{"id":12314,"date":"2019-07-17T04:32:08","date_gmt":"2019-07-17T04:32:08","guid":{"rendered":"http:\/\/darwino.ir\/blogs\/?p=12314"},"modified":"2020-05-12T17:58:59","modified_gmt":"2020-05-12T14:28:59","slug":"real-time-pcr-part-iv","status":"publish","type":"post","link":"http:\/\/darwino.ir\/blogs\/real-time-pcr-part-iv\/","title":{"rendered":"\u062a\u06a9\u0646\u06cc\u06a9 Real time PCR &#8211; \u0628\u062e\u0634 \u0686\u0647\u0627\u0631\u0645(\u0686\u0631\u062e\u0647 \u0627\u0646\u062c\u0627\u0645 \u0648\u0627\u06a9\u0646\u0634)"},"content":{"rendered":"<h2><strong>\u270d \u0686\u0631\u062e\u0647 \u0627\u0646\u062c\u0627\u0645 \u0648\u0627\u06a9\u0646\u0634 \u0648 \u0628\u0631\u0646\u0627\u0645\u0647 \u062f\u0645\u0627\u06cc\u06cc<\/strong><\/h2>\n<p><strong>\u00a0\u062f\u0631 \u0632\u0645\u0627\u0646 \u0627\u0646\u062c\u0627\u0645 \u0648\u0627\u06a9\u0646\u0634 \u062f\u0631 \u062f\u0633\u062a\u06af\u0627\u0647 <\/strong>\u00a0<strong>\u0686\u0647 \u0631\u062e \u0645\u06cc \u062f\u0647\u062f!\u061f<\/strong><\/p>\n<p><strong>\u0647\u0645\u0627\u0646\u0646\u062f <a href=\"http:\/\/darwino.ir\/pcr-technique\/\">PCR<\/a> \u06a9\u06cc\u0641\u06cc \u062f\u0631 \u062f\u0633\u062a\u06af\u0627\u0647 RealTime PCR\u060c \u0628\u0647 \u0637\u0648\u0631 \u062a\u0642\u0631\u06cc\u0628\u06cc \u0647\u0645\u0627\u0646 \u0645\u0631\u0627\u062d\u0644 \u0648\u0627\u06a9\u0646\u0634 \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u06af\u06cc\u0631\u062f\u060c \u0628\u0627 \u0627\u06cc\u0646 \u062a\u0641\u0627\u0648\u062a \u06a9\u0647 \u0634\u0645\u0627 \u0641\u0639\u0644 \u0648 \u0627\u0646\u0641\u0639\u0627\u0644\u0627\u062a \u0635\u0648\u0631\u062a \u06af\u0631\u0641\u062a\u0647 \u062f\u0631 \u0645\u0631\u0627\u062d\u0644 \u062f\u0648\u0645 \u0648 \u0633\u0648\u0645 \u0648\u0627\u06a9\u0646\u0634 \u0631\u0627 \u0645\u06cc<\/strong> <strong>\u062a\u0648\u0627\u0646\u06cc\u062f \u0645\u0634\u0627\u0647\u062f\u0647 \u06a9\u0646\u06cc\u062f.<\/strong><\/p>\n<p><strong>\u06f1 &#8211; \u06cc\u06a9 \u0633\u06cc\u06a9\u0644 \u0627\u062e\u062a\u06cc\u0627\u0631\u06cc \u06f2 \u062f\u0642\u06cc\u0642\u0647 \u0627\u06cc \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f5\u06f0 \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u06af\u0631\u0627\u062f<\/strong><\/p>\n<p>\u0627\u06cc\u0646 \u0633\u06cc\u06a9\u0644 \u0632\u0645\u0627\u0646\u06cc \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f \u06a9\u0647 \u0622\u0646\u0632\u06cc\u0645 UNG (Uracil N-glycosylase) \u0628\u0631\u0627\u06cc \u062d\u0630\u0641 \u0622\u0644\u0648\u062f\u06af\u06cc \u0647\u0627\u06cc \u0642\u0628\u0644\u06cc \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0634\u062f\u0647 \u0648 \u0627\u06cc\u0646 \u0632\u0645\u0627\u0646 \u0628\u0627\u0639\u062b \u063a\u06cc\u0631 \u0641\u0639\u0627\u0644 \u06a9\u0631\u062f\u0646 \u0622\u0646\u0632\u06cc\u0645 UNG \u062f\u0631 \u0648\u0627\u06a9\u0646\u0634 \u062c\u062f\u06cc\u062f \u0645\u06cc \u0634\u0648\u062f.