{"id":16825,"date":"2019-10-23T05:18:06","date_gmt":"2019-10-23T05:18:06","guid":{"rendered":"http:\/\/darwino.ir\/blogs\/?p=16825"},"modified":"2020-05-19T17:53:59","modified_gmt":"2020-05-19T14:23:59","slug":"real-time-pcr-troubleshooting-part-ii","status":"publish","type":"post","link":"http:\/\/darwino.ir\/blogs\/real-time-pcr-troubleshooting-part-ii\/","title":{"rendered":"\u0631\u0641\u0639 \u0645\u0634\u06a9\u0644\u0627\u062a \u0627\u062d\u062a\u0645\u0627\u0644\u06cc \u062f\u0631 \u0628\u0631\u0631\u0633\u06cc \u0628\u06cc\u0627\u0646 \u0698\u0646 ( Real Time PCR Troubleshooting) \u2013 \u0628\u062e\u0634 \u062f\u0648\u0645"},"content":{"rendered":"<h2><strong>\u270d <\/strong><strong>\u0686\u06af\u0648\u0646\u0647 \u0645\u0634\u06a9\u0644\u0627\u062a \u0622\u0632\u0645\u0627\u06cc\u0634 \u0628\u0631\u0631\u0633\u06cc \u0628\u06cc\u0627\u0646 \u0698\u0646 \u0631\u0627 \u0628\u0631\u0637\u0631\u0641 \u06a9\u0646\u06cc\u0645\u061f<\/strong><\/h2>\n<p>\u062f\u0631 \u0645\u0637\u0644\u0628 \u06af\u0630\u0634\u062a\u0647 \u0628\u0647 \u0627\u06cc\u0646 \u0645\u0648\u0636\u0648\u0639\u00a0 \u067e\u0631\u062f\u0627\u062e\u062a\u06cc\u0645 \u06a9\u0647 \u0627\u06af\u0631 \u0647\u06cc\u0686 \u0645\u062d\u0635\u0648\u0644\u06cc \u062f\u06cc\u062f\u0647 \u0646\u0634\u0648\u062f \u062f\u0644\u0627\u06cc\u0644 \u0627\u062d\u062a\u0645\u0627\u0644\u06cc \u0686\u06cc\u0633\u062a \u0648 \u0628\u0631\u0627\u06cc \u062d\u0644 \u0627\u06cc\u0646 \u0645\u0634\u06a9\u0644\u0627\u062a \u0686\u0647 \u0628\u0627\u06cc\u062f \u06a9\u0631\u062f\u060c \u062f\u0631 \u0627\u06cc\u0646 \u0645\u0637\u0644\u0628 \u0628\u0631\u062e\u06cc \u062f\u06cc\u06af\u0631 \u0627\u0632 \u0645\u0634\u06a9\u0644\u0627\u062a \u0627\u062d\u062a\u0645\u0627\u0644\u06cc \u0631\u0627 \u0628\u0631\u0631\u0633\u06cc \u062e\u0648\u0627\u0647\u06cc\u0645 \u06a9\u0631\u062f .<\/p>\n<h3><strong>*\u062f\u0631 \u0635\u0648\u0631\u062a\u06cc \u06a9\u0647 \u0686\u0646\u062f\u06cc\u0646 \u0645\u062d\u0635\u0648\u0644 <a href=\"http:\/\/darwino.ir\/pcr-technique\/\">PCR<\/a> \u062a\u0648\u0644\u06cc\u062f \u0634\u0648\u062f \u0645\u06cc \u062a\u0648\u0627\u0646\u062f \u0628\u0647 \u062f\u0644\u0627\u06cc\u0644 \u0632\u06cc\u0631 \u0628\u0627\u0634\u062f:<\/strong><\/h3>\n<p>\u06f1) \u067e\u0631\u0627\u06cc\u0645\u0631 \u0628\u0647 \u0637\u0648\u0631 \u0628\u0647\u06cc\u0646\u0647 \u0637\u0631\u0627\u062d\u06cc \u0646\u0634\u062f\u0647 \u06a9\u0647 \u062f\u0631 \u0627\u06cc\u0646 \u0635\u0648\u0631\u062a \u0628\u0627<strong> BLAST<\/strong> \u06a9\u0631\u062f\u0646 \u0635\u062d\u062a \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc \u062a\u0648\u0627\u0644\u06cc \u0645\u0648\u0631\u062f\u0646\u0638\u0631 \u0631\u0627 \u062a\u0623\u06cc\u06cc\u062f \u06a9\u0646\u06cc\u062f. \u0645\u06cc \u062a\u0648\u0627\u0646 \u0628\u0631\u0627\u06cc \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u062a\u0631 \u0634\u062f\u0646 \u0646\u0633\u0628\u062a \u0628\u0647 \u0647\u062f\u0641 \u0637\u0648\u0644 \u067e\u0631\u0627\u06cc\u0645\u0631 \u0631\u0627 \u0627\u0641\u0632\u0627\u06cc\u0634 \u062f\u0627\u062f.<\/p>\n<p>\u06f2) \u067e\u0631\u0627\u06cc\u0645\u0631 \u0647\u0627 \u062a\u062e\u0631\u06cc\u0628 \u0634\u062f\u0647 \u0627\u0646\u062f \u06a9\u0647 \u0645\u06cc \u062a\u0648\u0627\u0646 \u0622\u0646 \u0647\u0627 \u0631\u0627 \u0631\u0648\u06cc \u0698\u0644 \u067e\u0644\u06cc \u0622\u06a9\u0631\u06cc\u0644 \u0622\u0645\u06cc\u062f \u0628\u0631\u0631\u0633\u06cc \u06a9\u0631\u062f.<\/p>\n<p>\u06f3) \u063a\u0644\u0638\u062a \u0645\u0646\u06cc\u0632\u06cc\u0645 \u0628\u0647\u06cc\u0646\u0647 \u0646\u0634\u062f\u0647 \u0627\u0633\u062a\u060c \u0627\u06cc\u0646 \u063a\u0644\u0638\u062a \u0645\u06cc \u062a\u0648\u0627\u0646\u062f \u0631\u0646\u062c\u06cc \u0628\u06cc\u0646 \u06f1\/\u06f5 \u062a\u0627 \u06f5 \u0645\u06cc\u0644\u06cc \u0645\u0648\u0644\u0627\u0631 \u062f\u0627\u0634\u062a\u0647 \u0628\u0627\u0634\u062f \u06a9\u0647 \u0631\u0646\u062c \u0628\u0647\u06cc\u0646\u0647 \u062f\u0631 \u0628\u06cc\u0634\u062a\u0631 \u0645\u0648\u0627\u0631\u062f \u06f2\/\u06f5 \u0645\u06cc \u0628\u0627\u0634\u062f.<\/p>\n<p>\u06f4) \u062f\u0645\u0627\u06cc \u0627\u062a\u0635\u0627\u0644 \u062e\u06cc\u0644\u06cc \u067e\u0627\u06cc\u06cc\u0646 \u0627\u0633\u062a \u06a9\u0647 \u0645\u06cc \u062a\u0648\u0627\u0646 \u06f2 \u062a\u0627 \u06f3 \u062f\u0631\u062c\u0647 \u0622\u0646 \u0631\u0627 \u0627\u0641\u0632\u0627\u06cc\u0634 \u062f\u0627\u062f.<\/p>\n<p>\u06f5) \u0622\u0644\u0648\u062f\u06af\u06cc DNA \u062f\u0627\u0631\u06cc\u0645 \u06a9\u0647 \u0628\u0627\u06cc\u062f \u0647\u0645\u0647 \u0627\u062c\u0632\u0627\u06cc \u0648\u0627\u06a9\u0646\u0634 \u0631\u0627 \u0627\u0632 \u0627\u06cc\u0646 \u0644\u062d\u0627\u0638 \u0686\u06a9 \u06a9\u0631\u062f. \u0628\u0631\u0627\u06cc \u062c\u0644\u0648\u06af\u06cc\u0631\u06cc \u0627\u0632 \u0622\u0644\u0648\u062f\u06af\u06cc\u060c \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634 \u0631\u0627 \u0646\u06cc\u0632 \u0632\u06cc\u0631 \u0647\u0648\u062f \u062f\u0631\u0633\u062a \u06a9\u0646\u06cc\u062f.