{"id":15521,"date":"2019-08-28T03:23:15","date_gmt":"2019-08-28T03:23:15","guid":{"rendered":"http:\/\/darwino.ir\/blogs\/?p=15521"},"modified":"2020-05-12T20:08:00","modified_gmt":"2020-05-12T16:38:00","slug":"evaluation-of-data-obtained-from-high-resolution-melting-curve-hrm","status":"publish","type":"post","link":"http:\/\/darwino.ir\/blogs\/evaluation-of-data-obtained-from-high-resolution-melting-curve-hrm\/","title":{"rendered":"\u0628\u0631\u0631\u0633\u06cc \u062f\u0627\u062f\u0647 \u0647\u0627\u06cc \u0628\u0647 \u062f\u0633\u062a \u0622\u0645\u062f\u0647 \u0627\u0632 \u0645\u0646\u062d\u0646\u06cc \u0630\u0648\u0628 \u0628\u0627 \u0648\u0636\u0648\u062d \u0628\u0627\u0644\u0627 (HRM)"},"content":{"rendered":"<h2><strong>\u270d \u0622\u0646\u0627\u0644\u06cc\u0632 \u062f\u0627\u062f\u0647 \u0647\u0627 \u0648 \u06af\u0631\u0627\u0641 \u0647\u0627\u06cc HRM<\/strong><\/h2>\n<h3><strong>\u06af\u0631\u0627\u0641 \u062a\u06a9\u062b\u06cc\u0631:<\/strong><\/h3>\n<p>\u062f\u0631 \u0622\u0646\u0627\u0644\u06cc\u0632 HRM \u0627\u0647\u0645\u06cc\u062a \u062a\u06a9\u0631\u0627\u0631 \u067e\u0630\u06cc\u0631\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0646\u0633\u0628\u062a \u0628\u0647 \u0628\u0627\u0632\u062f\u0647 \u0648\u0627\u06a9\u0646\u0634 \u0628\u06cc\u0634\u062a\u0631 \u0627\u0633\u062a (\u062f\u0631 \u0645\u0642\u0627\u06cc\u0633\u0647 \u0628\u0627 <a href=\"http:\/\/darwino.ir\/realtime-pcr\/\"><strong>qPCR<\/strong><\/a>). \u0628\u0631\u0627\u06cc \u0645\u062b\u0627\u0644\u060c \u0622\u0646\u0627\u0644\u06cc\u0632 HRM\u060c \u0632\u0645\u0627\u0646\u06cc \u06a9\u0647 \u062a\u06a9\u062b\u06cc\u0631 \u0642\u0637\u0639\u0647 \u06cc \u0645\u0648\u0631\u062f \u0646\u0638\u0631 \u062f\u06cc\u0631\u062a\u0631 \u0627\u0632 \u0627\u0646\u062a\u0638\u0627\u0631 \u0627\u0646\u062c\u0627\u0645 \u0634\u0648\u062f \u0646\u06cc\u0632 \u0642\u0627\u0628\u0644 \u0627\u062c\u0631\u0627 \u0645\u06cc \u0628\u0627\u0634\u062f. \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u0645\u062b\u0627\u0644\u060c \u0632\u0645\u0627\u0646\u06cc \u06a9\u0647 \u0645\u062d\u062f\u0648\u062f\u06cc\u062a \u0637\u0631\u0627\u062d\u06cc \u067e\u0631\u0627\u06cc\u0645\u0631 \u062f\u0627\u0631\u06cc\u0645 \u06cc\u0627 \u0645\u06cc\u0632\u0627\u0646 \u0627\u0644\u06af\u0648 \u067e\u0627\u06cc\u06cc\u0646 \u0627\u0633\u062a\u060c \u0628\u0647 \u0634\u0631\u0637\u06cc \u06a9\u0647 \u0628\u0631\u0627\u06cc \u062a\u0645\u0627\u0645 \u0646\u0645\u0648\u0646\u0647 \u0647\u0627 \u06cc\u06a9\u0633\u0627\u0646 \u0648 \u0633\u0627\u0632\u06af\u0627\u0631 \u0628\u0627\u0634\u062f\u060c \u06a9\u0646\u062a\u0631\u0644 NTC \u0645\u0646\u0641\u06cc \u0628\u0627\u0634\u062f \u0648 \u062a\u06a9\u062b\u06cc\u0631 \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u0627\u0646\u062c\u0627\u0645 \u0634\u062f\u0647 \u0628\u0627\u0634\u062f.