<\/p>\n<p><strong>\u06f2- \u06cc\u06a9 \u0633\u06cc\u06a9\u0644 \u06f2 \u062a\u0627 \u06f1\u06f5 \u062f\u0642\u06cc\u0642\u0647 \u0627\u06cc \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f9\u06f0 \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u06af\u0631\u0627\u062f:<\/strong><\/p>\n<p>\u0627\u06cc\u0646 \u0633\u06cc\u06a9\u0644 \u0628\u0631\u0627\u06cc \u0641\u0639\u0627\u0644 \u06a9\u0631\u062f\u0646 \u0622\u0646\u0632\u06cc\u0645 \u067e\u0644\u06cc \u0645\u0631\u0627\u0632 \u0648 \u0648\u0627\u0633\u0631\u0634\u062a\u0647 \u06a9\u0631\u062f\u0646 \u0642\u0637\u0639\u0647 \u0628\u0631 \u0627\u0633\u0627\u0633 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u0646\u0648\u0639 \u0622\u0646\u0632\u06cc\u0645 \u067e\u0644\u06cc \u0645\u0631\u0627\u0632 \u0648 \u0627\u0646\u062f\u0627\u0632\u0647 \u06cc \u0642\u0637\u0639\u0647 \u06cc \u0645\u0648\u0631\u062f \u0646\u0638\u0631 \u0645\u062a\u063a\u06cc\u0631 \u0627\u0633\u062a.<\/p>\n<p><strong>\u06f3 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\u0633\u0627\u0646\u062a\u06cc\u06af\u0631\u0627\u062f \u0645\u0631\u062d\u0644\u0647 \u0627\u062a\u0635\u0627\u0644 \u0648 \u0637\u0648\u06cc\u0644 \u0633\u0627\u0632\u06cc \u0631\u0627 \u062f\u0631 \u06cc\u06a9 \u067e\u0644\u0647 \u0627\u0646\u062c\u0627\u0645 \u062f\u0647\u06cc\u062f. \u062f\u0644\u06cc\u0644 \u0627\u0646\u062c\u0627\u0645 PCR \u0633\u0647 \u067e\u0644\u0647 \u0627\u06cc \u062f\u0631 \u062f\u0645\u0627\u06cc \u0627\u062a\u0635\u0627\u0644 \u0628\u0627\u0644\u0627\u06cc \u06f6\u06f0 \u062f\u0631\u062c\u0647 \u0647\u0645 \u0628\u0647 \u0639\u0644\u062a \u0627\u06cc\u0646 \u06a9\u0647 \u0628\u0627 \u062a\u0648\u062c\u0647 \u0628\u0647 \u0628\u0647\u062a\u0631\u06cc\u0646 \u0639\u0645\u0644\u06a9\u0631\u062f \u0622\u0646\u0632\u06cc\u0645 DNA \u067e\u0644\u06cc \u0645\u0631\u0627\u0632 \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f7\u06f2 \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u06af\u0631\u0627\u062f \u0627\u0633\u062a \u0627\u06af\u0631 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\u0627\u062e\u062a\u06cc\u0627\u0631\u06cc:<\/strong> \u0632\u0645\u0627\u0646\u06cc \u06a9\u0647 \u062a\u06a9\u062b\u06cc\u0631 \u062f\u0627\u06cc\u0645\u0631 \u067e\u0631\u0627\u06cc\u0645\u0631 \u0646\u06cc\u0632 \u062f\u0627\u0631\u06cc\u062f \u0648 \u0645\u06cc \u062e\u0648\u0627\u0647\u06cc\u062f \u062f\u0627\u062f\u0647 \u0647\u0627\u06cc \u0622\u0646 \u0631\u0627 \u062f\u0631 \u0645\u062d\u0627\u0633\u0628\u0647 \u0646\u06cc\u0627\u0648\u0631\u06cc\u062f \u0645\u06cc \u062a\u0648\u0627\u0646\u06cc\u062f \u062f\u0631\u00a0PCR\u00a0\u0633\u0647 \u0645\u0631\u062d\u0644\u0647 \u0627\u06cc \u06cc\u06a9 \u0645\u0631\u062d\u0644\u0647 \u062c\u0645\u0639 \u0622\u0648\u0631\u06cc \u0627\u0637\u0644\u0627\u0639\u0627\u062a \u06f1\u06f5 \u062b\u0627\u0646\u06cc\u0647 \u0627\u06cc \u0628\u0639\u062f\u0627\u0632 \u0645\u0631\u062d\u0644\u0647 \u0637\u0648\u06cc\u0644 \u0633\u0627\u0632\u06cc \u062f\u0631 \u062f\u0645\u0627\u06cc\u06cc \u06a9\u0647 \u0628\u0627\u0644\u0627\u062a\u0631 \u0627\u0632 Tm \u062f\u0627\u06cc\u0645\u0631 \u067e\u0631\u0627\u06cc\u0645\u0631 \u0628\u0627\u0634\u062f \u0648 \u06f3 \u062f\u0631\u062c\u0647 \u067e\u0627\u06cc\u06cc\u0646 \u062a\u0631 \u0627\u0632 Tm \u0645\u062d\u0635\u0648\u0644 \u0627\u0635\u0644\u06cc PCR \u0627\u0633\u062a \u0627\u0636\u0627\u0641\u0647 \u06a9\u0646\u06cc\u062f. \u0627\u06cc\u0646 \u0631\u0648\u0634 \u0628\u0627\u0639\u062b \u0627\u0641\u0632\u0627\u06cc\u0634 \u062f\u0627\u0645\u0646\u0647 \u0648 \u0627\u0637\u0645\u06cc\u0646\u0627\u0646 \u0628\u06cc\u0634\u062a\u0631 \u0628\u0647 \u062f\u0627\u062f\u0647 \u0647\u0627\u06cc\u06cc \u06a9\u0647 \u062d\u0627\u0648\u06cc \u062f\u0627\u06cc\u0645\u0631 \u067e\u0631\u0627\u06cc\u0645\u0631 \u062f\u0631 \u062a\u06a9\u062b\u06cc\u0631 \u0628\u0648\u062f\u0647 \u0627\u0646\u062f \u0645\u06cc \u0634\u0648\u062f. \u062f\u0631 \u0648\u0627\u0642\u0639 \u062f\u0631 \u062f\u0645\u0627\u06cc \u0628\u06cc\u0646 Tm \u062f\u0627\u06cc\u0645\u0631 \u067e\u0631\u0627\u06cc\u0645\u0631 \u0648 Tm \u0645\u062d\u0635\u0631\u0644 \u0627\u0635\u0644\u064a \u0642\u0637\u0639\u0627\u062a \u062f\u0627\u064a\u0645\u0631 \u0628\u0647 \u0635\u0648\u0631\u062a \u062a\u06a9 \u0631\u0634\u062a\u0647 \u0627\u06cc \u062f\u0631 \u0622\u0645\u062f\u0647 \u0641\u0644\u0648\u0631\u0633\u0646\u0633 \u0622\u0632\u0627\u062f \u0646\u0645\u06cc \u0634\u0648\u062f \u0648 \u062f\u06cc\u06af\u0631 \u0645\u06cc\u0632\u0627\u0646 \u0622\u0646 \u0647\u0627 \u062c\u0632\u0648 \u062f\u0627\u062f\u0647 \u0647\u0627\u06cc \u0627\u0635\u0644\u06cc \u0645\u062d\u0627\u0633\u0628\u0647 \u0646\u0645\u06cc \u0634\u0648\u062f .<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u06f3-<\/strong> \u0645\u0631\u062d\u0644\u0647 \u06cc \u0633\u0648\u0645\u060c \u0645\u0646\u062d\u0646\u06cc \u0622\u0646\u0627\u0644\u06cc\u0632 \u0630\u0648\u0628 \u06a9\u0647 \u062f\u0631 \u0645\u0648\u0631\u062f \u0631\u0646\u06af \u0647\u0627\u06cc \u0622\u0632\u0627\u062f \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u06af\u06cc\u0631\u062f \u0648 \u0627\u06cc\u0646 \u0645\u0631\u062d\u0644\u0647 \u062c\u0632\u0648 \u0642\u0627\u0628\u0644\u06cc\u062a \u0647\u0627\u06cc \u062f\u0633\u062a\u06af\u0627\u0647 RealTime PCR \u0646\u0633\u0628\u062a \u0628\u0647 \u062f\u0633\u062a\u06af\u0627\u0647 \u062a\u0631\u0645\u0648\u0633\u0627\u06cc\u06a9\u0644\u0631 PCR \u0645\u0639\u0645\u0648\u0644\u06cc \u0627\u0633\u062a.