<\/p>\n<p>\u06f6) \u062f\u0627\u06cc\u0645\u0631 \u067e\u0631\u0627\u06cc\u0645\u0631 \u062a\u0634\u06a9\u06cc\u0644 \u0634\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 \u0645\u06cc \u062a\u0648\u0627\u0646 \u06cc\u06a9 \u0645\u0631\u062d\u0644\u0647 \u0627\u062e\u062a\u06cc\u0627\u0631\u06cc \u062c\u0645\u0639 \u0622\u0648\u0631\u06cc \u0627\u0637\u0644\u0627\u0639\u0627\u062a \u0628\u0639\u062f \u0627\u0632 \u0645\u0631\u062d\u0644\u0647 \u0637\u0648\u06cc\u0644 \u0633\u0627\u0632\u06cc ( \u062f\u0631 <strong>PCR<\/strong> \u0633\u0647 \u067e\u0644\u0647 \u0627\u06cc ) \u0628\u0647 \u0645\u0646\u0638\u0648\u0631 \u0627\u062c\u062a\u0646\u0627\u0628 \u0627\u0632 \u062f\u0627\u06cc\u0645\u0631 \u067e\u0631\u0627\u06cc\u0645\u0631 \u0627\u0636\u0627\u0641\u0647 \u06a9\u0631\u062f.<\/p>\n<p>\u06f7) \u063a\u0644\u0638\u062a \u0627\u0644\u06af\u0648 \u062e\u06cc\u0644\u06cc \u0632\u06cc\u0627\u062f \u0627\u0633\u062a \u06a9\u0647 \u0628\u0627\u06cc\u062f \u0622\u0646 \u0631\u0627 \u062f\u0631 \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634 \u06a9\u0645 \u06a9\u0631\u062f.<\/p>\n<p>\u06f8) \u063a\u0644\u0638\u062a \u067e\u0631\u0627\u06cc\u0645\u0631 \u0632\u06cc\u0627\u062f \u0627\u0633\u062a \u06a9\u0647 \u0645\u06cc \u062a\u0648\u0627\u0646 \u0627\u06cc\u0646 \u063a\u0644\u0638\u062a \u0631\u0627 \u062f\u0631 \u0633\u0631\u06cc\u0627\u0644 \u0647\u0627\u06cc \u06f2 \u0628\u0631\u0627\u0628\u0631 \u0631\u0642\u062a \u06a9\u0627\u0647\u0634 \u062f\u0627\u062f( \u06f0\/\u06f1 \u0645\u06cc\u0644\u06cc \u0645\u0648\u0644\u0627\u0631\u060c \u06f0\/\u06f0\u06f5 \u0645\u06cc\u0644\u06cc \u0645\u0648\u0644\u0627\u0631\u060c \u06f0\/\u06f0\u06f2\u06f5 \u0645\u06cc\u0644\u06cc \u0645\u0648\u0644\u0627\u0631 \u0648 \u06f0\/\u06f0\u06f1\u06f2\u06f5 \u0645\u06cc\u0644\u06cc \u0645\u0648\u0644\u0627\u0631).<\/p>\n<p>\u06f9) \u0628\u0627 DNA \u0698\u0646\u0648\u0645\u06cc \u0622\u0644\u0648\u062f\u0647 \u0634\u062f\u0647 \u06a9\u0647 \u0628\u0631\u0627\u06cc \u0631\u0641\u0639 \u0627\u06cc\u0646 \u0645\u0634\u06a9\u0644 \u0632\u0645\u0627\u0646\u06cc \u06a9\u0647 \u062f\u0631 \u062d\u0627\u0644 \u0633\u0646\u062c\u0634 \u0628\u06cc\u0627\u0646 <strong>RNA\u00a0<\/strong>\u062f\u0631 \u0646\u0645\u0648\u0646\u0647 \u0647\u0633\u062a\u06cc\u062f \u0648 \u0627\u0632 <a href=\"http:\/\/darwino.ir\/rt-pcr-technique-for-synthesizng-dna\/\"><strong>cDNA<\/strong> <\/a>\u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u0627\u0644\u06af\u0648 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc \u06a9\u0646\u06cc\u062f\u060c \u062a\u0627 \u062c\u0627\u06cc\u06cc \u06a9\u0647 \u0645\u0645\u06a9\u0646 \u0627\u0633\u062a \u0637\u0631\u0627\u062d\u06cc \u067e\u0631\u0627\u06cc\u0645\u0631 \u0628\u0631 \u0627\u0633\u0627\u0633 \u0646\u0648\u0627\u062d\u06cc \u0627\u062a\u0635\u0627\u0644\u0627\u062a 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\u0628\u0627\u0634\u062f (\u062a\u06cc\u0648\u0628 \u062e\u0627\u0644\u06cc \u062f\u0631 \u062f\u0633\u062a\u06af\u0627\u0647 \u0646\u06af\u0630\u0627\u0631\u06cc\u062f).