<\/p>\n<h3><strong>\u00a0<\/strong><strong>\u062f\u0627\u062f\u0647 \u0647\u0627\u06cc \u062e\u0627\u0645 \u0645\u0646\u062d\u0646\u06cc \u0630\u0648\u0628:<\/strong><\/h3>\n<p>\u062c\u0645\u0639 \u0622\u0648\u0631\u06cc \u062f\u0627\u062f\u0647 \u0647\u0627 \u062f\u0631 \u0637\u0648\u0644 \u0622\u0646\u0627\u0644\u06cc\u0632 HRM \u0634\u0627\u0645\u0644 \u06cc\u06a9 \u0628\u0627\u0632\u0647 \u0632\u0645\u0627\u0646\u06cc \u062e\u0648\u0627\u0646\u0634 \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u0642\u0628\u0644 \u0627\u0632 \u0630\u0648\u0628 \u0627\u0633\u062a. \u0627\u06cc\u0646 \u0648\u0627\u0631\u06cc\u0627\u0646\u0633 \u0628\u0631\u0627\u06cc \u062a\u062c\u0632\u06cc\u0647 \u0648 \u062a\u062d\u0644\u06cc\u0644 \u062f\u0627\u062f\u0647 \u0645\u0634\u06a9\u0644 \u0645\u06cc \u0628\u0627\u0634\u062f\u060c \u062d\u062a\u06cc \u0627\u06af\u0631 \u0698\u0646\u0648\u062a\u06cc\u067e \u0647\u0627\u06cc \u0645\u062e\u062a\u0644\u0641\u06cc \u0628\u0627 \u0627\u062e\u062a\u0644\u0627\u0641 \u0632\u06cc\u0627\u062f \u062f\u0631 \u0646\u0645\u0648\u0646\u0647 \u0648\u062c\u0648\u062f \u062f\u0627\u0634\u062a\u0647 \u0628\u0627\u0634\u062f. \u0628\u0631\u0627\u06cc \u0622\u0646\u0627\u0644\u06cc\u0631 \u0627\u06cc\u0646 \u062f\u0627\u062f\u0647 \u0647\u0627 \u0628\u0627\u06cc\u062f \u0645\u0646\u0627\u0637\u0642 \u0642\u0628\u0644 \u0648 \u0628\u0639\u062f \u0627\u0632 \u0630\u0648\u0628 \u0631\u0627 \u0628\u0631\u0627\u06cc \u0686\u06cc\u0646\u0634 \u0635\u062d\u06cc\u062d \u062f\u0627\u062f\u0647 \u0647\u0627 \u0627\u0646\u062a\u062e\u0627\u0628 \u06a9\u0631\u062f. \u0645\u0634\u062e\u0635 \u06a9\u0631\u062f\u0646 \u062f\u0648 \u0637\u0631\u0641 \u0646\u0627\u062d\u06cc\u0647 \u06cc \u0630\u0648\u0628 \u0628\u0627\u06cc\u062f \u0637\u0648\u0631\u06cc \u0628\u0627\u0634\u062f \u06a9\u0647 \u0646\u0648\u0627\u062d\u06cc \u0642\u0628\u0644 \u0648 \u0628\u0639\u062f \u0627\u0632 \u0630\u0648\u0628 \u0634\u062f\u06af\u06cc \u0628\u0647 \u062f\u0631\u0633\u062a\u06cc \u0627\u0646\u062a\u062e\u0627\u0628 \u0646\u0634\u0648\u062f\u060c \u0645\u0645\u06a9\u0646 \u0627\u0633\u062a \u06a9\u0647 \u0646\u06cc\u0627\u0632 \u0628\u0627\u0634\u062f \u0622\u0646\u0627\u0644\u06cc\u0632 HRM \u0631\u0627 \u062f\u0648\u0628\u0627\u0631\u0647 \u062a\u06a9\u0631\u0627\u0631 \u06a9\u0646\u06cc\u062f.<\/p>\n<h3><strong>\u00a0<\/strong><strong>\u06af\u0631\u0627\u0641 \u0646\u0631\u0645\u0627\u0644\u06cc\u0632\u0647 \u0634\u062f\u0647:<\/strong><\/h3>\n<p>\u062f\u0631 \u0645\u0646\u062d\u0646\u06cc \u062f\u0627\u062f\u0647 \u0647\u0627\u06cc \u062e\u0627\u0645 \u067e\u0631\u0627\u06a9\u0646\u062f\u06af\u06cc \u0641\u0644\u0648\u0631\u0633\u0646\u0633 \u062f\u06cc\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f\u060c \u0627\u0645\u0627 \u062f\u0631 \u06af\u0631\u0627\u0641 \u0646\u0631\u0645\u0627\u0644\u06cc\u0632\u0647 \u0634\u062f\u0647 \u062a\u063a\u06cc\u06cc\u0631\u0627\u062a \u0631\u0646\u062c \u062f\u0645\u0627\u06cc\u06cc \u0646\u0627\u062d\u06cc\u0647 \u0630\u0648\u0628 \u0645\u0634\u0627\u0647\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f\u060c \u06a9\u0647 \u0645\u06cc \u062a\u0648\u0627\u0646 \u0698\u0646\u0648\u062a\u06cc\u067e \u0647\u0627\u06cc \u0645\u062e\u062a\u0644\u0641 \u0631\u0627 \u0645\u0634\u062e\u0635 \u06a9\u0631\u062f\u060c \u0627\u0645\u0627 \u0647\u0646\u0648\u0632 \u0647\u0645\u0648\u0632\u06cc\u06af\u0648\u062a \u0628\u0648\u062f\u0646 \u0646\u0645\u0648\u0646\u0647 \u0647\u0627 \u0628\u0647 \u0633\u062e\u062a\u06cc \u0642\u0627\u0628\u0644 \u062a\u0641\u06a9\u06cc\u06a9 \u0645\u06cc \u0628\u0627\u0634\u062f.<\/p>\n<h3><strong>\u06af\u0631\u0627\u0641 \u062a\u0645\u0627\u06cc\u0632:<\/strong><\/h3>\n<p>\u0627\u06cc\u0646 \u06af\u0631\u0627\u0641 \u0627\u0632 \u0637\u0631\u06cc\u0642 \u062a\u0641\u0631\u06cc\u0642 \u062f\u0627\u062f\u0647 \u0647\u0627\u06cc \u0641\u0644\u0648\u0631\u0633\u0646\u0633 \u0646\u0631\u0645\u0627\u0644 \u0634\u062f\u0647 \u0627\u0632 \u0645\u06cc\u0632\u0627\u0646 \u062d\u0631\u0627\u0631\u062a \u0647\u0631 \u06a9\u062f\u0627\u0645 \u0627\u0632 \u0646\u0645\u0648\u0646\u0647 \u0647\u0627 \u0646\u0633\u0628\u062a \u0628\u0647 \u0646\u0645\u0648\u0646\u0647 \u06cc Baseline ( \u0698\u0646\u0648\u062a\u06cc\u067e \u062a\u0639\u0631\u06cc\u0641 \u0634\u062f\u0647 \u0627\u06cc \u06a9\u0647 \u0627\u0632 \u0642\u0628\u0644 \u0628\u0647 \u062f\u0633\u062a\u06af\u0627\u0647 \u062f\u0627\u062f\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a \u0648 \u0647\u0631 \u0698\u0646\u0648\u062a\u06cc\u067e\u06cc \u0645\u06cc \u062a\u0648\u0627\u0646\u062f \u0628\u0627\u0634\u062f \u0627\u0645\u0627 \u0645\u0639\u0645\u0648\u0644\u0627 \u06cc\u06a9 \u0647\u0645\u0648\u0632\u06cc\u06af\u0648\u062a \u0627\u0646\u062a\u062e\u0627\u0628 \u0645\u06cc \u0634\u0648\u062f ) \u0628\u0647 \u062f\u0633\u062a \u0645\u06cc \u0622\u06cc\u062f. \u0627\u06cc\u0646 \u06af\u0631\u0627\u0641 \u062f\u0631 \u0648\u0627\u0642\u0639 \u062f\u0627\u062f\u0647 \u0647\u0627\u06cc \u0646\u0631\u0645\u0627\u0644 \u0634\u062f\u0647 \u0631\u0627 \u062a\u062c\u0633\u0645 \u06a9\u0631\u062f\u0647 \u0648 \u0646\u0627\u0645 \u06af\u0630\u0627\u0631\u06cc \u0647\u0631 \u06cc\u06a9 \u0627\u0632 \u0698\u0646\u0648\u062a\u06cc\u067e \u0647\u0627 \u0631\u0627 \u0628\u0647 \u0637\u0648\u0631 \u0627\u062a\u0648\u0645\u0627\u062a\u06cc\u06a9 \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u062f\u0647\u062f\u060c \u0627\u06af\u0631 \u0686\u0647 \u0628\u0647\u062a\u0631 \u0627\u0633\u062a \u06a9\u0647 \u062a\u0641\u0627\u0648\u062a \u0647\u0627\u06cc \u0627\u0639\u0644\u0627\u0645 \u0634\u062f\u0647 \u062f\u0631 \u0632\u0645\u0627\u0646 \u0622\u0646\u0627\u0644\u06cc\u0632 \u062f\u0627\u062f\u0647 \u0647\u0627 \u0686\u06a9 \u0634\u0648\u062f.