<br \/>\n\u0627\u06cc\u0646 \u067e\u0631\u0648\u0633\u0647 \u0634\u0627\u0645\u0644 \u06cc\u06a9 \u0645\u0631\u062d\u0644\u0647 \u062f\u0646\u0627\u062a\u0648\u0631\u0627\u0633\u06cc\u0648\u0646 \u06f9\u06f5 \u062f\u0631\u062c\u0647 \u0628\u0647 \u0645\u062f\u062a \u06f1\u06f5 \u062b\u0627\u0646\u06cc\u0647 \u06a9\u0647 \u062f\u0644\u06cc\u0644 \u0627\u0646\u062c\u0627\u0645 \u0627\u06cc\u0646 \u06a9\u0627\u0631 \u0627\u0637\u0645\u06cc\u0646\u0627\u0646 \u0627\u0632 \u0627\u0646\u062c\u0627\u0645 \u067e\u0631\u0648\u0633\u0647 \u0628\u0631 \u0631\u0648\u06cc \u0642\u0637\u0639\u0647 \u0647\u0627\u06cc \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u0645\u0648\u0631\u062f \u0646\u0638\u0631 \u0627\u0633\u062a \u0633\u067e\u0633 \u06af\u0631\u0645\u0627 \u062f\u0647\u06cc \u062f\u0642\u06cc\u0642 \u0628\u0647 \u0642\u0637\u0639\u0647 \u06cc DNA \u062a\u06a9\u062b\u06cc\u0631 \u06cc\u0627\u0641\u062a\u0647 \u0627\u0632 \u062f\u0645\u0627\u06cc \u06f9\u06f5 \u062f\u0631\u062c\u0647 \u0628\u0647 \u06f6\u06f0 \u062f\u0631\u062c\u0647 \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc \u0634\u0648\u062f \u0647\u0645\u0627\u0646 \u0637\u0648\u0631 \u06a9\u0647 \u06af\u0641\u062a\u0647 \u0634\u062f \u0631\u0646\u06af \u0647\u0627\u06cc \u0622\u0632\u0627\u062f \u0645\u062b\u0644 \u0633\u0627\u06cc\u0628\u0631\u06af\u0631\u06cc\u0646 \u06a9\u0647 \u0631\u0646\u06af \u0647\u0627\u06cc \u062f\u0631\u062c \u0634\u0648\u0646\u062f\u0647 (Intercalating ) \u06af\u0641\u062a\u0647 \u0645\u06cc \u0634\u0648\u0646\u062f \u0632\u0645\u0627\u0646\u06cc \u06a9\u0647 \u0628\u06cc\u0646 \u062f\u0648 \u0631\u0634\u062a\u0647 DNA \u0642\u0631\u0627\u0631 \u0645\u06cc \u06af\u06cc\u0631\u0646\u062f \u0648 \u0628\u0631 \u0627\u062b\u0631 \u062f\u0645\u0627 \u062f\u0648 \u0631\u0634\u062a\u0647 \u06cc DNA \u0627\u0632 \u0647\u0645 \u0628\u0627\u0632 \u0645\u06cc \u0634\u0648\u0646\u062f. \u0631\u0646\u06af \u0647\u0627\u06cc \u0628\u06cc\u0646 \u0631\u0634\u062a\u0647 \u0627\u06cc \u0622\u0632\u0627\u062f \u0645\u06cc \u0634\u0648\u062f \u06a9\u0647 \u0647\u0645\u06cc\u0646 \u0628\u0627\u0639\u062b \u06a9\u0627\u0647\u0634 \u0634\u062f\u062a \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u0622\u0646 \u0647\u0627 \u0645\u06cc \u0634\u0648\u062f \u06a9\u0647 \u0628\u0647 \u0635\u0648\u0631\u062a \u067e\u06cc\u06a9 \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u0628\u0631 \u0631\u0648\u06cc \u0646\u0645\u0648\u062f\u0627\u0631 \u0622\u0646\u0627\u0644\u06cc\u0632 \u0630\u0648\u0628 \u0645\u0634\u0627\u0647\u062f\u0647 \u0645\u06cc \u0634\u0648\u0646\u062f .