f \u06cc\u06a9 ‘\u06f3 \u0622\u0632\u0627\u062f \u0627\u0633\u062a \u0648 \u062f\u0631 \u0646\u0647\u0627\u06cc\u062a \u0628\u0627 \u0622\u0646\u0627\u0644\u06cc\u0632 \u0631\u0646\u06af \u0647\u0627\u06cc \u062b\u0628\u062a \u0634\u062f\u0647 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u0634\u0648\u062f.<\/p>\n

\u0628\u0631 \u062e\u0644\u0627\u0641 \u0631\u0648\u0634 \u0633\u0627\u0646\u06af\u0631 \u060c \u062f\u0631 \u0627\u06cc\u0646 \u0631\u0648\u0634 \u062e\u0627\u062a\u0645\u0647 \u0632\u0646\u062c\u06cc\u0631\u0647 \u062f\u0627\u0626\u0645\u06cc \u0646\u06cc\u0633\u062a \u0648 \u0628\u0627 \u0628\u0631\u0634 \u0631\u0646\u06af \u0641\u0644\u0648\u0631\u0633\u0646\u062a \u0627\u062f\u0627\u0645\u0647 \u0648\u0627\u06a9\u0646\u0634 \u0627\u0632 \u0633\u0631 \u06af\u0631\u0641\u062a\u0647 \u0645\u06cc \u0634\u0648\u062f \u0628\u0647 \u0647\u0645\u06cc\u0646 \u062e\u0627\u0637\u0631 \u0628\u0647 \u0627\u06cc\u0646 \u0631\u0648\u0634 Cyclic reversible termination<\/strong> \u0645\u06cc \u06af\u0648\u06cc\u0646\u062f.<\/p>\n

\u062f\u0631 \u0631\u0648\u0634 Illumina \u0637\u0648\u0644 \u062a\u0648\u0627\u0644\u06cc \u062e\u0648\u0627\u0646\u062f\u0647 \u0634\u062f\u0647 \u0628\u0647 \u06f3\u06f0\u06f0 \u0628\u0627\u0632 \u0631\u0633\u06cc\u062f\u0647 \u0627\u0633\u062a \u0648 \u062f\u0631 \u062e\u0648\u0627\u0646\u0634 \u062a\u0648\u0627\u0644\u06cc \u0646\u0648\u0627\u062d\u06cc \u0647\u0645\u0648 \u067e\u0644\u06cc\u0645\u0631 \u0646\u0633\u0628\u062a \u0628\u0647 \u0631\u0648\u0634 Pyrosequencing \u0639\u0645\u0644\u06a9\u0631\u062f \u0628\u0647\u062a\u0631\u06cc \u062f\u0627\u0631\u062f. \u0648\u0644\u06cc \u0647\u0645 \u0686\u0646\u0627\u0646 \u0645\u06cc \u062a\u0648\u0627\u0646 \u06af\u0641\u062a \u0627\u0635\u0644\u06cc \u062a\u0631\u06cc\u0646 \u0645\u062d\u062f\u0648\u062f\u06cc\u062a \u0627\u06cc\u0646 \u0631\u0648\u0634 \u0637\u0648\u0644 \u062a\u0648\u0627\u0644\u06cc \u062e\u0648\u0627\u0646\u062f\u0647 \u0634\u062f\u0647 \u0645\u06cc \u0628\u0627\u0634\u062f \u06a9\u0647 \u0628\u0647 \u062e\u0627\u0637\u0631 \u0645\u0632\u0627\u062d\u0645\u062a \u0631\u0646\u06af \u0641\u0644\u0648\u0631\u0633\u0646\u062a \u062f\u0631 \u0641\u0639\u0627\u0644\u06cc\u062a \u0622\u0646\u0632\u06cc\u0645 \u067e\u0644\u06cc \u0645\u0631\u0627\u0632 \u0627\u0633\u062a \u06a9\u0647 \u0628\u0627\u0639\u062b \u06a9\u0627\u0647\u0634 \u06a9\u0627\u0631\u0627\u06cc\u06cc \u0627\u06cc\u0646 \u0631\u0648\u0634 \u062f\u0631 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc de novo<\/strong> \u0645\u06cc \u0634\u0648\u062f.<\/p>\n