<\/p>\n<p>\u06f3) \u062f\u0645\u0627\u06cc \u0648\u0627\u0633\u0631\u0634\u062a\u0647 \u0627\u0648\u0644\u06cc\u0647 \u0637\u0648\u0644\u0627\u0646\u06cc \u0628\u0648\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 \u0645\u062f\u062a \u0632\u0645\u0627\u0646 \u062f\u0646\u0627\u062a\u0648\u0631\u0627\u0633\u06cc\u0648\u0646 \u0627\u0648\u0644\u06cc\u0647 \u0631\u0627 \u0628\u0647 \u06f2 \u062f\u0642\u06cc\u0642\u0647 \u06a9\u0627\u0647\u0634 \u062f\u0647\u06cc\u062f.<\/p>\n<p>&nbsp;<\/p>\n<h3><strong>*\u0686\u0646\u0627\u0646\u0686\u0647 \u062a\u06a9\u0631\u0627\u0631\u0647\u0627\u06cc \u0648\u0627\u06a9\u0646\u0634 \u0628\u0627 \u0647\u0645 \u062f\u0631 \u0645\u06cc\u0632\u0627\u0646 Ct \u06cc\u0627 \u0627\u0637\u0644\u0627\u0639\u0627\u062a \u062f\u0627\u062f\u0647 \u0634\u062f\u0647 \u0627\u062e\u062a\u0644\u0627\u0641 \u062f\u0627\u0634\u062a\u0647 \u0628\u0627\u0634\u0646\u062f \u0628\u0647 \u062f\u0644\u0627\u06cc\u0644 \u0632\u06cc\u0631 \u0627\u0633\u062a:<\/strong><\/h3>\n<p>\u06f1) \u062e\u0637\u0627\u06cc \u0633\u0645\u067e\u0644 \u06a9\u0631\u062f\u0646 \u0628\u0627\u0639\u062b \u0627\u06cc\u062c\u0627\u062f \u0646\u0648\u0633\u0627\u0646 \u0645\u06cc \u0634\u0648\u062f \u06a9\u0647 \u0628\u0631\u0627\u06cc \u0627\u06cc\u0646 \u06a9\u0627\u0631 \u0628\u0627\u06cc\u062f \u06cc\u06a9 \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634 \u06a9\u0644\u06cc \u062f\u0631\u0633\u062a \u06a9\u0631\u062f \u0648 \u0622\u0646 \u0631\u0627 \u062f\u0631 \u0647\u0631 \u062a\u06cc\u0648\u0628 \u0648\u0627\u06a9\u0646\u0634 \u062a\u0642\u0633\u06cc\u0645 \u06a9\u0631\u062f.<\/p>\n<p>\u06f2) \u0648\u0627\u06a9\u0646\u0634 \u062e\u0648\u0628 \u0645\u062e\u0644\u0648\u0637 \u0646\u0634\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 \u0628\u0627\u06cc\u062f \u0628\u0647 \u0622\u0631\u0627\u0645\u06cc \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634 \u0631\u0627 spin \u06cc\u0627 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f.