\u062f\u0631 \u0622\u063a\u0627\u0632 \u0622\u0646\u0627\u0644\u06cc\u0632 \u0627\u0641\u0632\u0627\u06cc\u0634 \u0633\u0637\u062d \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u062f\u0631 \u0646\u0645\u0648\u0646\u0647 \u0628\u0647 \u0639\u0644\u062a \u0648\u062c\u0648\u062f \u0645\u06cc\u0644\u06cc\u0648\u0646 \u0647\u0627 \u06a9\u067e\u06cc \u0627\u0632 \u0642\u0637\u0639\u0647 \u0647\u0627\u06cc \u062a\u0648\u0644\u06cc\u062f\u06cc \u0645\u06cc \u0628\u0627\u0634\u062f\u060c \u0627\u0645\u0627 \u0647\u0631 \u0686\u0647 \u0646\u0645\u0648\u0646\u0647 \u06af\u0631\u0645 \u062a\u0631 \u0645\u06cc \u0634\u0648\u062f \u0648 \u062f\u0648 \u0631\u0634\u062a\u0647 \u0627\u0632 \u0647\u0645 \u0628\u0627\u0632 \u0645\u06cc \u0634\u0648\u062f\u060c \u0645\u06cc\u0632\u0627\u0646 \u062f\u0648 \u0631\u0634\u062a\u0647 \u0627\u06cc DNA \u06a9\u0627\u0647\u0634 \u06cc\u0627\u0641\u062a\u0647 \u0648 \u062f\u0631 \u0646\u062a\u06cc\u062c\u0647 \u0645\u06cc\u0632\u0627\u0646 \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u06a9\u0627\u0647\u0634 \u067e\u06cc\u062f\u0627 \u0645\u06cc \u06a9\u0646\u062f. \u062f\u0633\u062a\u06af\u0627\u0647 \u062f\u0627\u0631\u0627\u06cc \u06cc\u06a9 \u062f\u0648\u0631\u0628\u06cc\u0646 \u0627\u0633\u062a \u06a9\u0647 \u0627\u0633\u0646 \u067e\u0631\u0648\u0633\u0647 \u0631\u0627 \u0628\u0647 \u0648\u0633\u06cc\u0644\u0647 \u06cc \u0627\u0646\u062f\u0627\u0632\u0647 \u06af\u06cc\u0631\u06cc \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u062a\u0645\u0627\u0634\u0627 \u0645\u06cc \u06a9\u0646\u062f\u060c \u062f\u0633\u062a\u06af\u0627\u0647 \u0633\u067e\u0633 \u0628\u0647 \u0633\u0627\u062f\u06af\u06cc \u0627\u0637\u0644\u0627\u0639\u0627\u062a \u0631\u062a \u0628\u0647 \u0635\u0648\u0631\u062a \u06af\u0631\u0627\u0641 \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u0645\u0646\u062d\u0646\u06cc \u0630\u0648\u0628 ( Melting curve ) \u0645\u06cc\u0632\u0627\u0646 \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u062f\u0631 \u0645\u0642\u0627\u0628\u0644 \u062f\u0631\u062c\u0647 \u062d\u0631\u0627\u0631\u062a \u0631\u0627 \u0646\u0634\u0627\u0646 \u0645\u06cc \u062f\u0647\u062f. \u062f\u0645\u0627\u06cc \u0630\u0648\u0628 \u062f\u0648 \u0631\u0634\u062a\u0647 DNA \u062f\u0631 \u062d\u0627\u0644 \u0628\u0627\u0632 \u0634\u062f\u0646 \u0627\u0632 \u0647\u0645 \u06a9\u0627\u0645\u0644\u0627 \u0642\u0627\u0628\u0644 \u067e\u06cc\u0634 \u0628\u06cc\u0646\u06cc \u0627\u0633\u062a \u06a9\u0647 \u0628\u0633\u062a\u06af\u06cc \u0628\u0647 \u062a\u0648\u0627\u0644\u06cc \u0628\u0627\u0632\u0647\u0627\u06cc DNA (\u0637\u0648\u0644 \u0648 \u0645\u06cc\u0632\u0627\u0646 GC ) \u062f\u0627\u0631\u062f.