\u0647\u0645 \u0627\u06a9\u0646\u0648\u0646 Illumina \u062f\u0633\u062a\u06af\u0627\u0647 \u0647\u0627\u06cc\u06cc \u062a\u0648\u0644\u06cc\u062f \u06a9\u0631\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 \u0639\u0644\u0627\u0648\u0647 \u0628\u0631 \u0622\u0646\u0627\u0644\u06cc\u0632 \u062a\u0648\u0627\u0644\u06cc \u062f\u0631 \u0622\u0646\u0627\u0644\u06cc\u0632 \u062a\u0631\u0646\u0633\u06a9\u0631\u06cc\u067e\u062a\u0648\u0645 \u0648 \u0645\u062a\u06cc\u0644\u0627\u0633\u06cc\u0648\u0646 \u062f\u0631 \u0633\u0637\u0648\u062d \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647 \u0647\u0627\u06cc \u0628\u0627\u0644\u06cc\u0646\u06cc \u0648 \u062a\u062d\u0642\u06cc\u0642\u0627\u062a\u06cc \u06a9\u0627\u0631\u0628\u0631\u062f \u062f\u0627\u0631\u0646\u062f. \u0645\u062a\u062f\u0627\u0648\u0644 \u062a\u0631\u06cc\u0646 \u067e\u0644\u062a\u0641\u0631\u0645 \u0647\u0627\u06cc \u0622\u0646 Miseq \u0647\u0627 \u0648 Hiseq \u0647\u0627 \u0647\u0633\u062a\u0646\u062f \u06a9\u0647 Miseq \u0647\u0627 \u062f\u0631 \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647 \u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0637\u0628\u06cc \u06a9\u0627\u0631\u0628\u0631\u062f \u062f\u0627\u0631\u0646\u062f \u0648 \u0628\u0627 \u0633\u0631\u0639\u062a \u0639\u0645\u0644 \u0628\u0627\u0644\u0627 \u0698\u0646\u0648\u0645 \u0647\u0627\u06cc \u06a9\u0648\u0686\u06a9 \u0645\u062b\u0644 \u0628\u0627\u06a9\u062a\u0631\u06cc \u0647\u0627 \u0648 \u0628\u062e\u0634 \u0647\u0627\u06cc \u062e\u0627\u0635 \u0698\u0646\u0648\u0645 \u0631\u0627 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0645\u06cc \u06a9\u0646\u0646\u062f. \u0627\u0645\u0627 Hiseq\u0647\u0627 \u062f\u0631 \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647 \u0647\u0627\u06cc \u062a\u062d\u0642\u06cc\u0642\u0627\u062a\u06cc \u06a9\u0627\u0631\u0628\u0631\u062f \u062f\u0627\u0631\u0646\u062f \u0648 \u0642\u0627\u062f\u0631\u0646\u062f \u0637\u06cc \u0686\u0646\u062f \u0631\u0648\u0632 \u0627\u0637\u0644\u0627\u0639\u0627\u062a\u06cc \u062f\u0631 \u062d\u062f Tb \u0641\u0631\u0627\u0647\u0645 \u06a9\u0646\u0646\u062f. \u0628\u0631\u0627\u06cc \u0645\u062b\u0627\u0644 Hiseq X Ten \u06a9\u0647 \u062f\u0631 \u0633\u0627\u0644 \u06f2\u06f0\u06f1\u06f4\u00a0 \u0645\u0639\u0631\u0641\u06cc \u0634\u062f \u0642\u0627\u062f\u0631 \u0627\u0633\u062a \u06a9\u0644 \u0698\u0646\u0648\u0645 (WGS)<\/strong> \u0631\u0627 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u06a9\u0646\u062f.<\/p>\n