<\/p>\n<p>&nbsp;<\/p>\n<h3><strong>* \u0627\u06af\u0631 \u0641\u0644\u0648\u0631\u0633\u0646\u0633 \u0642\u0627\u0628\u0644 \u062a\u0634\u062e\u06cc\u0635 \u0646\u06cc\u0633\u062a \u06cc\u0627 \u0645\u062a\u063a\u06cc\u0631 \u0627\u0633\u062a \u0645\u06cc \u062a\u0648\u0627\u0646\u062f \u0628\u0647 \u062f\u0644\u0627\u06cc\u0644 \u0632\u06cc\u0631 \u0628\u0627\u0634\u062f:<\/strong><\/h3>\n<p>\u06f1) \u062f\u0627\u06cc\u0645\u0631 \u067e\u0631\u0627\u06cc\u0645\u0631 \u062a\u0634\u06a9\u06cc\u0644 \u0634\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 \u0647\u0645\u0627\u0646 \u06af\u0648\u0646\u0647 \u06a9\u0647 \u0642\u0628\u0644\u0627\u030b \u0647\u0645 \u06af\u0641\u062a\u0647 \u0634\u062f \u06cc\u06a9 \u0645\u0631\u062d\u0644\u0647 \u0627\u062e\u062a\u06cc\u0627\u0631\u06cc \u062c\u0645\u0639 \u0622\u0648\u0631\u06cc \u0627\u0637\u0644\u0627\u0639\u0627\u062a \u0628\u0639\u062f \u0627\u0632 \u0645\u0631\u062d\u0644\u0647 \u0637\u0648\u06cc\u0644 \u0633\u0627\u0632\u06cc ( \u062f\u0631 PCR \u0633\u0647 \u067e\u0644\u0647 \u0627\u06cc ) \u0628\u0647 \u0645\u0646\u0638\u0648\u0631 \u0627\u062c\u062a\u0646\u0627\u0628 \u0627\u0632 \u062f\u0627\u06cc\u0645\u0631 \u067e\u0631\u0627\u06cc\u0645\u0631 \u0627\u0636\u0627\u0641\u0647 \u06a9\u0631\u062f.<\/p>\n<p>\u06f2) <strong>\u0633\u0627\u06cc\u0628\u0631\u06af\u0631\u06cc\u0646<\/strong> \u062e\u0648\u0628 \u0645\u062e\u0644\u0648\u0637 \u0646\u0634\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 \u062f\u0631 \u0627\u06cc\u0646 \u062d\u0627\u0644\u062a \u0627\u06af\u0631 \u0627\u0632 \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634 \u0622\u0645\u0627\u062f\u0647 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0646\u0645\u06cc \u06a9\u0646\u06cc\u062f\u060c \u062f\u0631 \u0632\u0645\u0627\u0646 \u0633\u0627\u062e\u062a \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634\u060c \u0627\u0632 \u0627\u0636\u0627\u0641\u0647 \u0634\u062f\u0646 \u0633\u0627\u06cc\u0628\u0631 \u06af\u0631\u06cc\u0646 \u0645\u0637\u0645\u0626\u0646 \u0634\u0648\u06cc\u062f.<\/p>\n<p>\u06f3) \u062f\u0633\u062a\u06af\u0627\u0647 PCR \u0622\u0644\u0648\u062f\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 \u062f\u0631 \u0627\u06cc\u0646 \u0635\u0648\u0631\u062a \u0637\u0628\u0642 \u067e\u0631\u0648\u062a\u06a9\u0644 \u0633\u0627\u0632\u0646\u062f\u0647 \u0628\u0631\u0627\u06cc \u0622\u0644\u0648\u062f\u06af\u06cc \u0632\u062f\u0627\u06cc\u06cc \u0627\u0642\u062f\u0627\u0645 \u06a9\u0646\u06cc\u062f.