<\/p>\n<p>\u0628\u0639\u062f \u0627\u0632 \u0627\u062a\u0645\u0627\u0645 \u0648\u0627\u06a9\u0646\u0634 PCR \u062f\u0633\u062a\u06af\u0627\u0647 RealTime \u0628\u0647 \u0634\u0645\u0627 \u06cc\u06a9 \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0645\u06cc \u062f\u0647\u062f \u06a9\u0647 \u062f\u0631 \u0628\u06cc\u0634\u062a\u0631 \u062f\u0633\u062a\u06af\u0627\u0647 \u0647\u0627 \u0628\u0647 \u0637\u0648\u0631 \u067e\u06cc\u0634 \u0641\u0631\u0636 \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0628\u0647 \u0635\u0648\u0631\u062a \u0645\u062d\u0648\u0631\u0647\u0627\u06cc \u0633\u06cc\u06a9\u0644 \u0628\u0631 \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 Rn \u06cc\u0627 \u0394Rn \u0646\u0645\u0627\u06cc\u0634 \u062f\u0627\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f \u060cRn \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u062e\u0627\u0645 \u0646\u0627\u0634\u06cc \u0627\u0632 \u06af\u0632\u0627\u0631\u0634\u06af\u0631 \u062a\u0642\u0633\u06cc\u0645 \u0628\u0631 \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u0631\u0646\u06af \u0645\u0631\u062c\u0639 \u0645\u06cc \u0628\u0627\u0634\u062f \u06a9\u0647 <strong>\u0633\u06cc\u06af\u0646\u0627\u0644 \u06af\u0632\u0627\u0631\u0634\u06af\u0631 \u0646\u0631\u0645\u0627\u0644 \u0634\u062f\u0647 Rn (Normalized Reporter signal)<\/strong> \u0646\u0627\u0645\u06cc\u062f\u0647 \u0645\u06cc \u0634\u0648\u062f \u0648 \u0627\u06af\u0631 Rn\u00a0 \u0631\u0627 \u0627\u0632 \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u067e\u0633 \u0632\u0645\u06cc\u0646\u0647 \u06a9\u0645 \u06a9\u0646\u06cc\u062f\u060c \u0394Rn \u062d\u0627\u0635\u0644 \u0645\u06cc \u0634\u0648\u062f.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/>\u00a0<a href=\"http:\/\/darwino.ir\/realtime-pcr\/\">\u062a\u06a9\u0646\u06cc\u06a9\/\u0631\u0648\u0634 RealTime PCR \u2013 \u0628\u062e\u0634 \u0627\u0648\u0644 (\u0627\u0646\u0648\u0627\u0639 \u0631\u0648\u0634\u0647\u0627\u06cc \u0622\u0632\u0645\u0627\u06cc\u0634)<\/a><\/strong><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><a href=\"http:\/\/darwino.ir\/real-time-pcr-section-2\/\"><strong>\u062a\u06a9\u0646\u06cc\u06a9 Real-Time PCR \u2013 \u0628\u062e\u0634 \u062f\u0648\u0645 (\u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a)<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><a href=\"http:\/\/darwino.ir\/realtime-pcr-technique-part-3\/\"><strong>\u062a\u06a9\u0646\u06cc\u06a9 RealTime PCR \u2013 \u0628\u062e\u0634 \u0633\u0648\u0645(\u0645\u0631\u0627\u062d\u0644 \u0627\u0646\u062c\u0627\u0645 \u0648\u0627\u06a9\u0646\u0634)<\/strong><\/a><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><\/strong><strong>\u062a\u06a9\u0646\u06cc\u06a9 Real time PCR \u2013 \u0628\u062e\u0634 \u0686\u0647\u0627\u0631\u0645(\u0686\u0631\u062e\u0647 \u0627\u0646\u062c\u0627\u0645 \u0648\u0627\u06a9\u0646\u0634)<\/strong><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><a href=\"http:\/\/darwino.ir\/realtime-pcr-reproduction-curves\/\">\u062a\u06a9\u06cc\u0646\u06a9 RealTime PCR &#8211; \u0628\u062e\u0634 \u067e\u0646\u062c\u0645(\u0627\u0634\u06a9\u0627\u0644\u0627\u062a \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631 -\u06f1)<\/a><\/strong><\/h4>\n<h4><strong><img loading=\"lazy\" decoding=\"async\" class=\"lzy lazyload--done\" title=\"Internet free icon\" src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" srcset=\"https:\/\/image.flaticon.com\/icons\/svg\/2498\/2498193.svg 4x\" alt=\"Internet free icon\" width=\"24\" height=\"26\" data-src=\"https:\/\/image.flaticon.com\/icons\/png\/128\/2498\/2498193.png\" \/><a href=\"http:\/\/darwino.ir\/real-time-pcr-technique-checking-controls-and-sample\/\">\u062a\u06a9\u06cc\u0646\u06a9 RealTime PCR -\u0628\u062e\u0634 \u0634\u0634\u0645(\u0627\u0634\u06a9\u0627\u0644\u0627\u062a \u0645\u0646\u062d\u0646\u06cc \u062a\u06a9\u062b\u06cc\u0631-\u06f2)<\/a><\/strong><\/h4>\n","protected":false},"excerpt":{"rendered":"<p>\u270d \u0686\u0631\u062e\u0647 \u0627\u0646\u062c\u0627\u0645 \u0648\u0627\u06a9\u0646\u0634 \u0648 \u0628\u0631\u0646\u0627\u0645\u0647 \u062f\u0645\u0627\u06cc\u06cc \u00a0\u062f\u0631 \u0632\u0645\u0627\u0646 \u0627\u0646\u062c\u0627\u0645 [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":12315,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[30],"tags":[567,516,568,566,564,882,1443,1444,1445,1279,1442],"blog-category":[3070,3071],"darwinoplus-category":[],"class_list":{"0":"post-12314","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","6":"hentry","7":"category-blog","8":"tag-normalized_reporter_signal","9":"tag-real_time_pcr","10":"tag-_dna___hot_start_holding_stage","11":"tag--real_time_pcr","12":"tag-___","14":"tag-___real_time_pcr","16":"tag-_pdf__real_time_pcr","17":"tag-__real_time_pcr-","18":"tag-__-_real_time_pcr","19":"blog-category-genetic","20":"blog-category-molecular"},"_links":{"self":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/12314","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/comments?post=12314"}],"version-history":[{"count":15,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/12314\/revisions"}],"predecessor-version":[{"id":21483,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/posts\/12314\/revisions\/21483"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media\/12315"}],"wp:attachment":[{"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/media?parent=12314"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/categories?post=12314"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/tags?post=12314"},{"taxonomy":"blog-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/blog-category?post=12314"},{"taxonomy":"darwinoplus-category","embeddable":true,"href":"http:\/\/darwino.ir\/blogs\/wp-json\/wp\/v2\/darwinoplus-category?post=12314"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}