\u0634\u0631\u06a9\u062a Illumina \u0627\u062f\u0639\u0627 \u06a9\u0631\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 \u0628\u0627 \u067e\u06cc\u0634\u0631\u0641\u062a \u0647\u0627\u06cc \u0645\u062a\u0646\u0648\u0639\u06cc \u06a9\u0647 \u062f\u0631 \u0633\u06cc\u0633\u062a\u0645 \u0646\u0648\u0631\u06cc \u0648 flow_cells\u00a0 \u0627\u06cc\u0646 \u0646\u0633\u062e\u0647 \u0627\u06cc\u062c\u0627\u062f \u06a9\u0631\u062f\u0647 \u0627\u0633\u062a \u062a\u0648\u0627\u0646\u0633\u062a\u0647 \u0628\u0647 \u0647\u062f\u0641 \u06af\u0632\u0627\u0631\u06cc NGHRI \u06a9\u0647 \u062f\u0631 \u0648\u0627\u0642\u0639 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0647\u0631 \u0641\u0631\u062f \u0628\u0648\u062f \u062f\u0633\u062a \u067e\u06cc\u062f\u0627 \u06a9\u0646\u062f \u0648 \u0628\u0627 \u0627\u06cc\u0646 \u062f\u0633\u062a\u0627\u0648\u0631\u062f \u062f\u0631 \u0645\u0637\u0627\u0644\u0639\u0647 \u0698\u0646\u062a\u06cc\u06a9 \u062c\u0645\u0639\u06cc\u062a \u0647\u0627 \u062a\u062d\u0648\u0644\u06cc \u0639\u0638\u06cc\u0645 \u0627\u06cc\u062c\u0627\u062f \u06a9\u0646\u062f \u0648 \u062f\u0631 \u0646\u0647\u0627\u06cc\u062a \u0645\u0641\u0647\u0648\u0645 \u067e\u0632\u0634\u06a9\u06cc \u0634\u062e\u0635\u06cc \u0631\u0627 \u0628\u0647 \u0648\u0627\u0642\u0639\u06cc\u062a \u0646\u0632\u062f\u06cc\u06a9 \u06a9\u0631\u062f\u0647 \u0627\u0633\u062a.<\/p>\n

 <\/p>\n

 <\/p>\n

\"Internet<\/strong>\u00a0 \u00a0\u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0633\u0646\u06af\u0631 \u0628\u0647 \u0631\u0648\u0634 \u0627\u062a\u0648\u0645\u0627\u062a<\/a><\/strong><\/h4>\n

\"Internet \u00a0<\/strong>\u0631\u0648\u0634 \u0633\u0646\u06af\u0631 (sanger) \u2013 \u0646\u0633\u0644 \u0627\u0648\u0644 \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc DNA<\/strong><\/a><\/h4>\n

\"Internet<\/strong>\u062a\u0648\u0627\u0644\u06cc \u06cc\u0627\u0628\u06cc \u0628\u0647 \u0631\u0648\u0634 \u067e\u06cc\u0631\u0648\u0633\u06a9\u0627\u0646\u0633 (Pyrosequencing)<\/strong>\/\u067e\u0627\u06cc\u0631\u0648\u0633\u06cc\u06a9\u0648\u0626\u0646\u0633\u06cc\u0646\u06af<\/strong><\/a><\/h4>\n

\"Internet\u00a0\u062a\u06a9\u0646\u06cc\u06a9 \u062a\u0648\u0627\u0644\u06cc \u06cc\u0627\u0628\u06cc \u0645\u0627\u06a9\u0633\u0627\u0645 \u06af\u06cc\u0644\u0628\u0631\u062a \u2013 \u0631\u0648\u0634 \u062a\u062c\u0632\u06cc\u0647 \u0634\u06cc\u0645\u06cc\u0627\u06cc\u06cc \u06cc\u0627 \u0634\u06a9\u0633\u062a \u0632\u0646\u062c\u06cc\u0631<\/a><\/strong><\/h4>\n","protected":false},"excerpt":{"rendered":"

\u270d \u0641\u0646\u0627\u0648\u0631\u06cc Illumina \u0627\u0632 \u0631\u0648\u0634 \u0647\u0627\u06cc \u062a\u0639\u06cc\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0646\u0633\u0644 \u062f\u0648\u0645 […]<\/p>\n","protected":false},"author":6,"featured_media":21756,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[30],"tags":[3499,3497,2870,3498,779,3502,3500,3503,3501],"blog-category":[3088,3070,3089,3071],"darwinoplus-category":[],"class_list":["post-21745","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog","tag-illumina","tag-ion_torrent","tag-pyrosequencing","tag-solid","tag-___","tag-_____illumina","tag-___illumina","tag-_pdf____illumina","tag-______illumina","blog-category-bioinformatics","blog-category-genetic","blog-category-genomics","blog-category-molecular"],"_links":{"self":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/21745","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/comments?post=21745"}],"version-history":[{"count":14,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/21745\/revisions"}],"predecessor-version":[{"id":21984,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/21745\/revisions\/21984"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media\/21756"}],"wp:attachment":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media?parent=21745"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/categories?post=21745"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/tags?post=21745"},{"taxonomy":"blog-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/blog-category?post=21745"},{"taxonomy":"darwinoplus-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/darwinoplus-category?post=21745"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}