<\/p>\n<p>\u06f4)\u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0628\u0647 \u062d\u062f\u0627\u06a9\u062b\u0631 \u0631\u0633\u06cc\u062f\u0647 \u0648 \u0633\u067e\u0633 \u062f\u0631 \u063a\u0644\u0638\u062a \u0647\u0627\u06cc \u0628\u0627\u0644\u0627 \u0627\u0644\u06af\u0648 \u06a9\u0627\u0647\u0634 \u067e\u06cc\u062f\u0627 \u06a9\u0631\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 \u0628\u0631\u0627\u06cc \u0631\u0641\u0639 \u0627\u06cc\u0646 \u0645\u0634\u06a9\u0644 \u0628\u0627\u06cc\u062f \u0634\u0645\u0627\u0631 \u0633\u06cc\u06a9\u0644 \u0647\u0627\u06cc \u0648\u0627\u06a9\u0646\u0634 \u0631\u0627 \u0628\u0631\u0627\u06cc \u062d\u062f \u067e\u0627\u06cc\u0647 \u06a9\u0627\u0647\u0634 \u062f\u0647\u06cc\u062f \u0648 \u062d\u062f \u067e\u0627\u06cc\u0647 \u0631\u0627 \u062a\u0635\u062d\u06cc\u062d \u06a9\u0646\u06cc\u062f.<\/p>\n<p>&nbsp;<\/p>\n<h3>*<strong> \u0627\u06af\u0631 \u06a9\u0646\u062a\u0631\u0644 \u0628\u062f\u0648\u0646 \u0627\u0644\u06af\u0648 \u0645\u062b\u0628\u062a \u0634\u0648\u062f \u0628\u0647 \u0627\u062d\u062a\u0645\u0627\u0644 \u0632\u06cc\u0627\u062f \u0628\u0647 \u062f\u0644\u0627\u06cc\u0644 \u0632\u06cc\u0631 \u0627\u0633\u062a:<\/strong><\/h3>\n<p>\u06f1) \u0627\u062c\u0632\u0627\u06cc \u0648\u0627\u06a9\u0646\u0634 \u0622\u0644\u0648\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 \u062f\u0631 \u0627\u06cc\u0646 \u0635\u0648\u0631\u062a \u0628\u0627\u06cc\u062f \u0627\u062c\u0632\u0627\u06cc \u0648\u0627\u06a9\u0646\u0634 \u0631\u0627 \u062a\u0639\u0648\u06cc\u0636 \u0648 \u0627\u0632 \u0627\u062c\u0632\u0627\u06cc \u062c\u062f\u06cc\u062f \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u06a9\u0646\u06cc\u062f.<\/p>\n<p>\u06f2) \u0622\u0644\u0648\u062f\u06af\u06cc \u062f\u0631 \u0632\u0645\u0627\u0646 \u0633\u0627\u062e\u062a \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634 \u0631\u062e \u062f\u0627\u062f\u0647 \u0627\u0633\u062a \u06a9\u0647 \u062f\u0631 \u0627\u06cc\u0646 \u0635\u0648\u0631\u062a \u0627\u0642\u062f\u0627\u0645\u0627\u062a \u0627\u062d\u062a\u06cc\u0627\u0637\u06cc \u0631\u0627 \u0631\u0639\u0627\u06cc\u062a \u06a9\u0646\u06cc\u062f \u0628\u0631\u0627\u06cc \u0645\u062b\u0627\u0644 \u0645\u06cc \u062a\u0648\u0627\u0646 \u0627\u0632 \u062a\u06cc\u067e \u0647\u0627\u06cc \u0641\u06cc\u0644\u062a\u0631\u062f\u0627\u0631 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u06a9\u0631\u062f \u0648 \u06cc\u0627 \u0628\u0631\u0627\u06cc \u062c\u0644\u0648\u06af\u06cc\u0631\u06cc \u0627\u0632 \u0627\u0646\u062a\u0642\u0627\u0644 \u0645\u0648\u0627\u062f \u0648\u0627\u06a9\u0646\u0634 \u0642\u0628\u0644\u06cc \u0627\u0632 <strong>UNG<\/strong> \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u06a9\u0631\u062f.