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h4><a href=\"http:\/\/darwino.ir\/high-resolution-melting-analysis-technique\/\"><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><strong>\u062a\u06a9\u0646\u06cc\u06a9 \u0622\u0646\u0627\u0644\u06cc\u0632 \u0645\u0646\u062d\u0646\u06cc \u0630\u0648\u0628 DNA \u0628\u0627 \u0648\u0636\u0648\u062d \u0628\u0627\u0644\u0627 (HRM)<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><strong>\u0628\u0631\u0631\u0633\u06cc \u062f\u0627\u062f\u0647 \u0647\u0627\u06cc \u0628\u0647 \u062f\u0633\u062a \u0622\u0645\u062f\u0647 \u0627\u0632 \u0645\u0646\u062d\u0646\u06cc \u0630\u0648\u0628 \u0628\u0627 \u0648\u0636\u0648\u062d \u0628\u0627\u0644\u0627 (HRM)<\/strong><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><a href=\"http:\/\/darwino.ir\/common-problems-in-high-temperature-melting-curve-hrm-and-method-modification\/\"><strong>\u0645\u0634\u06a9\u0644\u0627\u062a \u0631\u0627\u06cc\u062c \u062f\u0631 \u0645\u0646\u062d\u0646\u06cc \u0630\u0648\u0628 \u0628\u0627 \u062f\u0645\u0627\u06cc \u0628\u0627\u0644\u0627 (HRM) \u00a0\u0648 \u0627\u0635\u0644\u0627\u062d \u0631\u0648\u0634<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><a href=\"http:\/\/darwino.ir\/colors-and-devices-used-in-hrm\/\"><strong>\u0631\u0646\u06af \u0647\u0627 \u0648 \u062f\u0633\u062a\u06af\u0627\u0647 \u0647\u0627\u06cc \u0645\u0648\u0631\u062f \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u062f\u0631 HRM<\/strong><\/a><\/h4>\n","protected":false},"excerpt":{"rendered":"<p>\u270d \u0622\u0646\u0627\u0644\u06cc\u0632 \u062f\u0627\u062f\u0647 \u0647\u0627 \u0648 \u06af\u0631\u0627\u0641 \u0647\u0627\u06cc HRM \u06af\u0631\u0627\u0641 \u062a\u06a9\u062b\u06cc\u0631: [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":15522,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[30],"tags":[458,2219,2214,564,779,2215,2217,2218,808,531,2220,2216],"blog-category":[3070,3071],"darwinoplus-category":[],"class_list":{"0":"post-15521","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","6":"hentry","7":"category-blog","8":"tag-ntc","9":"tag-qpcr","10":"tag-_________","11":"tag-___","13":"tag-_______","14":"tag-________","15":"tag-_pdf_-_______hrm","16":"tag-____pcr","17":"tag-__real_time_pcr","18":"tag-______pcr_","20":"blog-category-genetic","21":"blog-category-molecular"},"_links":{"self":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/15521","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/comments?post=15521"}],"version-history":[{"count":8,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/15521\/revisions"}],"predecessor-version":[{"id":21980,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/15521\/revisions\/21980"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media\/15522"}],"wp:attachment":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media?parent=15521"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/categories?post=15521"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/tags?post=15521"},{"taxonomy":"blog-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/blog-category?post=15521"},{"taxonomy":"darwinoplus-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/darwinoplus-category?post=15521"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}