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/> <\/strong><a href=\"http:\/\/darwino.ir\/real-time-pcr-troubleshooting-part-one\/\"><strong>\u0631\u0641\u0639 \u0645\u0634\u06a9\u0644\u0627\u062a \u0627\u062d\u062a\u0645\u0627\u0644\u06cc \u062f\u0631 \u0628\u0631\u0631\u0633\u06cc \u0628\u06cc\u0627\u0646 \u0698\u0646 ( Real Time PCR Troubleshooting) \u2013 \u0628\u062e\u0634 \u0627\u0648\u0644<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/> <\/strong><strong>\u0631\u0641\u0639 \u0645\u0634\u06a9\u0644\u0627\u062a \u0627\u062d\u062a\u0645\u0627\u0644\u06cc \u062f\u0631 \u0628\u0631\u0631\u0633\u06cc \u0628\u06cc\u0627\u0646 \u0698\u0646 ( Real Time PCR Troubleshooting) \u2013 \u0628\u062e\u0634 \u062f\u0648\u0645\u00a0<\/strong><\/h4>\n","protected":false},"excerpt":{"rendered":"<p>\u270d \u0686\u06af\u0648\u0646\u0647 \u0645\u0634\u06a9\u0644\u0627\u062a \u0622\u0632\u0645\u0627\u06cc\u0634 \u0628\u0631\u0631\u0633\u06cc \u0628\u06cc\u0627\u0646 \u0698\u0646 \u0631\u0627 \u0628\u0631\u0637\u0631\u0641 \u06a9\u0646\u06cc\u0645\u061f [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":16826,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[30],"tags":[2772,1521,2535,2768,2773,2774,2771,2775,532,564,779,2769,3052,2770,531,1490],"blog-category":[3070,3071],"darwinoplus-category":[],"class_list":{"0":"post-16825","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","6":"hentry","7":"category-blog","8":"tag-blast","9":"tag-cdna","10":"tag-pcr","11":"tag-real_time_pcr____","12":"tag-rna","13":"tag-ung","14":"tag-_dna","15":"tag-__real_time_pcr","16":"tag-_real_time_pcr","17":"tag-___","19":"tag-__real_time_pcr____","20":"tag-3052","21":"tag-_pdf_real_time_pcr_____","23":"tag-____real_time_pcr","24":"blog-category-genetic","25":"blog-category-molecular"},"_links":{"self":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/16825","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/comments?post=16825"}],"version-history":[{"count":12,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/16825\/revisions"}],"predecessor-version":[{"id":21491,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/16825\/revisions\/21491"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media\/16826"}],"wp:attachment":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media?parent=16825"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/categories?post=16825"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/tags?post=16825"},{"taxonomy":"blog-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/blog-category?post=16825"},{"taxonomy":"darwinoplus-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/darwinoplus-category?post=16825"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}